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您的检索式：作者名="Jose Alcami"
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| 1 | Evaluation of the Abbott LCx Quantitative Assay for Measurement of Human Immunodeficiency Virus RNA in Plasma显示文摘 | Carmen de Mendoza Jose Alcami Maria Saina | 2002 | Journal of Clinical Microbiology2002，40，4： | 1 |
| 2 | Nanostructured biohybrid material with wide-ranging antiviral action显示文摘Respiratory pathogens kill more people than any other infectious agent each year worldwide.Development of novel,economically friendly,sustainable,and highly efficient materials against viruses is a major challenge.Herein,we describe a nanostructured material composed of very small crystalline phosphate copper nanoparticles synthesized using a new biohybrid technology that employs a biological agent for its formation at room temperature in aqueous media.The evaluation of different enzymes in the final preparation of the nanomaterial or even in synthetic methods was performed.Biochemical characterization revealed the formation of Cu species in the protein network.The best biomaterial synthesized using a lipase called BioCuNPs showed excellent inhibition capacity against functional proteins of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2);for example,assent 3-chymotrypsin like protease(3CLpro)complete inhibition was achieved by using 5µg/mL,or acetone(ACE)–spike protein interaction was inhibited by more than 80%in the presence of 400µg/mL of BioCuNPs.Taking these in vitro results into account,an efficacy analysis against human coronavirus 229E(HCoV-E229)coronavirus was performed.A virus reduction of 99%was obtained in 5 min.Additionally,SARS-CoV-2 virus was tested to demonstrate high efficiency,with>99%inhibition in 15 min using 500 microgram of material.To determine the wide applicability of this nanohybrid against viruses,an evaluation was carried out against a non-enveloped virus such as Human Rhinovirus(HRV-14),obtaining a virus reduction of 99.9%in 5 min.Finally,the virucidal capacity against different bacteriophages was also evaluated,obtaining an excellent inhibition effect against PhageΦX174(99.999%reduction in 5 min). | Noelia Losada-Garcia Angela Vazquez-Calvo David Ortega-Alarcon Olga Abian Adrian Velazquez-Campoy Pilar Domingo-Calap Antonio Alcami Jose M.Palomo | 2023 | Nano Research2023，16，8： | 0 |
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