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您的检索式：作者名="Jayasankar Subramanian"
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| 1 | Phospholipase D inhibition by hexanal is associated with calcium signal transduction events in raspberry显示文摘Raspberry(Rubus spp.)is an economically important crop with a restricted growing season and very limited fruit shelf-life due to its extreme tenderness.In order to prolong its shelf life,an aqueous composition containing hexanal as the key active ingredient(HC)was applied as a preharvest spray during fruit development.The effects of HC were assessed using physiological,biochemical and anatomical parameters on the treated fruits and compared with the effects of mock inoculation which lacked hexanal.Sugars and acidity did not show a significant change in response to HC treatment,while the pulling force(the tension required to detach the berry from the receptacle)significantly improved in the HC-treated fruits,compared to control.Scanning electron microscope(SEM)analysis revealed a high correlation between the presence of rigid epidermal hairs and a stronger degree of attachment between berries and their receptacle in the HC treated fruits.Further,electron micrographs also showed abnormal crystalline depositions on the epidermal drupelets of the treated berries.Energy Dispersive X-ray Spectroscopy(EDS)analysis showed those crystals to be largely composed of calcium.HC treatment also resulted in the reduction of transcript level of three phospholipase D genes,as well as altered expression pattern of five members of the annexin gene family,and four calmodulin-binding transcription activators.Quantification of PLD activity showed that hexanal inhibited PLD activity in treated berries.The potential crosstalk between hexanal,phospholipase D activity and calcium and this crosstalk’s role in delaying fruit softening and in prolonging storage life of fruits shelf life is discussed. | Walid El Kayal Gopinadhan Paliyath J Alan Sullivan Jayasankar Subramanian | 2017 | Horticulture Research2017，4，1： | 0 |
| 2 | Engineered Cleistogamy in Camelina sativa for bioconfinement显示文摘Camelina sativa is a self-pollinating and facultative outcrossing oilseed crop.Genetic engineering has been used to improve camelina yield potential for altered fatty acid composition,modified protein profiles,improved seed and oil yield,and enhanced drought resistance.The deployment of transgenic camelina in the field posits high risks related to the introgression of transgenes into nontransgenic camelina and wild relatives.Thus,effective bioconfinement strategies need to be developed to prevent pollen-mediated gene f low(PMGF)from transgenic camelina.In the present study,we overexpressed the cleistogamy(i.e.f loral petal non-openness)-inducing PpJAZ1 gene from peach in transgenic camelina.Transgenic camelina overexpressing PpJAZ1 showed three levels of cleistogamy,affected pollen germination rates after anthesis but not during anthesis,and caused a minor silicle abortion only on the main branches.We also conducted field trials to examine the effects of the overexpressed PpJAZ1 on PMGF in the field,and found that the overexpressed PpJAZ1 dramatically inhibited PMGF from transgenic camelina to non-transgenic camelina under the field conditions.Thus,the engineered cleistogamy using the overexpressed PpJAZ1 is a highly effective bioconfinement strategy to limit PMGF from transgenic camelina,and could be used for bioconfinement in other dicot species. | Debao Huang Liwei Gao Jeremy McAdams Fangzhou Zhao Hongyan Lu Yonghui Wu Jeremy Martin Sherif MSherif Jayasankar Subramanian Hui Duan Wusheng Liu | 2023 | Horticulture Research2023，10，2： | 0 |
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