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您的检索式：作者名="Jieling Liang"
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| 1 | A new membrane re-anchored protein originating from GPC3 against hepatomacells HepG2显示文摘 | Dongye Yang Junwen Yang Fanggen Lu Caihong Li Jingbo Yang Jieling Liang | 2011 | Molecular Medicine Reports2011，，6： | 1 |
| 2 | Rapid and sensitive detection of cucumber mosaic virus by reverse transcription loop-mediated isothermal amplification显示文摘Cucumber mosaic virus(CMV),as a member of Cucumovirus genus,is one of the most common lily viruses and has been reported in many countries [1,2].The CMV-infected plants may show developmental abnormalities of leaves,flowers,and bulbs [1].The symptoms will be extremely severe in plants infected by lily symptomless virus(LSV)[1].So far,CMV has posed a threat to cultivars of lily production.Consequently,it is important to develop an efficient approach to achieve rapid and sensitive detection of CMV.Many visual and serological methods have been reported to detect CMV,such as double antibody sandwich enzyme-linked immunosorbent assay [1],immunosorbent electron microscopy [1],enzyme-linked immunosorbent assay(ELISA)and reverse transcriptase-polymerase chain reaction(RT-PCR)[2].However,these methods are time-consuming and require skilled operator and specialized equipment.Loop-mediated isothermal amplification(LAMP)method provides a possible solution for these problems [3].LAMP was developed by Notomi et al.[4] with high specificity and sensitivity because of Bst DNA polymerase.LAMP method not only is suitable for field and large-scale detection of animal virus with more cost-effective but also could be applied to detect foodborne bacterial pathogens and other viruses with high performances [5].For a naked-eye inspection,SYBR Green I was added to the LAMP reaction products to visualize the results.In this study,we developed a rapid and sensitive LAMP method to detect CMV in lily plants and a simple reverse transcription loop-mediated isothermal amplification(RT-LAMP)to detect field CMV samples in lily bulbs. | Xiaorui Fan Yanan Du Youming Cai Yongchun Zhang Xiao Zhao Jieling Liang Dan Yang Qi Zhang Xiaoxia Zhang Wanjing Zhang Yan Xu Kai Zhao | 2019 | Acta Biochimica et Biophysica Sinica2019，51，2： | 0 |
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