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| 1 | Differential Inhibition of Nav1.7 and Neuropathic Pain by Hybridoma-Produced and Recombinant Monoclonal Antibodies that Target Nav1.7显示文摘The voltage-gated Na^+ channel subtype Nav1.7 is important for pain and itch in rodents and humans. We previously showed that a Nav1.7-targeting monoclonal antibody(SVmab) reduces Na+ currents and pain and itch responses in mice. Here, we investigated whether recombinant SVmab(rSVmab) binds to and blocks Nav1.7 similar to SVmab. ELISA tests revealed that SVmab was capable of binding to Nav1.7-expressing HEK293 cells,mouse DRG neurons, human nerve tissue, and the voltagesensor domain Ⅱ of Nav1.7. In contrast, rSVmab showed no or weak binding to Nav1.7 in these tests. Patch-clamp recordings showed that SVmab, but not rSVmab, markedly inhibited Na+ currents in Nav1.7-expressing HEK293 cells. Notably, electrical field stimulation increased the blocking activity of SVmab and rSVmab in Nav1.7-expressing HEK293 cells. SVmab was more effective than rSVmab in inhibiting paclitaxel-induced mechanical allodynia. SVmab also bound to human DRG neurons and inhibited their Na^+ currents. Finally, potential reasons for the differential efficacy of SVmab and rSVmab and future directions are discussed. | Sangsu Bang Jiho Yoo Xingrui Gong Di Liu Qingjian Han Xin Luo Wonseok Chang Gang Chen Sang-Taek Im Yong Ho Kim Judith A.Strong Ma-Zhong Zhang Jun-Ming Zhang Seok-Yong Lee Ru-Rong Ji | 2018 | Neuroscience Bulletin2018，34，1： | 2 |
| 2 | Design a Switch Wrapper for SNA On-Chip-Network 显示文摘 | Jiho Chang Jongsu Yi Jun Seong Kim et aI | 2006 | IEICE Transactions on Fundamentals of Electronics Communications and Computer Sciences2006，89，6： | 1 |
| 3 | Investigation of online community voluntary behavior using cognitive map显示文摘 | Inwon Kang Kun Chang Lee Sangjae Lee and Jiho Choi | 2007 | Computers in Human Behavior2007，23，1： | 1 |
| 4 | Investigation of the annealing effects on the structural and optical properties of sputtered ZnO thin films显示文摘 | Mina Jung Juyoung Lee Seungwhan Park Hongseung Kim Jiho Chang | 2005 | Journal of Crystal Growth2005，，3： | 1 |
| 5 | An early report: a modified porphyrin-linked metronidazole targeting intracellular Porphyromonas gingivalis in cultured oral epithelial cells显示文摘Porphyromonas gingivalis(P. gingivalis) has a strong association with the pathogenesis of periodontal disease. Recurrence of periodontal disease following therapy is attributed to numerous factors, and of growing interest is the potential problem of intracellular bacteria that are able to persist and multiply within the host cell, thereby facilitating relapse of infection. The effect of antibiotic therapy in controlling P. gingivalis is questionable. Accordingly, while metronidazole is very effective against anaerobic extracellular P. gingivalis by disrupting the DNA of anaerobic microbial cells, this antibiotic does not effectively penetrate into mammalian cells to inhibit intracellular bacteria. Therefore in the present study, a modified porphyrin-linked metronidazole adducts, developed in our laboratory, was used to kill intracellular P. gingivalis. A series of experiments were performed, including cytotoxicity assays and cellular uptake of adducts by flow cytometry coupled with live cell imaging analysis,P. gingivalis invasion and elimination assays, and the analysis of colocalization of P. gingivalis and porphyrin-linked metronidazole by confocal laser scanning microscopy. Findings indicated that P. gingivalis and porphyrin-linked metronidazole were colocalized in the cytoplasm, and this compound was able to kill P. gingivalis intracellular with a sufficient culture time.This is a novel antimicrobial approach in the elimination of P. gingivalis from the oral cavity. | Ping Ye Jiho Chang Lin Feng Foo Benjamin C-M Yap | 2017 | International Journal of Oral Science2017，9，3： | 1 |
| 6 | Plant-based,adjuvant-free,potent multivalent vaccines for avian influenza virus via Lactococcus surface display显示文摘Influenza epidemics frequently and unpredictably break out all over the world,and seriously affect the breeding industry and human activity.Inactivated and live attenuated viruses have been used as protective vaccines but exhibit high risks for biosafety.Subunit vaccines enjoy high biosafety and specificity but have a few weak points compared to inactivated virus or live attenuated virus vaccines,especially in low immunogenicity.In this study,we developed a new subunit vaccine platform for a potent,adjuvant-free,and multivalent vaccination.The ectodomains of hemagglutinins(HAs)of influenza viruses were expressed in plants as trimers(tHAs)to mimic their native forms.tHAs in plant extracts were directly used without purification for binding to inactivated Lactococcus(iLact)to produce iLact-tHAs,an antigen-carrying bacteria-like particle(BLP).tHAs BLP showed strong immune responses in mice and chickens without adjuvants.Moreover,simultaneous injection of two different antigens by two different formulas,t^(HAH5N6+H9N2) BLP or a combination of t^(HAH5N6) BLP and t^(HAH9N2) BLP,led to strong immune responses to both antigens.Based on these results,we propose combinations of plant-based antigen production and BLP-based delivery as a highly potent and cost-effective platform for multivalent vaccination for subunit vaccines. | Shi-Jian Song Gyeong-Im Shin Jinyong Noh Jiho Lee Deok-Hwan Kim Gyeongryul Ryu Gyeongik Ahn Hyungmin Jeon Hai-Ping Diao Youngmin Park Min Gab Kim Woe-Yeon Kim Young-Jin Kim Eun-Ju Sohn Chang Seon Song Inhwan Hwang | 2021 | Journal of Integrative Plant Biology2021，63，8： | 0 |
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