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您的检索式：作者名="Jessica Alpers"
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| 1 | Two mitochondrial phosphatases,PP2c63 and Sal2,are required for posttranslational regulation of the TCA cycle in Arabidopsis显示文摘Protein phosphorylation is a well-established post-translational mechanism that regulates protein functions and metabolic pathways.It is known that several plant mitochondrial proteins are phosphorylated in a reversible manner.However,the identities of the protein kinases/phosphatases involved in this mech-anism and their roles in the regulation of the tricarboxylic acid(TCA)cycle remain unclear.In this study,we isolated and characterized plants lacking two mitochondrially targeted phosphatases(Sal2 and PP2c63)along with pyruvate dehydrogenase kinase(PDK),Protein-protein interaction analysis,quantitative phos-phoproteomics,and enzymatic analyses revealed that PDK specifically regulates pyruvate dehydrogenase complex(PDC),while PP2c63 nonspecifically regulates PDC.When recombinant PP2c63 and Sal2 proteins were added to mitochondria isolated from mutant plants,protein-protein interaction and enzymatic analyses showed that PP2c63 directly phosphorylates and modulates the activity of PDC,while Sal2 only indirectly affects TCA cycle enzymes.Characterization of steady-state metabolite levels and fluxes in the mutant lines further revealed that these phosphatases regulate flux through the TCA cycle,and that altered metabolism in the sa/2 pp2c63 double mutant compromises plant growth.These results are discussed in the context of current models of the control of respiration in plants. | Youjun Zhang Jonas Giese Sandra M.Kerbler Beata Siemiatkowska Leonardo Perez de Souza Jessica Alpers David Barbosa Medeiros Dirk K.Hincha Danilo M.Daloso Mark Stitt Iris Finkemeier Alisdair R.Fernie | 2021 | Molecular Plant2021，14，7： | 0 |
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