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| 1 | A new electroplated Ir/Ir(OH)_x pH electrode and its application in the coastal areas of Newport Harbor, California显示文摘Resulting from the rising levels of atmospheric carbon, ocean acidification has become a global problem. It has significant impacts on the development, survival, growth and physiology of marine organisms. Therefore, a highprecision sensor is urgently needed to measure the pH of sea-water. Iridium wire with a diameter of 0.25 mm is used as the substrate, and an Ir/Ir(OH)_x pH electrode is prepared by a one-step electrochemical method in a Li OH solution at the room temperature. A scanning electron microscope(SEM) observation reveals that it is coated with nanoscale particles. In laboratory tests, the electrode exhibits a very promising pH response, with an ideal Nernst slope(56.14–59.52), fast response, good stability and long life-span in tested pH buffer solutions. For a sea trial,four pH electrodes and one Ag/Ag Cl reference electrode are integrated with a self-made chemical sensor, and a profile detection of nearly 70 m is implemented near Newport Harbor, California on August 3, 2015. The results reflect that the pH value measured by the sensor is very close to the data given by Sea-Bird 911 plus CTD, with a difference value ranging from 0.000 075 to 0.064 719. And the sensor shows a better data matching degree in 0–40m water depth. In addition, the high precision and accuracy of the sensor make it possible to use in the ocean observation field. | ZHANG Xiao YE Ying KAN Yating HUANG Yuanfeng JIA Jianjun ZHAO Yue CHEN Chen-Tung Arthur QIN Huawei | 2017 | Acta Oceanologica Sinica2017，36，5： | 2 |
| 2 | ABNORMAL SHOOT 6 interacts with KATANIN 1 and SHADE AVOIDANCE 4 to promote cortical microtubule severing and ordering in Arabidopsis显示文摘Plant interphase cortical microtubules(cMTs)mediate anisotropic cell expansion in response to environmental and developmental cues.In Arabidopsis thaliana,KATANIN 1(KTN1),the p60 catalytic subunit of the conserved MT-severing enzyme katanin,is essential for cMT ordering and anisotropic cell expansion.However,the regulation of KTN1-mediated cMT severing and ordering remains unclear.In this work,we report that the Arabidopsis IQ67 DOMAIN(IQD)family gene ABNORMAL SHOOT 6(ABS6)encodes a MT-associated protein.Overexpression of ABS6 leads to elongated cotyledons,directional pavement cell expansion,and highly ordered transverse cMT arrays.Genetic suppressor analysis revealed that ABS6-mediated cMT ordering is dependent on KTN1 and SHADE AVOIDANCE 4(SAV4).Live imaging of cMT dynamics showed that both ABS6 and SAV4 function as positive regulators of cMT severing.Furthermore,ABS6 directly interacts with KTN1 and SAV4 and promotes their recruitment to the cMTs.Finally,analysis of loss-of-function mutant combinations showed that ABS6,SAV4,and KTN1 work together to ensure the robust ethylene response in the apical hook of dark-grown seedlings.Together,our findings establish ABS6 and SAV4 as positive regulators of cMT severing and ordering,and highlight the role of cMT dynamics in fine-tuning differential growth in plants. | Yuanfeng Li Meng Deng Haofeng Liu Yan Li Yu Chen Min Jia Hui Xue Jingxia Shao Jun Zhao Yafei Qi Lijun An Fei Yu Xiayan Liu | 2021 | Journal of Integrative Plant Biology2021，63，4： | 2 |
| 3 | Au@Ag-labeled SERS lateral flow assay for highly sensitive detection of allergens in milk显示文摘Casein andα-lactalbumin(α-LA)are the main allergens in cow's milk,which can affect the skin,respiratory system,and gastrointestinal tract,even cause anaphylactic shock.Developing a rapid and sensitive detection method of casein andα-LA is still pursued.Herein,a surface-enhanced Raman scattering based lateral flow assay(SERS-LFA)method for rapid and highly sensitive detection of milk allergens in food was established for effectively preventing allergic symptoms.Gold@silver nanoparticles(Au@Ag NPs)were synthesized as SERS active substrate to prepare the antibody-modified SERS Probe and SERS-LFA strips toward casein andα-LA were assembled according to the sandwich mode.The detection results were calculated according to colorimetric and Raman signal.The introduction of SRES signal significantly increased the sensitivity of detection with the limit of detection(LOD)of 0.19 ng/mL and 1.74 pg/mL,and exhibited an excellent linear relationship within the range of 0.55-791.50 ng/mL and 0.1 pg/mL-100.0 ng/mL for casein andα-LA,respectively.Furthermore,SERS-LFA strips was highly specific with the recovery rates for corresponding80.36%-105.12%and 85.73%-118.22%for casein andα-LA,respectively.Therefore,the SERS-LFA could be in great potential to develop a unique allergen detection method. | Jing Li Jia Xu Yi Pan Yongzhi Zhu Yuanfeng Wang Shouhui Chen Xinlin Wei | 2023 | Food Science and Human Wellness2023，12，3： | 1 |
| 4 | Granular support vector machine based on mixed measure显示文摘 | Wang Wenjian Guo Husheng Jia Yuanfeng | 2013 | Neurocomputing2013，101，5： | 1 |
| 5 | Methanotrophy-driven accumulation of organic carbon in four paddy soils of Bangladesh显示文摘Biological methane oxidation is a crucial process in the global carbon cycle that reduces methane emissions from paddy fields and natural wetlands into the atmosphere.However,soil organic carbon accumulation associated with microbial methane oxidation is poorly understood.Therefore,to investigate methane-derived carbon incorporation into soil organic matter,paddy soils originated from different parent materials(Inceptisol,Entisol,and Alfisol) were collected after rice harvesting from four major rice-producing regions in Bangladesh.Following microcosm incubation with 5%(volume/volume)^(13) CH_(4),soil^(13) C-atom abundances significantly increased from background level of 1.08% to 1.88%–2.78%,leading to a net methane-derived accumulation of soil organic carbon ranging from 120 to 307 mg kg^(-1).Approximately 23.6%–60.0% of the methane consumed was converted to soil organic carbon during microbial methane oxidation.The phylogeny of^(13) C-labeled pmoA(enconding the alpha subunit of the particulate methane monooxygenase) and 16 S rRNA genes further revealed that canonical α(type II) and γ(type I) Proteobacteria were active methane oxidizers.Members within the Methylobacter-and Methylosarcina-affiliated type Ia lineages dominated active methane-oxidizing communities that were responsible for the majority of methane-derived carbon accumulation in all three paddy soils,while Methylocystis-affiliated type IIa lineage was the key contributor in one paddy soil of Inceptisol origin.These results suggest that methanotroph-mediated synthesis of biomass plays an important role in soil organic matter accumulation.This study thus supports the concept that methanotrophs not only consume the greenhouse gas methane but also serve as a key biotic factor in maintaining soil fertility. | Nasrin SULTANA Jun ZHAO Yuanfeng CAI G.K.M.Mustafizur RAHMAN Mohammad Saiful ALAM Mohammad FAHEEM Adrian HO Zhongjun JIA | 2022 | Pedosphere2022，32，2： | 1 |
| 6 | Granular support vector machine based on mixed measure 显示文摘 | Wang Wenjian Guo Husheng Jia Yuanfeng | 2013 | Neuro Computing2013，101，5： | 1 |
| 7 | Characteristics of snow cover distribution along railway subgrade and the protective effect of snow fences显示文摘Railways built in cold,snowy,and lightly populated areas are subjected to wind and snow disasters.In this study,we selected a snow hazard prevention and control section of the Altay–Zhundong Railway in Xinjiang Uygur Autonomous Region of China as the research object.We investigated the deposited snowfall variation characteristics on the two sides and in the embankment pavement area of snow fences with different porosities,fence heights,and arrangement distances using single-factor tests and orthogonal tests based on global atmospheric reanalysis climate data,field survey data,and a multi-phase flow analysis model.The results showed significant differences in the characteristics of snow cover distribution and snow cover thickness between the embankment and the cutting in the absence of snow protection measures.The maximum snow cover thickness of the embankment pavement decreased by 12.6%relative to the cutting pavement.The snow cover thickness of the embankment exhibited an increasing trend from windward shoulder to leeward shoulder,whereas the snow cover thickness of the cutting presented a declining trend from windward shoulder to leeward toe.In the collaborative prevention and control of snow fences and embankments,the three factors can be ranked in terms of their sensitivity to deposited snowfall within the influence scope of snow fences as follows:fence height>arrangement distance>porosity.At the same time,fence height yielded a significant relationship for the influence scope of snow fences(P<0.05).The three factors can also be ranked in terms of their sensitivity to deposited snowfall on the pavement as follows:porosity>fence height>arrangement distance.For the embankment protection of the Altay–Zhundong Railway against wind and snow,snow fence with a porosity of 75%,a fence height of 4.8 m,and an arrangement distance from the embankment of 60 m produced the best snow control effect.By revealing the characteristics of snow cover distribution along railway subgrade and the protective effect of snow fences,this study provides valuable references for the engineering applications of railway construction in areas prone to wind and snow disasters. | LEI Jia CHENG Jianjun GAO Li MA Benteng AN Yuanfeng DONG Hongguang | 2023 | Journal of Arid Land2023，15，8： | 0 |
| 8 | Elevated and atmospheric-level methane consumption by soil methanotrophs of three grasslands in China显示文摘Background:Methane(CH4)oxidation driven by soil aerobic methanotrophs demonstrates the capacity of grassland as a CH4 sink.Methods:In this study,we compared the oxidation characteristics of atmospheric-level and elevated concentration(10%)CH4 in a typical grassland(steppe)on the Loess Plateau,an alpine meadow(meadow)on the Qinghai-Tibet Plateau,and an inland arid-area artificial grassland(pasture)in northwest China and investigated the communities of active methanotrophs and their contribution to CH4 oxidation using DNA-based stable-isotope probing and Illumina Miseq sequencing.Results:The results showed that the oxidation of atmospheric CH4 only occurred in steppe and meadow soils where the USCγgroup of methanotrophs was numerically dominant in the methanotroph community.Pasture soils,with their very low relative abundance of USCγ,did not show atmospheric CH4 oxidation.However,a DNA-stable isotope probing experiment with 10%CH4 indicated that conventional CH4 oxidizers(Methylocaldum and Methylocystis)rather than USCγcommunities assimilated significant amounts of 13CH4 for growth.Conclusions:The CH4 oxidation mechanisms in the three experimental grassland soils varied significantly.The USCγgroup may be obligate oligotrophic microorganisms or their growth requires specific unknown conditions. | Yufang Wang Yuanfeng Cai Fujiang Hou Saman Bowatte Zhongjun Jia | 2023 | Grassland Research2023，2，2： | 0 |
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