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3篇 您的检索式:作者名="Jishun Tang"
    题名 作者 年代 出处 被引量
1Illite Crystallinity Mapping of Very Low Grade Metamorphism of Triassic Metapelites in the Zoigê Area,Western China显示文摘X-ray diffraction methods for estimating the metamorphic grade of diagenetic,anchizone and epizone in metapelites are reviewed and applied to samples from a 7000m+ borehole in western China and surface samples from the surrounding Zoigê area.Kübler's illite crystallinity(IC) measurements provide more consistent results than calculated values of percentage of illite in the I/S mixed layers and percentage of I/S mixed layers.Down-borehole IC values display a typical burial metamorphic relationship between stratigraphic level and IC.A method for preparing very low grade metamorphic maps is described,and isograds plotted on a regional geological map at selected values of IC,delineating a high temperature diagenetic zone,an anchizone,and an epizone.The map shows that IC values are controlled by stratigraphic level in the north of the study area(i.e.burial metamorphism),and proximity to an igneous intrusive body in the south(i.e.contact metamorphism).TANG Yan SANG Longkang YUAN Yanming YU Jishun ZHANG Yunpeng QI Xianmao YANG Yunlong 2012Acta Geologica Sinica(English Edition)2012,86,1:6
2Expression,structure and function analysis of the sperm-oocyte fusion genes Juno and Izumo1 in sheep(Ovis aries)显示文摘Background:JUNO and IZUMO1 are the first receptor-ligand protein pairs discovered to be essential for spermoocyte fusion;their interaction is indispensable for fertilization.Methods:PCR was used to clone the full-length DNA sequence of the Juno gene in sheep.The single nucleotide polymorphism(SNP)loci of Juno were genotyped by Sequenom MassARRAY®.PCR combined with rapid amplification of cDNA Ends were used to clone the full-length cDNA sequence of Juno and Izumo1.Reverse transcriptase-PCR(RT-PCR)and real time-quantitative-PCR(RT-qPCR)were used to analyze the genes’expression in tissues of sheep,and single cell RNA-seq was used to analyze the genes’expression in oocytes,granulosa cells and follicular theca of polytocous and monotocous Small Tail Han ewes.Bioinformatics was used to analyze advanced structure and phylogeny of JUNO and IZUMO1 proteins.Results:The full-length DNA sequence of the Juno gene in sheep was cloned and nine SNPs were screened.We found a significant association between the g.848253 C>A locus of Juno and litter size of Small Tail Han sheep(P<0.05).The full-length cDNA sequence of Juno and Izumo1 genes from Small Tail Han sheep were obtained.We found a new segment of the Izumo1 CDS consisting of 35 bp,and we confirmed the Izumo1 gene has 9 exons,not 8.RT-qPCR showed that Juno and Izumo1 genes were highly expressed in ovarian and testicular tissues,respectively(P<0.01).Single cell RNA-seq showed Juno was specifically expressed in oocytes,but not in granulosa cells or follicular theca,while Izumo1 displayed little to no expression in all three cell types.There was no difference in expression of the Juno gene in oocyte and ovarian tissue in sheep with different litter sizes,indicating expression of Juno is not related to litter size traits.Bioinformatic analysis revealed the g.848253 C>A locus of Juno results in a nonconservative missense point mutation leading to a change from Phe to Leu at position 219 in the amino acid sequence.Conclusions:For the first time,this study systematically analyzed the expression,structure and function of Juno and Izumo1 genes and their encoded proteins in Small Tail Han sheep,providing the basis for future studies of the regulatory mechanisms of Juno and Izumo1 genes.Wenping Hu Xinlong Dong Zhilong Tian Zhuangbiao Zhang Jishun Tang Benmeng Liang Qiuyue Liu Mingxing Chu 2021Journal of Animal Science and Biotechnology2021,12,3:3
3A New Lightweight Database Encryption and Security Scheme for Internet-of-Things显示文摘Internet-of-Things(IoT)extends the power of Internet and bring tremendous opportunity to academia and industry.However the security and data privacy challenges become major obstacles for its adoption and deployment.To address these issues,an encryption and security scheme is proposed for a lightweight database which is suitable for embedded systems with limited storage and computing resources.The scheme encrypts data are in both storage and used memory.So it can prevent sensitive data leakage from untrusted applications,zero-day-vulnerability and malicious attacks for the lightweight database.The prototype of the proposed scheme was presented and the feasibility and effectiveness was evaluated.The experimental results demonstrate the scheme is practical and effective.Jishun Liu Yan Zhang Zhengda Zhou Huabin Tang 2020国际计算机前沿大会会议论文集2020,,2:0
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