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| 1 | Challenges and Development Prospects of Ultra-Long and Ultra-Deep Mountain Tunnels显示文摘1. Introduction Social development has led to the placement of high standards on ultra-long and ultra-deep mountain tunnels. Disasters may be encountered during the construction and maintenance of such mountain tunnels due to high geostress, high geotemperature, high hydraulic pressure, and special adverse strata, in addition to various other problems caused by engineering activities. To deal with uncertain geological conditions during mountain tunnel construction, comprehensive geological prediction, refined monitoring, and dynamic design and construction methods based on information technology should be adopted. For the operation and maintenance of ultra-long tunnels, the concepts of dynamic evacuation rescue, active protection, energy conservation, and environmental protection should be fully embodied in order to address significant problems related to ventilation, rescue situations, and energy consumption. Moreover, integrated construction and maintenance should be carried out to achieve digital sensing and intelligent maintenance. | Hehua Zhu Jinxiu Yan Wenhao Liang | 2019 | Engineering2019，5，3： | 16 |
| 2 | The Epitope Study on the SARS-CoV Nucleocapsid Protein显示文摘The nucleocapsid protein (N protein) has been found to be an antigenic protein in a number of coronaviruses. Whether the N protein in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is antigenic remains to be elucidated. Using Western blot and Enzyme-linked Immunosorbent Assay (ELISA),the recombinant N proteins and the synthesized peptides derived from the N protein were screened in sera from SARS patients. All patient sera in this study displayed strong positive immunoreactivities against the recombinant N proteins,whereas normal sera gave negative immunoresponses to these proteins, indicating that the N protein of SARS-CoV is an antigenic protein. Furthermore, the epitope sites in the N protein were determined by competition experiments, in which the recombinant proteins or the synthesized peptides competed against the SARS-CoV proteins to bind to the antibodies raised in SARS sera. One epitope site located at the C-terminus was confirmed as the most antigenic region in this protein. A detailed screening of peptide with ELISA demonstrated that the amino sequence from Codons 371 to 407 was the epitope site at the C-terminus of the N protein. Understanding of the epitope sites could be very significant for developing an effective diagnostic approach to SARS. | Shuting Li, Liang Lin, Hao Wang, Jianning Yin, Yan Ren, Zhe Zhao, Jie Wen, Cuiqi Zhou, Xumin Zhang, Xiaolei Li, Jingqiang Wang, Zhengfeng Zhou, Jinxiu Liu, Jianmin Shao, Tingting Lei, Jianqiu Fang, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 9 |
| 3 | Effects of environment lighting on the growth,photosynthesis,and quality of hydroponic lettuce in a plant factory显示文摘Leafy vegetable production under controlled environment using artificial lighting has many advantages over conventional greenhouses and open-field production.However,high initial investment and operation costs are restricting the wide application of this technology.In order to design an optimal artificial lighting environment for lettuce production,effects of different combinations of light intensity,photoperiod,and light quality on growth,quality,photosynthesis,and energy use efficiency of lettuce(Lactuca sativa L.cv Ziwei)were investigated under a closed plant factory.Lettuce transplants were grown under photosynthetic photon flux density(PPFD)at 150μmol/m^(2)·s,200μmol/m^(2)·s,250μmol/m^(2)·s,and 300μmol/m^(2)·s provided by fluorescent lamps(FL)with a red to blue ratio(R:B ratio)of 1.8 and light-emitting diode(LED)lamps with R:B ratio of 1.2 and 2.2,in combination with photoperiod of 12 and 16 h/d.In order to examine the“long term”photosynthetic characteristics,net photosynthetic rates of hydroponic lettuce leaves were continuously measured for 2 d(15^(th) and 16^(th) day after transplanting)before harvest.There was no difference in leaf fresh weight(FW)between PPFD of 250μmol/m^(2)·s and 300μmol/m^(2)·s with photoperiod of 16 h/d,regardless of light quality,and same results showed in contents of nitrate,soluble sugar,and vitamin C,respectively.The results of continuous measurements of net photosynthetic rate of lettuce leaves before harvest indicated that plants grown at PPFD of 250μmol/m^(2)·s had consistently higher compared to those grown at PPFD of 300μmol/m^(2)·s.Combining the results from growth,photosynthesis,quality,and energy consumption,it can be concluded that PPFD at 250μmol/m^(2)·s with photoperiod of 16 h/d under LED with R:B ratio of 2.2 is a suitable light environment for maximum growth and high quality of commercial lettuce(cv.Ziwei)production under indoor controlled environment. | Xin Zhang Dongxian He Genhua Niu Zhengnan Yan Jinxiu Song | 2018 | International Journal of Agricultural and Biological Engineering2018，11，2： | 8 |
| 4 | D-amino acid substitution enhances the stability of antimicrobial peptide polybia-CP显示文摘 | Fengjing Jia Jiayi Wang Jinxiu Peng Ping Zhao Ziqing Kong Kairong Wang Wenjin Yan Rui Wang | 2017 | Acta Biochimica et Biophysica Sinica2017，49，10： | 5 |
| 5 | Efficacy of Cytidine-5'-diphosp-bocholine Combined with Compound Anisodine in the Treatment of Early Optic Nerve Contusion显示文摘Purpose:To investigate the efficacy of anisodine combined with cytidine-5'-diphosp-bocholine (citicoline) in the treatment of early optic nerve contusion.Methods:A total of 33 subjects eligible for inclusion were selected from 105 patients clinically diagnosed with optic nerve contusion.These patients were subsequently divided into the control group (n =16) and the intervention group (n =17).In the control group,the participants received therapy consisting of glucocorticoids,mannitol,vasodilators and vitamin B.The patients in the intervention group additionally received anisodine in combination with citicoline.The visual acuity was graded on a scale from 0 to 8.Results:Prior to treatment,.the 25th,50th and 75th percentiles of visual acuity grade were 3,4 and 6.75 for the controls,and 3,4 and 6.5 for the patients in the intervention group (P=0.97).After treatment,the 25th,50th and 75th percentiles of visual acuity grade were 4,6 and 7.75 in the control group,and 7,7 and 8 in the intervention group (P=0.046).A significant difference was observed in both control (P=0.005) and intervention groups(P=0.001) when comparing presenting visual acuity before and after treatment.Conclusion:The combination of anisodine and citicoline with standard steroid and mannitol therapy appears to be effective in the treatment of early optic nerve contusion. | Wenhua Wang Yan Huang Jianli Zhang Jiahai Jiang Jinxiu Huang | 2012 | Eye Science2012，27，1： | 3 |
| 6 | Clinical Study on Interferon Treatment of Early Scarring in Filtering Bleb显示文摘Purpose:To investigate the effectiveness of needle revision combined with subconjuctival injection of interferon α-2b in reversing early scarring of filtering blebs following trabeculectomy surgery.Methods:Twenty-five glaucoma patients (31 eyes) who presented with scarred or encapsulated filtering bleb after glaucoma surgery underwent needle revision in combination with subconjuctival injection of interferon α-2b,and were followed for 12 months.Intraocular pressure (IOP) and filtering bleb morphology were observed post treatment.Results:.The mean time until scarring occurred was 21.0±7.4 days..The average time between recognition of bleb scarring and completion of needle revision was 2.2±0.8 days.The time interval between surgery and needle revision was inversely correlated with the time until needle revision (r=-0.694,P < 0.001).The mean IOPs before and after needle revision were 24.2±2.7 mmHg and 19.6±3.8 mmHg,respectively (t=5.916,P < 0.001)..At the 12-month follow-up visit,.18 eyes (58.1%).achieved complete success in IOP control,.and 6 eyes (19.4%) had conditional success..The overall success rate for needling was thus 77.4%.Subconjunctival hemorrhage was observed in 4 eyes during the needle revision procedure.Punctate staining was found in the corneal epithelium of 2 eyes.Shallow anterior chamber (Grade I or II) was identified in 5 eyes.Conclusion:Slit-lamp needle revision combined with subconjunctival injection of interferon α-2b may be efficacious in the treatment of early scarring of filtering blebs,easy and safe to perform,.and may be considered for more widespread application. | Wenhua Wang Jianli Zhang Yan Huang Jinxiu Huang Jiahai Jiang | 2011 | Eye Science2011，26，4： | 2 |
| 7 | Cu^2+ reduces hemolytic activity of the antimicrobial peptide HMPI and enhances its trypsin resistance显示文摘Nowadays,drug-resistant microbes are becoming a serious clinical problem threatening people's health and life.An timicrobial peptides(AMPs)are believed to be pote ntial alter natives of con-ventional antibiotics to combat the threat of drug-resistant microbes.However,the susceptibility of AMPs toward proteases is one of the major problems limiting their clinical use.In the present study,we reported the effect of Cu^2+on the bioactivity of AMP HMPI.We found that the addition of Cu^2+could improve the protease resistance of AMP HMPI without affecting its bioactivity.Notably,after the binding of Cu^2+with HMPI,the hemolytic activity of HMPI was greatly decreased.In additi on,our results also demonstrated that the addition of Cu^2+in creased the production of reactive oxygen species in the fun gal cells,which may be a suppleme nt for the antifungal activity of HMPI.In con elusion,the introduction of Cu^2+may provide an inorganic strategy to improve the stability and decrease the hemolytic activity of AMP HMPI,while maintaining its antifungal activity. | Jinxiu Peng Yang Yang Ping Zhao Shuai Qiu Fengjing Jia Jiayi Wang Xiaolei Liang Ahmed Shabbir Chaudhry Peihan Xu Wenjin Yan Zhaoqing Xu Kairong Wang | 2020 | Acta Biochimica et Biophysica Sinica2020，52，6： | 2 |
| 8 | Molybdenum Nanoscrews:A Novel Non-coinage-Metal Substrate for Surface-Enhanced Raman Scattering显示文摘For the first time, Mo nanoscrew was cultivated as a novel non-coinage-metal substrate for surface-enhanced Raman scattering(SERS). It was found that the nanoscrew is composed of many small screw threads stacking along its length direction with small separations. Under external light excitation, strong electromagnetic coupling was initiated within the gaps, and many hot-spots formed on the surface of the nanoscrew, which was confirmed by high-resolution scanning near-field optical microscope measurements and numerical simulations using finite element method. These hotspots are responsible for the observed SERS activity of the nanoscrews. Raman mapping characterizations further revealed the excellent reproducibility of the SERS activity. Our findings may pave the way for design of low-cost and stable SERS substrates. | Di An Yan Shen Jinxiu Wen Zebo Zheng Jun Chen Juncong She Huanjun Chen Shaozhi Deng Ningsheng Xu | 2017 | Nano-Micro Letters2017，9，1： | 1 |
| 9 | A Strategy for Searching Antigenic Regions in the SARS-CoV Spike Protein显示文摘In the face of the worldwide threat of severe acute respiratory syndrome (SARS)to human life, some of the most urgent challenges are to develop fast and accurate analytical methods for early diagnosis of this disease as well as to create a safe anti-viral vaccine for prevention. To these ends, we investigated the antigenicity of the spike protein (S protein), a major structural protein in the SARS-coronavirus (SARS-CoV). Based upon the theoretical analysis for hydrophobicity of the S protein, 18 peptides were synthesized. Using Enzyme-Linked Immunosorbent Assay (ELISA), these peptides were screened in the sera from SARS patients. According to these results, two fragments of the S gene were amplified by PCR and cloned into pET-32a. Both S fragments were expressed in the BL-21 strain and further purified with an affinity chromatography. These recombinant S fragments were confirmed to have positive cross-reactions with SARS sera, either by Western blot or by ELISA. Our results demonstrated that the potential epitope regions were located at Codons 469-882 in the S protein, and one epitope site was located at Codons 599-620. Identification of antigenic regions in the SARS-CoV S protein may be important for the functional studies of this virus or the development of clinical diagnosis. | Yan Ren, Zhengfeng Zhou, Jinxiu Liu, Liang Lin, Shuting Li, Hao Wang, Ji Xia, Zhe Zhao, Jie Wen, Cuiqi Zhou, Jingqiang Wang, Jianning Yin, Ningzhi Xu, and Siqi LiuBeijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China & Beijing Proteomics Institute, Beijing 101300, China. | 2003 | Genomics, Proteomics & Bioinformatics2003，1，3： | 1 |
| 10 | Newly recorded bloom-forming dinoflagellate Gymnodinium impudicum in Haizhou Bay,Yellow Sea,China显示文摘With the development of industrialization and aquaculture in Jiangsu and Shandong Provinces along the South Yellow Sea coast,China,eutrophication has greatly intensified in the region,resulting in frequent occurrence of diverse harmful algal blooms.An algal bloom formed by a chain-forming dinofl agellate species was recorded in the Haizhou Bay,South Yellow Sea,in September 2020.The causative species was isolated and studied in morphology,molecular phylogeny,pigment profile,presence of paralytic shellfish toxins,and acute toxicity.The loop-shaped apical groove running anticlockwise around the apex,the presence of peridinin as characteristic pigment,as well as a single phylogenic clade of 28S ribosomal DNA(100%posterior probability),defined this species as Gymnodinium impudicum,a non-toxic species that exhibited no obvious biotoxicity to the rotifer Brachionus plicatilis,the copepod Artemia salina,and the shrimp Neomysis awatschensis.Gymnodinium impudicum is typically distributed in coastal waters with high nitrate concentrations,where it reaches a maximum density of 2.6×10~5 cells/L.This is the first report of a G.impudicum bloom in the Yellow Sea;however,G.impudicum blooms may have been misidentified or underreported in Haizhou Bay due to the species morphological similarity with G.catenatum.A combination of multiple methods is recommended to accurately identify new algal bloom species. | Jinxiu WANG Fanzhou KONG Yunfeng WANG Nanjing JI Minjie SONG Zhangxi HU Zhuang NIU Chao LIU Xin WANG Yuanyuan SUN Rencheng YU Tian YAN | 2022 | Journal of Oceanology and Limnology2022，40，6： | 1 |
| 11 | Effects of Low Temperature and Poor Light Environments under LED Lighting on Quality Change of Pepper Seedlings during Storage显示文摘Storage and transportation of vegetable commercial seedlings becomes an important link along with the continuous expansion of planting areas of facility vegetables and vegetable seedling culture industry. In this study,pepper variety‘Nongda 24' was as the experimental material,and changes in the morphological and physiological indicators of plug seedlings in different low temperature( 6 and 11℃) and poor light environments were studied to find suitable storage environmental conditions. The results showed that pepper seedlings could be stored and transported for 14 days when storage temperature was 11℃ and light conditions such as light intensity and photoperiod were 30 μmol/( m^2·s)and 12 h/d by using a fluorescent lamp with R∶ B ratio of 1. 9,and 15 μmol/( m^2·s) and 24 h/d by using LED with R∶ B ratio of 1. 3,respectively. Moreover,the quality of pepper seedlings could be protected effectively. Compared to the fluorescent lamp,LED with high luminous efficiency and low energy consumption was suitable for the storage or long-distance transportation of pepper seedlings in low temperature and poor light environments. | Zhengnan YAN Dongxian HE Jinxiu SONG Yongsan CHENG Weifen DU | 2018 | Asian Agricultural Research2018，10，2： | 0 |
| 12 | PHASE DIAGRAMS OF KF-LiCI-BaCl_2 AND LiF-LiCI-BaCl_2 SYSTEMS显示文摘The phase diagrams of molten salt systems KF-LiCI-BaCl2 and LiCI-LiF-BaCl2 havebeen determined by visual polythermal method.The relationship between the change of cationradius of monovalent fluoride and the areas of primary crystal region in the phase diagrams isdiscussed. | YAN Licheng YANG Jinxiu ZHU Lei,XU Jingyan Shanghai University of Science andTechnology,Shanghai,ChinaFAN Jinxia Suzhou Teachers′ School,Suzhou,China YAN Licheng,Associate Professor,Dept.of Chemistry,Shanghai University of Science and Technology,Shanghai 201800,China | 1990 | Acta Metallurgica Sinica(English Letters)1990，3，11： | 0 |
| 13 | Image Denoising with Adaptive Weighted Graph Filtering显示文摘Graph filtering,which is founded on the theory of graph signal processing,is proved as a useful tool for image denoising.Most graph filtering methods focus on learning an ideal lowpass filter to remove noise,where clean images are restored from noisy ones by retaining the image components in low graph frequency bands.However,this lowpass filter has limited ability to separate the low-frequency noise from clean images such that it makes the denoising procedure less effective.To address this issue,we propose an adaptive weighted graph filtering(AWGF)method to replace the design of traditional ideal lowpass filter.In detail,we reassess the existing low-rank denoising method with adaptive regularizer learning(ARLLR)from the view of graph filtering.A shrinkage approach subsequently is presented on the graph frequency domain,where the components of noisy image are adaptively decreased in each band by calculating their component significances.As a result,it makes the proposed graph filtering more explainable and suitable for denoising.Meanwhile,we demonstrate a graph filter under the constraint of subspace representation is employed in the ARLLR method.Therefore,ARLLR can be treated as a special form of graph filtering.It not only enriches the theory of graph filtering,but also builds a bridge from the low-rank methods to the graph filtering methods.In the experiments,we perform the AWGF method with a graph filter generated by the classical graph Laplacian matrix.The results show our method can achieve a comparable denoising performance with several state-of-the-art denoising methods. | Ying Chen Yibin Tang Lin Zhou Yan Zhou Jinxiu Zhu Li Zhao | 2020 | Computers, Materials & Continua2020，，8： | 0 |
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