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| 1 | The apricot(Prunus armeniaca L.)genome elucidates Rosaceae evolution and beta-carotenoid synthesis显示文摘Apricots,scientifically known as Prunus armeniaca L,are drupes that resemble and are closely related to peaches or plums.As one of the top consumed fruits,apricots are widely grown worldwide except in Antarctica.A high-quality reference genome for apricot is still unavailable,which has become a handicap that has dramatically limited the elucidation of the associations of phenotypes with the genetic background,evolutionary diversity,and population diversity in apricot.DNA from P.armeniaca was used to generate a standard,size-selected library with an average DNA fragment size of~20 kb.The library was run on Sequel SMRT Cells,generating a total of 16.54 Gb of PacBio subreads(N50=13.55 kb).The high-quality P.armeniaca reference genome presented here was assembled using long-read single-molecule sequencing at approximately 70×coverage and 171×Illumina reads(40.46 Gb),combined with a genetic map for chromosome scaffolding.The assembled genome size was 221.9 Mb,with a contig NG50 size of 1.02 Mb.Scaffolds covering 92.88%of the assembled genome were anchored on eight chromosomes.Benchmarking Universal Single-Copy Orthologs analysis showed 98.0%complete genes.We predicted 30,436 protein-coding genes,and 38.28%of the genome was predicted to be repetitive.We found 981 contracted gene families,1324 expanded gene families and 2300 apricot-specific genes.The differentially expressed gene(DEG)analysis indicated that a change in the expression of the 9-cis-epoxycarotenoid dioxygenase(NCED)gene but not lycopene beta-cyclase(LcyB)gene results in a lowβ-carotenoid content in the white cultivar“Dabaixing”.This complete and highly contiguous P.armeniaca reference genome will be of help for future studies of resistance to plum pox virus(PPV)and the identification and characterization of important agronomic genes and breeding strategies in apricot. | Fengchao Jiang Junhuan Zhang Sen Wang Li Yang Yingfeng Luo Shenghan Gao Meiling Zhang Shuangyang Wu Songnian Hu Haoyuan Sun Yuzhu Wang | 2019 | Horticulture Research2019，6，1： | 9 |
| 2 | Syncytin-1 modulates placental trophoblast cell proliferation by promoting G1/S transition显示文摘 | Qiang Huang Jinping Li Fengchao Wang Matthew T. Oliver Tracy Tipton Ya Gao Shi-Wen Jiang | 2013 | Cellular Signalling2013，，4： | 1 |
| 3 | Recent development of mi- crowave induced organic reantion 显示文摘 | Wang Jing Jiang Fengchao | 2008 | Chin J Org Chem2008，22，： | 1 |
| 4 | Reduced syncytin-1 expression in choriocarcinoma BeWo cells activates the calpain1–AIF-mediated apoptosis, implication for preeclampsia显示文摘 | Qiang Huang Haibin Chen Fengchao Wang Brian C. Brost Jinping Li Yu Gao Zongfang Li Ya Gao Shi-Wen Jiang | 2014 | Cellular and Molecular Life Sciences2014，，16： | 1 |
| 5 | Investigation of pressure wave behaviors in the rotational speed effects on a pressure-exchange wave rotor显示文摘A wave rotor is suitable for compact and efficient pressure-exchange between gas flows.This work measured the circumferential pressure distribution of the rotor/stator interfaces and utilized a CFD method to simulate the unsteady pressure waves.The experimental and CFD results showed some slopes in the circumferential pressure distributions,and the slopes indicated the traces of specific unsteady pressure waves.Such traces varied regularly if the rotational speed varied within a range from-11%to+11%off the baseline value,but they were seriously disturbed if the rotational speed varied by-45%from the baseline value.It verified that a pressure wave in a wave rotor tended to keep its pressure ratio and propagation velocity unchanged if the rotational speed varied by a small extent,and that the pressure wave could not keep its propagation patterns if the rotational speed varied by a large extent.Because of the pressure wave behaviors,the wave rotor demonstrated specific regulations of the rotational speed effects on its operational states. | Shining CHAN Huoxing LIU Hang SONG Fengchao LI Chongwen JIANG Zhenxun GAO | 2022 | Chinese Journal of Aeronautics2022，35，5： | 0 |
| 6 | Genome transfer for the prevention of female infertility caused by maternal gene mutation显示文摘Poor oocyte quality is associated with early embryo developmental arrest and infertility.Maternal gene plays crucial roles in the regulation of oocyte maturation,and its mutation is a common cause of female infertility.However,how to improve oocyte quality and develop effective therapy for maternal gene mutation remains elusive.Here,we use Zar1 as an example to assess the feasibility of genome transfer to cure maternal gene mutationecaused female infertility.We first discover that cytoplasmic deficiency primarily leads to Zar1-null embryo developmental arrest by disturbing maternal transcript degradation and minor zygotic genome activation(ZGA)during the maternal-zygotic transition.We next perform genome transfer at the oocyte(spindle transfer or polar body transfer)and zygote(early pronuclear transfer or late pronuclear transfer)stages to validate the feasibility of preventing Zar1 mutationecaused infertility.We finally demonstrate that genome transfer either at the oocyte or at the early pronuclear stage can support normal preimplantation embryo development and produce live offspring.Moreover,those pups grow to adulthood and show normal fertility.Therefore,our findings provide an effective basis of therapies for the treatment of female infertility caused by maternal gene mutation. | Dandan Bai Jin Sun Yanping Jia Jiqing Yin Yalin Zhang Yanhe Li Rui Gao Xiling Du Kunming Li Jiaming Lin Zhifen Tu Yu Wang Jiaping Pan Shanshan Liang Yi Guo Jingling Ruan Xiaochen Kou Yanhong Zhao Hong Wang Cizhong Jiang Fengchao Wang Xiaoming Teng Wenqiang Liu Shaorong Gao | 2020 | Journal of Genetics and Genomics2020，47，6： | 0 |
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