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您的检索式：作者名="Jinteng Cui"
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| 1 | Key microRNAs and target genes involved in regulating maturation in Lilium显示文摘Lilium is an ornamental bulb with a long juvenile stage,making its cultivation under natural conditions lengthy and costly.SQUAMOSA promoterbinding protein-like(SPL)transcription factors are related to plant growth and development,including phase transition.However,their role in phase transition in Lilium is not known.To explore the molecular mechanisms associated with the phase transition in Lilium,bulbs of Lilium Oriental Trumpet'Robina'were treated with lowered temperature to induce phase transition,and the small RNA and degradome were sequenced.A total of 161 miRNAs were identified as targets.Twenty-nine known miRNAs were differentially expressed,including 16 upregulated miRNAs and 13 down-regulated miRNAs.Lbr-miR156a was significantly down-regulated,and the target genes of Lbr-miR156a were identified as LbrSPL3 and LbrSPL16.Phylogenetic analysis showed that LbrSPL3 and LbrSPL16 had high homology with other plant SPLs.Subcellular localization and transcriptional activation experiments confirmed that LbrSPL3 and LbrSPL16 were mainly located in the nucleus and had transcriptional activity.The in situ hybridization results showed that the expression of LbrSPL3 and LbrSPL16 was increased following low-temperature treatment.Functional verification experiments of Arabidopsis transgenic plants showed that the overexpression of LbrSPL3 and LbrSPL16 could promote plant phase change,while the overexpression of Lbr-miR156a could inhibit this process.These results help elucidate the mechanism of phase transition regulation in Lilium and provide a reference for breeding research in other bulbous flowers. | Yao Chen Mengna Zhao Xiaoshan Wang Jinteng Cui Wei Ge Kezhong Zhang | 2022 | Ornamental Plant Research2022，2，1： | 0 |
| 2 | Pollination Methods and Embryo Rescue Techniques of Hybridization between Lilium Oriental Hybrids显示文摘The pollination methods and embryo rescue techniques of hybridization between Lilium Oriental hybrids were studied in this paper. The results showed that:( 1) in the combination of‘Justina'בShela',pollen tubes were observed to grow downward in styles and target to ovules in ovaries when normal stigma pollination method was used; pollen tubes were found to grow directionless to a different degree at stylar cuts and in ovaries when cut-style pollination method was used; the longer the styles were left,the more pollen tubes toward to ovules were observed and more plump seeds were produced; the maximum plump seeds were obtained via normal stigma pollination.( 2) Hybridization experiments were performed for the 56 combinations of Lilium Oriental hybrids with normal stigma pollination,and one combination generated no plump ovule and three combinations generated fewer than 10 plump ovules while the other 52 combinations generated plenty of plump ovules,which preliminarily indicated that normal stigma pollination method could be used as an universal pollination technique for hybridization between Lilium Oriental hybrids. The experiment results of different embryo rescue techniques showed that using cut-ovule inoculation about 70 d after pollination had a faster germination speed and a higher germination rate of hybrid embryo than normal ovule inoculation,and embryo rescue efficiency of the former method was obviously higher than ' embryo isolated inoculation' on 40 d after pollination. The research suggested that ' cut-ovule inoculation' about 70 d after pollination could be regarded as an universal embryo rescue technique for hybridization between Lilium Oriental hybrids. | Yang SHAO Weihua WEN Jinteng CUI Yuehui JIA Kezhong ZHANG | 2015 | Agricultural Biotechnology2015，4，3： | 0 |
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