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| 1 | Different cava reconstruction techniques in liver transplantation:piggyback versus cava resection显示文摘BACKGROUND: Originally, cava reconstruction(CR) in liver transplantation meant complete resection and reinsertion of the donor cava. Alternatively, preservation of the recipients inferior vena cava(IVC) with side-to-side anastomosis(known as 'piggyback') can be performed. Here, partial clamping maintains blood flow of the IVC, which may improve cardiovascular stability, reduce blood loss and stabilize kidney function. The aim of this study was to compare both techniques with particular focus on kidney function.METHODS: A series of 414 patients who had had adult liver transplantations(2006-2009) were included. Among them,176(42.5%) patients had piggyback and 238 had classical CR operation, 112(27.1%) of the patients underwent CR accompanied with veno-venous bypass(CR-B) and 126(30.4%)without a bypass. The choice of either technique was based on the surgeons' individual preference. Kidney function [serum creatinine, calculated glomerular filtration rate(GFR), RIFLE stages] was assessed over 14 days.RESULTS: Lab-MELD scores were significantly higher in CR-B(22.5±11.0) than in CR(17.3±9.0) and piggyback(18.8±10.0)(P=0.008). Unexpectedly, the incidences of arterial stenoses(P=0.045) and biliary leaks(P=0.042) were significantly increased in piggyback. Preoperative serum creatinine levels were the highest in CR-B [1.45±1.17 vs 1.25±0.85(piggyback)and 1.13±0.60 mg/dL(CR); P=0.033]. Although a worsening of postoperative kidney function was observed among all groups,this was most pronounced in CR-B [creatinine day 14: 1.67±1.40 vs 1.35±0.96(piggyback) and 1.45±1.03 mg/dL(CR); P=0.102].Accordingly, the proportion of patients displaying RIFLE stages≥2 was the highest in CR/CR-B(26%/19%) when compared to piggyback(18%).CONCLUSIONS: Piggyback revealed a shorter warm ischemic time, a reduced blood loss, and a decreased risk of acute kidney failure. Thus, piggyback is a useful technique, which should be applied in standard procedures. When piggyback is unfeasible,cava replacement, which displayed a lower incidence of vascular and biliary complications in our study, remains as a safe alternative. | Volker Schmitz Wenzel Schoening Ines Jelkmann Brigitta Globke Andreas Pascher Marcus Bahra Peter Neuhaus Gero Puhl | 2014 | Hepatobiliary & Pancreatic Diseases International2014，13，3： | 5 |
| 2 | 果树病毒RNA的二氧化硅提取法显示文摘针对果树组织含有较高的多酚和多糖物质,研究建立了适合果树病毒日常进行大量RT-PCR检测时,病毒RNA的快速、经济、高效的提取方法。该方法是基于在适宜条件下,二氧化硅对RNA的吸附和洗脱作用,避免了常规方法使用的酚、氯仿等有机溶剂。在2 h内同时可以进行数十个样品的提取,由于提取的是总RNA,1次提取,1次反转录得到的cDNA样品可以用于各种待检病毒的PCR检测。得到的总RNA和cDNA在-20℃条件下可以长期保存备用,大大提高了核酸的提取效率。 | 阮小凤 Jelkmann Wilhelm 杨勇 | 2004 | 果树学报2004，21，4： | 5 |
| 3 | 改良RNA提取法及樱桃PDV和PNRSV的RT-PCR检测(英文)显示文摘介绍了一种从木本植物组织中获得高质量RNA的快速、简单和高效的核酸提取方法。该方法是基于核酸的二氧化硅捕获,避免了使用苯酚、氯仿等有机溶剂。利用该方法从樱桃组织中提取的总RNA用RT-PCR技术检测PDV,PNRSV均获得成功。从感病植株的一年生枝的叶片、韧皮部及芽组织中扩增出了预期的目的片段,即172和449bp,而健康组织中无此扩增带。该法提取的总RNA用于RT-PCR技术检测,其敏感性至少与商业出售的QiagenRNeasy提取试剂盒相当,但简单经济。 | 阮小凤 Jelkmann Wilhelm 马锋旺 | 2004 | 西北农林科技大学学报(自然科学版)2004，32，8： | 4 |
| 4 | Foveavirus, a new plant virus genus显示文摘 | G.P. Martelli W. Jelkmann | 1998 | Archives of Virology1998，，6： | 2 |
| 5 | Sequence analysis of the capsid protein gene of an isolate of Apple stem grooving virus, and its survey in Southern Brazil显示文摘 | NICKEL O FAJARDO T V M JELKMANN W KUHN G B | 2001 | Fitopatologia Brasileira2001，26，3： | 1 |
| 6 | Interleukin - 1β promotes hypoxia - induced apoptosis of glioblastoma ceils by inhibiting hypoxia - inducible factor - 1 mediated adrenomedullin production 显示文摘 | Sun W Depping R Jelkmann W | 2014 | Cell Death Dis2014，5，： | 1 |
| 7 | Normoxic induction of the hypoxia inducible factor 1 alpha by insulin and interleukin-1 beta involves the phosphatedylino-sitol 3-kinase pathway 显示文摘 | Stiehl DP Jelkmann W Wenger RH | 2002 | FEBS Lett2002，512，13： | 1 |
| 8 | Physiology and pharmacology of erythropoietin 显示文摘 | Jelkmann W | 2013 | Transfus Med Hemother2013，40，5： | 1 |
| 9 | Erythropoietin : structure, control of production, and function 显示文摘 | Jelkmann W | 1992 | Physiol Rev1992，72，： | 1 |
| 10 | A new potexvirus associated with strawberry mild yellow edge disease显示文摘 | Jelkmann W Martin RR Lesemann DE | | J Gen Virol0，71，： | 1 |
| 11 | Characterization and detection of several filamentous viruses of cherry:adaptation of an alternative cloning method(DOP-PCR),and modification of an RNA extraction protocol显示文摘 | Rott M E Jelkmann W | 2001 | European Journal of Plant Pathology2001，107，： | 1 |
| 12 | Molecular biology of erythropoietin显示文摘 | Jelkmann W | 2004 | Intern Med2004，43，8： | 1 |
| 13 | Erythropoietin after a century of re- search: younger than ever 显示文摘 | Jelkmann W | 2007 | Eur J Haematol2007，78，3： | 1 |
| 14 | Detection of four apple viruses by multiplex RT-PCR assays with coamplification of plant mRNA as internal control显示文摘 | Menzel W Jelkmann W Maiss E | 2002 | Journal of Virological Methods2002，99，： | 1 |
| 15 | Hydroxylation of hypoxia-inducible transcription factors and chemical compounds targeting the HIF-alpha hydroxylases显示文摘 | Bruegge K Jelkmann W Metzen E | 2007 | Curr Med Chem2007，14，17： | 1 |
| 16 | Erythropoietin in the control of red cell production 显示文摘 | Jelkmann W Metzen E | 1996 | A nat Anz1996，178，： | 1 |
| 17 | Regulation of erythropoietin production 显示文摘 | Jelkmann W | 2011 | J Phys- iol2011，589，6： | 1 |
| 18 | Dexamethasone im-pairs hypoxia-inducible factor-1 function显示文摘 | Wagner AE Stiehl GH Jelkmann W | 2008 | Biochem Biophys Res Commun2008，372，： | 1 |
| 19 | The role of the liver in the production of thrombopoietincompared with erythropoietin显示文摘 | Jelkmann W | 2001 | Eur J Gastroenterol Hepatol2001，13，7： | 1 |
| 20 | The enigma of the metabolic fate of circulating erythropoietin (Epo) in view of the phannacokinetics of the recombinant drugs rhEpo and NESP显示文摘 | Jelkmann W | 2002 | Eur J Haematol2002，69，56： | 1 |
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