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您的检索式：作者名="Janet Neisewander"
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| 1 | Cocaine-induced Fos expression is detectable in the frontal cortex and striatum of rats under isoflurane but not α-chloralose anesthesia: Implications for FMRI显示文摘 | Peter R. Kufahl Nathan S. Pentkowski Krista Heintzelman Janet L. Neisewander | 2009 | Journal of Neuroscience Methods2009，，2： | 1 |
| 2 | Cocaine potently blocks neuronal α_(3)β_(4) nicotinic acetylcholine receptors in SH-SY5Y cells显示文摘Cocaine is one of the most abused illicit drugs worldwide.It is well known that the dopamine(DA)transporter is its major target;but cocaine also acts on other targets including nicotinic acetylcholine receptors(nAChRs).In this study,we investigated the effects of cocaine on a special subtype of neuronal nAChR,α3β4-nAChR expressed in native SH-SY5Y cells.α3β4-nAChR-mediated currents were recorded using whole-cell recordings.Drugs were applied using a computer-controlled U-tube drug perfusion system.We showed that bath application of nicotine induced inward currents in a concentration-dependent manner with an EC50 value of 20μM.Pre-treatment with cocaine concentration-dependently inhibited nicotine-induced current with an IC50 of 1.5μM.Kinetic analysis showed that cocaine acceleratedα3β4-nAChR desensitization,which caused a reduction of the amplitude of nicotine-induced currents.Co-application of nicotine and cocaine(1.5μM)depressed the maximum response on the nicotine concentration-response curve without changing the EC50 value,suggesting a non-competitive mechanism.The cocaine-induced inhibition of nicotine response exhibited both voltage-and use-dependence,suggesting an open-channel blocking mechanism.Furthermore,intracellular application of GDP-βS(via recording electrode)did not affect cocaine-induced inhibition,suggesting that cocaine did not alter receptor internalization.Moreover,intracellular application of cocaine(30μM)failed to alter the nicotine response.Finally,cocaine(1.5μM)was unable to inhibit the nicotine-induced inward current in heterologous expressedα6/α3β2β3-nAChRs andα4β2-nAChRs expressed in human SH-EP1 cells.Collectively,our results suggest that cocaine is a potent blocker for nativeα3β4-nAChRs expressed in SH-SY5Y cells. | Ze-gang Ma Nan Jiang Yuan-bing Huang Xiao-kuang Ma Jason Brek Eaton Ming Gao Yong-chang Chang Ronald J Lukas Paul Whiteaker Janet Neisewander Jie Wu | 2020 | Acta Pharmacologica Sinica2020，41，2： | 0 |
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