| 1 | Rescued virus from infectious cDNA clone of rabbit hemorrhagic disease virus is adapted to RK13 cells line显示文摘Based on the infectious full-length cDNA clone of rabbit hemorrhagic disease virus (RHDV), the in vitro transcripts are introduced into RK13 cells. 12 h later, CPE could be observed clearly, and virual antigen could also be detected by IFA. The titre of the recovered virus is 104.6/mL. Immune electron micro-scopic observation of the virus particles revealed that the particles were rotund with a diameter of about 30 nm. Besides, virus titre quantification obtained by qRT-PCR showed a correlation between time from infection and virus titre. All these results showed that we have recovered RHDV from RK13 cells by re-verse genetics technology successfully, and this would be very useful in studies of the antigenicity, virulence, pathogenesis, maturation and new type vaccines of RHDV. | LIU Guangqing NI Zheng YUN Tao ZHANG Yuying DU Qingyun SHENG Zutian LIANG Huali HUA Jionggang LI Shuangmao Chen Jianping | 2006 | Chinese Science Bulletin2006，51，14： | 9 |
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