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| 1 | Editing of Rice Isoamylase Gene ISA1 Provides Insights into Its Function in Starch Formation显示文摘Isoamylase 1(ISA1) is an isoamylase-type debranching enzyme which plays a predominant role in amylopectin synthesis. In this study, the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated endonuclease 9(CRISPR/Cas9) system was used to edit ISA1 gene in rice via Agrobacterium-mediated transformation. We identified 36 genetic edited lines from 55 T_0 transgenic events, and classified the mutation forms into 7 types. Of those, two homozygous mutants, cr-isa1-1(type 1, with an adenine insertion) and cr-isa1-2(type 3, with a cytosine deletion) were selected for further analysis. Seed sizes of both cr-isa1-1 and cr-isa1-2 were affected, and the two mutants also displayed a shrunken endosperm with significantly lower grain weight. Electron microscopy analysis showed that abnormal starch granules and amyloplasts were found in cr-isa1-1 and cr-isa1-2 endosperm cells. The contents of total starch, amylose and amylopectin in the endosperm of the cr-isa1 mutants were significantly reduced, whereas sugar content and starch gel consistency were observably increased compared to the wild-type. The gelatinization temperature and starch chain length distributions of the cr-isa1 mutants were also altered. Moreover, transcript levels of most starch synthesis-related genes were significantly lower in cr-isa1 mutants. In conclusion, the results indicated that gene edition of ISA1 affected starch synthesis and endosperm development, and brought potential implications for rice quality breeding. | CHAO Shufen CAI Yicong FENG Baobing JIAO Guiai SHENG Zhonghua LUO Ju TANG Shaoqing WANG Jianlong HU Peisong WEI Xiangjin | 2019 | Rice science2019，26，2： | 10 |
| 2 | LIN28 coordinately promotes nucleolar/ribosomal functions and represses the 2C-like transcriptional program in pluripotent stem cells显示文摘LIN28 is an RNA binding protein with important roles in early embryo development,stem cell differentiation/re-programming,tumorigenesis and metabolism.Previous studies have focused mainly on its role in the cytosol where it interacts with Let-7 microRNA precursors or mRNAs,and few have addressed LIN28's role within the nucleus.Here,we show that LIN28 displays dynamic temporal and spatial expression during murine embryo development.Maternal LIN28 expression drops upon exit from the 2-cell stage,and zygotic LIN28 protein is induced at the forming nucleolus during 4-cell to blas-tocyst stage development,to become dominantly expressed in the cytosol after implantation.In cultured pluripotent stem cells(PSCs),loss of LIN28 led to nucleolar stress and activation of a 2-cell/4-cell-like transcriptional program characterized by the expression of endogenous retrovirus genes.Mechanistically,LIN28 binds to small nucleolar RNAs and rRNA to maintain nucleolar integrity,and its loss leads to nucleolar phase separation defects,ribosomal stress and activation of P53 which in turn binds to and activates 2C transcription factor Dux.LIN28 also resides in a complex containing the nucleolar factor Nucleolin(NCL)and the transcrip-tional repressor TRIM28,and LIN28 loss leads to reduced occupancy of the NCL/TRIM28 complex on the Dux and rDNA loci,and thus de-repressed Dux and reduced rRNA expression.Lin28 knockout cells with nucleolar stress are more likely to assume a slowly cycling,translationally inert and anabolically inactive state,which is a part of previously unappreciated 2C-like transcriptional program.These findings elucidate novel roles for nucleolar LIN28 in PSCs,and a new mechanism linking 2C program and nucleolar functions in PSCs and early embryo development. | Zhen Sun Hua Yu Jing Zhao Tianyu Tan Hongru Pan Yuqing Zhu Lang Chen Cheng Zhang Li Zhang Anhua Lei Yuyan Xu Xianju Bi Xin Huang Bo Gao Longfei Wang Cristina Correia Ming Chen Qiming Sun Yu Feng Li Shen Hao Wu Jianlong Wang Xiaohua Shen George Q.Daley Hu Li Jin Zhang | 2022 | Protein & Cell2022，13，7： | 4 |
| 3 | A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2显示文摘The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus,and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed.On the other hand,several concerning SARS-CoV-2 variants,including Omicron,are required to be identified as soon as the samples are identified as‘positive’.Unfortunately,a traditional PCR test does not allow their specific identification.Herein,for the first time,we have developed MOPCS(Methodologies of Photonic CRISPR Sensing),which combines an optical sensing technology-surface plasmon resonance(SPR)with the‘gene scissors’clustered regularly interspaced short palindromic repeat(CRISPR)technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants.MOPCS is a low-cost,CRISPR/Cas12a-systemempowered SPR gene-detecting platform that can analyze viral RNA,without the need for amplification,within 38 min from sample input to results output,and achieve a limit of detection of 15 fM.MOPCS achieves a highly sensitive analysis of SARS-CoV-2,and mutations appear in variants B.1.617.2(Delta),B.1.1.529(Omicron)and BA.1(a subtype of Omicron).This platform was also used to analyze some recently collected patient samples from a local outbreak in China,identified by the Centers for Disease Control and Prevention.This innovative CRISPR-empowered SPR platform will further contribute to the fast,sensitive and accurate detection of target nucleic acid sequences with single-base mutations. | Zhi Chen Jingfeng Li Tianzhong Li Taojian Fan Changle Meng Chaozhou Li Jianlong Kang Luxiao Chai Yabin Hao Yuxuan Tang Omar AAl-Hartomy Swelm Wageh Abdullah GAl-Sehemi Zhiguang Luo Jiangtian Yu Yonghong Shao Defa Li Shuai Feng William J.Liu Yaqing He Xiaopeng Ma Zhongjian Xie Han Zhang | 2022 | National Science Review2022，9，8： | 3 |
| 4 | Numerieal Prediction of Storm Surge in the Qingdao Area Under the Impact of Climate Change显示文摘A typhoon-induced storm surge simulation system was developed for the Qingdao area, including a typhoon diagnostic model for the generation of wind and pressure fields and a 2D Advanced Circulation(ADCIRC) model for simulating the associated storm surge with a 200 m resolution along the Qingdao coastline. The system was validated by an extreme surge event Typhoon Mamie(8509) and the parameters of Typhoon Mamie were used to investigate the sensitivity of typhoon paths to Qingdao storm surges with four selected paths: the paths of Typhoons Mamie(8509), Opal, 3921 and 2413, the selection being made according to their relative position to Qingdao. Experiments based on the Typhoon Mamie(8509) storm surge were also conducted to study the possible influences of future climate changes, including the sea level rise and sea surface temperature(SST) rise, on storm surges along the Qingdao coast. Storm surge conditions under both present day and future(the end of the 21 st century) climate scenarios associated with the four selected paths were simulated. The results show that with the same intensity, when typhoons follow the paths of 3921 and 2413, they would lead to the most serious disasters in different areas of Qingdao. Sea level and SST affect storm surges in different ways: sea level rise affects storm surge mainly through its influence on the tide amplitude, while the increased SST has direct impact on the intensity of the surges. The possible maximum risk of storm surges in 2100 in the Qingdao area caused by typhoons like Mamie(8509) was also estimated in this study. | FENG Jianlong JIANG Wensheng BIAN Changwei | 2014 | Journal of Ocean University of China2014，13，4： | 3 |
| 5 | The potential distribution of adult Antarctic krill in the Amundsen Sea显示文摘Antarctic krill is the key species of ecological system in the Amundsen Sea.At present,the suitable distribution is unobtainable by scientifi c surveys or data from the fi shery.In this paper,the maximum entropy algorithm(Maxent)was used to obtain the potential distribution of adult Antarctic krill in order to provide useful information and reasonable reference for the policy on protecting potential krill habitats around the Amundsen Sea.Occurrence points and 17 environmental variables were used to simulate the distributions.Results show that the high and moderate suitable habitats lie between 65°S and 72°S in the Amundsen Sea.The high suitable habitat accounts for 8.1%of the total area of the Amundsen Sea.The sea ice persistence(ICE),total phytoplankton(PHYC),and the minimum value of dissolved iron(Fe_min)are the three dominant contributors to the model.Results from the response curves show that Antarctic krill preferred habitats with ICE of 0.42-0.93,PHYC of 2.48-2.77 mmol/m^(3) and Fe_min of(7.10×10^(-5))-(9.45×10^(-5))mmol/m 3.Positive trends existed in the PHYC of the high and moderate suitable habitat,and a positive trend existed in the Fe_min of moderate suitable habitat.However,the probability of presence of Antarctic krill will decrease if the increase of the PHYC and Fe_min continues. | Jianlong FENG Lulu LIU Qiulin LIU Liang ZHAO | 2022 | Journal of Oceanology and Limnology2022，40，4： | 1 |
| 6 | Genetic background effects on QTL and QTL × environment interaction for yield and its component traits as revealed by reciprocal introgression lines in rice显示文摘QTLs for quantitative traits are influenced by genetic background(GB) and environment.Identification of QTL with GB independency and environmental stability is prerequisite for effective marker-assisted selection(MAS). In this study, QTLs and QTL × environment interactions affecting grain yield per plant(GY) and its component traits, filled grain number per panicle(FGN), panicle number per plant(PN) and 1000-grain weight(TGW) across six environments were dissected using two sets of reciprocal introgression lines(ILs) derived from the cross Lemont × Teqing and SNP genotypic data. ANOVA indicated that the differences among genotypes and environments within each set of ILs were highly significant for all traits. A total of 72 distinct QTLs for GY and its component traits including 15 for GY, 25 for FGN, 18 for PN, and 29 for TGW were detected over the six environments. Most QTLs(87.4%) showed significant QTL × environment interactions(QEIs) and appeared to be more or less environment-specific. Among 72 QTLs, 15(20.8%) QTLs and 12(16.7%) QEIs were commonly identified in both backgrounds, indicating QTL especially QEI for yield and its component traits had strong GB effects. Four QTL regions affecting GY and its component traits, including S1269707–S4288071, S16661497–S17511092, and S35861863–S36341768 on chromosome 3, and S4134205–S7643153 on chromosome 5, were detected in both backgrounds and coincided with cloned genes for yield-related traits. These regions can be the targeted in rice breeding for high yield potential through MAS. Application of QTL main effects and their environmental interaction effects in MAS was discussed in detail. | Xiaoqian Wang Yunlong Pang Jian Zhang Qiang Zhang Yonghong Tao Bo Feng Tianqing Zheng Jianlong Xu Zhikang Li | 2014 | The Crop Journal2014，2，6： | 1 |
| 7 | The inactivation of bacteriophages MS2 and PhiX174 by nanoscale zero-valent iron:Resistance difference and mechanisms显示文摘Pathogenic enteric viruses pose a significant risk to human health.Nanoscale zero-valent iron(nZVI),a novel material for environmental remediation,has been shown to be a promising tool for disinfection.However,the existing research has typically utilized MS2 or f2 bacteriophages to investigate the antimicrobial properties of nZVI,and the resistance difference between bacteriophages,which is important for the application of disinfection technologies,is not yet understood.Here,MS2 and PhiX174 containing RNA and DNA,respectively,were used as model viruses to investigate the resistances to nZVI.The bacteriophage inactivation mechanisms were also discussed using TEM images,protein,and nucleic acid analysis.The results showed that an initial concentration of 10^(6)PFU/mL of MS2 could be completely inactivated within 240 min by 40 mg/L nZVI at pH 7,whereas the complete inactivation of PhiX174 could not be achieved by extending the reaction time,increasing the nZVI dosage,or changing the dosing means.This indicates that the resistance of phage PhiX174 to nZVI was much stronger than that of MS2.TEM images indicated that the viral particle shape was distorted,and the capsid shell was ruptured by nZVI.The damage to viral surface proteins in both phages was examined by three-dimensional fluorescence spectrum and sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE).However,the nucleic acid analysis demonstrated that the nucleic acid of MS2,but not PhiX174,was destroyed.It indicated that bacteriophage inactivation was mainly attributed to the damage of nucleic acids. | Rong Cheng Yingying Zhang Tao Zhang Feng Hou Xiaoxin Cao Lei Shi Peiwen Jiang Xiang Zheng Jianlong Wang | 2022 | Frontiers of Environmental Science & Engineering2022，16，8： | 0 |
| 8 | Dynamic event-triggered leader-follower consensus of nonlinear multi-agent systems under directed weighted topology显示文摘This paper studies the dynamic event-triggered leader-follower consensus of nonlinear multi-agent systems(MASs)under directed weighted graph containing a directed spanning tree,and also considers the effects of disturbances and leader of non-zero control inputs in the system.Firstly,a novel distributed control protocol is designed for uncertain disturbances and leader of non-zero control inputs in MASs.Secondly,a novel dynamic event-triggered control(DETC)strategy is proposed,which eliminates the need for continuous communication between agents and reduces communication resources between agents.By introducing dynamic thresholds,the complexity of excluding Zeno behavior within the system is reduced.Finally,the effectiveness of the proposed theory is validated through numerical simulation. | Wu Yue Chen Xiangyong Qiu Jianlong Hu Shunwei Zhao Feng | 2023 | The Journal of China Universities of Posts and Telecommunications2023，30，6： | 0 |
| 9 | The Adsorption of GSH in the Liquid Environment显示文摘The phenomena of molecular adsorption in the nanometer are significant to the research of biological and medical materials, bio-medical engineering and other fields. As | NIU Jianlong WU Xue LI Feng ZUO Zhentao The school of physics, Nankai University,Tianjin,300071,P.R.China | 2009 | 生物物理学报2009，25，S1： | 0 |
| 10 | Adaptive Fixed-Time Synchronization of Delayed Memristor-Based Neural Networks with Discontinuous Activations显示文摘Fixed-time synchronization(FTS)of delayed memristor-based neural networks(MNNs)with discontinuous activations is studied in this paper.Both continuous and discontinuous activations are considered forMNNs.And the mixed delays which are closer to reality are taken into the system.Besides,two kinds of control schemes are proposed,including feedback and adaptive control strategies.Based on some lemmas,mathematical inequalities and the designed controllers,a few synchronization criteria are acquired.Moreover,the upper bound of settling time(ST)which is independent of the initial values is given.Finally,the feasibility of our theory is attested by simulation examples. | Tianyuan Jia Xiangyong Chen Xiurong Yao Feng Zhao Jianlong Qiu | 2023 | Computer Modeling in Engineering & Sciences2023，，1： | 0 |
| 11 | 2 - 3 Dynamics Aspect of Subbarrier Fusion Reaction in Light Heavy Ion Systems显示文摘 | Huang Meirong Zhou Feng Wada Roy Liu Xingquan Lin Weiping Zhao Minghui Wang Jiansong Chen Zhiqiang Ma Chunwang Yang Yanyun Wang Qi VaJunbing Han Jianlong Ma Peng Jin Shilun Bai Zhen Hu Qiang Jin Lei Chen Jiangbo Li Yong | 2012 | IMP & HIRFL Annual Report2012，，1： | 0 |
| 12 | Droplet microfluidic chip for precise monitoring of dynamic solution changes显示文摘In this work,an automated microfluidic chip that uses negative pressure to sample and analyze solutions with high temporal resolution was developed.The chip has a T-shaped channel for mixing the sample with a fluorescent indicator,a flow-focusing channel for generating droplets in oil,and a long storage channel for incubating and detecting the droplets.By monitoring the fluorescence intensity of the droplets,the device could detect changes in solution accurately over time.The chip can generate droplets at frequencies of up to 42 Hz with a mixing ratio of 1:1 and a temporal resolution of 3–6 s.It had excellent linearity in detecting fluorescein solution in the concentration range 1–5μM.This droplet microfluidic chip provides several advantages over traditional methods,including high temporal resolution,stable droplet generation,and faster flow rates.This approach could be applied to monitoring calcium ions with a dynamic range from 102 to 107 nM and a detection limit of 10 nM. | Cong Ma Zehang Gao Jianlong Zhao Shilun Feng | 2023 | Nanotechnology and Precision Engineering2023，6，3： | 0 |
| 13 | Estimates of Reynolds Stress and TKE Production in the Seasonally Stratified East China Sea显示文摘During the two cruises in March and July of 2011,the tidal cycling of turbulent properties and the T/S profiles at the same location in seasonally stratified East China Sea (ECS) were measured synchronously by a bottom-mounted fast sampling ADCP (acoustic Doppler current profiler) and a RBR CTD (RBR-620) profiler.While focusing on the tide-induced and stratification’s impact on mixing,the Reynolds stress and the turbulent kinetic energy (TKE) production rate were calculated using the‘variance method’.In spring,the features of mixing mainly induced by tides were clear when the water column was well-mixed.Velocity shear and turbulent parameters intensified towards the seabed due to the bottom friction.The components of the velocity shear and the Reynolds stress displayed a dominant semi-diurnal variation related to velocity changes caused by the flood and ebb of M2tide.Stratification occurred in summer,and the water column showed a strongly stratified pycnocline with a characteristic squared buoyancy frequency of N2~ (1 6) ×10 3s 2.The components of the velocity shear and the Reynolds stress penetrated upwards very fast from the bottom boundary layer to the whole water column in spring,while in summer they only penetrated to the bottom of the pycnocline with a relatively slow propagation speed.In summer,the TKE production within the pycnocline was comparable with and sometimes larger than that in the well-mixed bottom layer under the pycnocline.Considering the associated high velocity shear,it is speculated that the mixing in the pycnocline is a result of the local velocity shear. | YANG Wei ZHAO Liang XU Peng FENG Jianlong WANG Tao QUAN Qi JIANG Wensheng | 2013 | Journal of Ocean University of China2013，12，4： | 0 |
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