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| 1 | Apoptosis and its relationship with cell proliferation,p53,Waf1p21,bcl-2 and c-mycin esophageal carcinogenesis studied with a high-risk population in northern China显示文摘Apoptosisanditsrelationshipwithcelproliferation,p53,Waf1p21,bcl2andcmycinesophagealcarcinogenesisstudiedwithahighriskpopul... | WANG LiDong1, ZHOU Qi1, WEI JunPing1, YANG WanCai1, ZHAO Xin1, WANG LiXia1, ZOU Jian Xiang1, GAO ShanShan1, LI YongXin1 and YANG C.S.2Keywords esophageal neoplasms apoptosis precancerous lesions Waf1 p21 gene p53 | 1998 | World Journal of Gastroenterology1998，4，4： | 295 |
| 2 | Association of H.pylori infection with gastric carcinoma:a Meta analysis显示文摘AIM: To follow the principles of evidence based medicine to reach the integrated results of these studies.METHODS: Twenty-one papers of case-control studies were selected, including 11 on gastric cancer, 7 on precancerous lesion of stomach and 3 on lymphoma of stomach: Meta analysis was used to sum up the odds ratios (OR) of these studies.RESULTS: H. Pylori vsgastric cancer (intestinal and diffuse type): the odds ratio from the fixed effect model is 3.0016(95% Cl 2.4197-3.7234, P < 0.001 ). H. Pylori vs precancerous lesion of stomach: a random effect model was used to calculate the summary odds ratio and its value is 2.5635 (95% Cl: 1.8477-3.5566, P < 0.01). H. Pylori vs lymphoma of stomach: though the quantity of literature is too small to make Meta analysis, the data of these 3 studies show that lymphoma of stomach is highly associated with H. Pylori infections.CONCLUSION: Since it had been revealed that H. Pylori infection pre-exists in gastric carcinoma and precancerous lesions, the results of Meta analysis present a strong evidence to support the conclusion that H. Pylori infection is a risk factor for gastric carcinoma. | Fu-Bo Xue~1 Yong-Yong Xu~1 Yi Wan~1 Bo-Rong Pan~2 Jun Ren~2 Dai-Ming Fan~3 1 Department of Health Statistics,Department of2 Oncology3 Gastroenterology of XiJing Hospital,the Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2001 | World Journal of Gastroenterology2001，7，6： | 66 |
| 3 | The vegetation and climate change during Neocene and Early Quaternary in Jiuxi Basin, China显示文摘Sporopollen record in the Laojunmiao Section at Yumen in the Hexi Corridor foreland depression at the northern margin of the Tibetan Plateau revealed that during the period of 13.0―11.15 Ma the ecological environment of the Jiuxi Basin is characterized by steppe vegetation and a semi-moist climate. During 11.16―8.60 Ma prevailed forests of cypress and a still warmer, moister climate; steppe vegetation and dry climate began probably at about 8.6 Ma. Although aridification had been relaxed time and again during 8.40―6.93 Ma (forest-steppe, warm-semi-moist), 6.64―5.67 Ma (open-forest and steppe, warmer-semi-moist) and 5.42―4.96 Ma (steppe, semi-arid), the climate in the region became drier and drier in response to the fre- quent occurrence of aridity during 6.93―6.64 Ma (steppe, semi-arid), 5.67―5.42 Ma (de- sert-steppe, arid), 3.66―3.30 Ma (desert-steppe, arid) and 2.56―2.21 Ma (desert, arid). Perhaps the important findings of our study are the notable expansion of drought-enduring plants during 3.66―3.30 Ma and about 2.56 Ma and the replacement of vegetation by vast arid desert. | MA Yuzhen 1 , FANG Xiaomin 1,2 , LI Jijun 1 , WU Fuli 1 & ZHANG Jun 1 1. Key Laboratory of Western China’s Environmental Systems and College of Resources and Environment, Lanzhou University, Lanzhou 730000, China 2. State Key Laboratory of Loess and Quaternary Geology, Institute of Earth Environment, Chinese Academy of Sciences, Xi’an 710075, China | 2005 | Science China Earth Sciences2005，48，5： | 31 |
| 4 | The abnormal expression of retinoic acid receptor-β,p53 and Ki67 protein,n normal,premalignant and malignant esophageal tissues显示文摘AIM: Esophageal cancer remains a significant healthiproblem worldwide. It is important to investigate alterationsin expression of retinoic acid receptor-β, p53 and Ki67proteins in esophageal carcinogenesis.METHODS: To find biomarkers for early identification ofesophageal cancer, we analyzed the retinoic acid receptor-j3,p53 protein and the proliferation marker Ki67 in surgicalspecimens of normal, mildly, and severely dysplastic andmalignant esophageal tissues by in situ hybridization ofRNA and immunohistochemistry.RESULTS: RAR-β was expressed in 94.3 %(33/35) of normalmucosae, 67.8 %(19/28) of the mild, 58.1% (18/31) of thesevere ieaions and 53.2 % (116/218) of tumor samples. RAR-β mRNA was expressed in 62.7 % (42/67), 55.1% (43/78) and29.2 % (7/24) of well, moderated and poorly differentiatedSSCs. The p53 and Ki67 proteins were 5.9 % (2/34) of thenormal mucosa. P53 and Ki67 stained positively in 10.7 % (3/28) and 21.4 % (6/28) of mild dysplasia, and 51.6 %(16/31)and 58. 1% (18/31) of severely dysplasia respectively.Samples from esophageal cancer showed no higher levers ofp53 and Ki67 expression than seen in severely dysplasticlesions. There was significant difference of RAR-β、 p53 andKi67 expression between normal mucosa and dysplatictissue or esophageal cancer.CONCLUSION: Loss of RAR-β expression and acctnulation ofp53 and Ki67 proteins may serve as biomarkers for earlyidentification of esophageal cancer in the high-riskpopulations. | Min Xu Sheng-Zu Chen,Department of Nuclear Medcine,Cancer Hospital(Institute),Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 10002,1,China Yu-Lan Jin Jun Fu Hong Huang Ping Qu Hai-Mei Tian ZhaoYang Liu Wei Zhang,Central Laboratory for Tumor Biology,Cancer Hospital(Institute),Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 100021,China | 2002 | World Journal of Gastroenterology2002，8，2： | 34 |
| 5 | Studies on mechanism of Sialy Lewis-X antigen in liver metastases of human colorectal carcinoma显示文摘INTRODUCTIONSialyl Lewis-X antigen ,correlated with carcinoma, is a group of carbohydrate antigen containing oligosaccharide expressed of embryonic tisue and glycoproteins on cell surface of embryonic tissue[1].The SLeX antigen located on cell surface is synthesized principally by two enzymes ,al ,3fucosyltransfrease and a2, 3sialyctransferase.In adults ,SLeX antigen is expressed principally on the surfaces of granulocytic cells and some tumor cells . | Xiao Wei Li~1 Yan Qing Ding~1 Jun Jie Cai~1 Shao Qing Yang~2 Lian Bing An~3 Dong Fang Qiao~3 ~1Department of Pathology,Nanfang Hospital of the First Military Medical University,Guangzhou 510515,Guangdong Province,China ~2The Northern Hospital of PLA,Shenyang 110015,Liaoning Province,China ~3Department of Electronmicroscopy,First Military Medical University,Guangzhou 510515,Gangdong Province,ChinaDr.Xiao Wei Li graduated from the First Military Medical University with a MM degree in 1999.Physician in Charge of pathology,having 6 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 19 |
| 6 | Orthotopic transplantation model of human gastrointestinal cancer and detection of micrometastases显示文摘AIM To establish a relevant animal model ofhuman gastrointestinal cancer, which can beused for repetitive investigations, so as toimprove our understanding and management ofcarcinogenesis and cancer metastasis.METHODS Intact tissues of human colorectaland pancreatic cancers were transplanted innude mice. The biological characteristics of theoriginal and the corresponding transplantedtumors were investigated by HE staining, PASstaining and immunostaining. The metastases inthe livers and lungs of nude mice wereinvestigated by immunostaining withbiotinylated mab KL-1 and by RT-PCR using CK20specific primers.RESULTS There were totally 9 of 16 surgicalspecimens growing in nude mice subcutaneouslyand/.or orthotopically (4 of 6 colorectal and 5 of10 pancreatic cancer). Tumor cell content of thespecimens and freezing of tissue specimens areimportant factors influencing the growth oftransplanted tumor. In the group of fresh tumortissues with greater than 50% tumor cellcontent, the success rate of the transplantationwas 100% (3 cases of pancreatic cancer and 3cases of colorectal cancer). The orthotopicallytransplanted tumors resemble the original tumormorphologically and biologically, including TAAexpression such as CEA byimmunohistochemistry, and CEA level in theserum of mice. Ki-67 labeling index and theexpression of TAA especially K-ras, 17-lA andRA-96, are associated with the potential of tumorgrowth in nude mice. Micrometastases in thelungs and livers of tumor bearing mice can bedetected by immunostaining with biotinylatedmab KL-1 and CK20-specific RT-PCR.CONCLUSION An orthotopic transplantationmodel for human colon and pancreatic cancer innude mice has been set up. We have alsoestablished sensitive detection methods withCK-immunohistochemistry and CK20-RT-PCR tostudy xenotransplanted human cancer and itsmetastatic cancer cells in the liver and lung ofnude mice. This study may be helpful inunderstanding the mechanism of cancermetastasis and in developing new diagnosticmethods and therapeutic strategies formetastases including micrometastases. | Jun Hui Cui~1 Uwe Krueger~2 Doris Henne-Bruns~2 Bemd Kremer~2 Holger Kalthoff~2 ~1Department of General Surgery,First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,Zhejiang Province,China ~2Department of General Surgery,Christian-Albrechts-University,Kiel,GermanyDr.Jun Hui Cui graduated from Zhejiang Medical University in 1984,earned master degree in 1990,studied in the Surgical Department of Kiel University and worked in the Lab of Molecular Oncology of Kiel University from 1994-1997achieved M.D.from Kiel University.Germany,now associate professor of surgery,specialized in colorectal oncology.Adviser of graduated student for master degree,having 20 publications published in key Chinese or English journals. | 2001 | World Journal of Gastroenterology2001，7，3： | 19 |
| 7 | 新疆望峰金矿成矿流体研究及其成因意义显示文摘新疆望峰金矿,产于中天山结晶基底中的韧性剪切带内。围岩蚀变和流体包裹体研究表明,成矿过程包括了3个阶段,从早到晚有石英黄铁矿阶段、多金属硫化物阶段和石英碳酸盐阶段,温度分别集中在360~520℃、240~360℃和120~240℃;矿化以第Ⅱ阶段为主。成矿流体的δ18O值变化于1.15‰~2.70‰之间,属于变质热液与大气降水热液的混合流体。包裹体成分表明成矿流体来源较浅,但有利于金矿化;CO2/H2O比值多大于0.026,也有利于成矿。而包裹体捕获压力表明成矿作用发生在5.6~1.2km深度范围,主要发生在1.9km左右;矿化过程与地壳抬升伸展的过程一致,自然亦与碰撞造山体制的矿化规律一致。因此,古生代末期的碰撞造山作用导致了望峰金矿的形成,冰达坂断裂两侧是寻找望峰式金矿的有利地带。石英红外光谱分析表明,CO2光密度(D2)及其与H2O光密度(D1)的比值(D2/D1)普遍较高,指示矿化较强;D2>0.55和D2/D10.26是该区富金样品的标志。 | Chen Yanjing 1) Li Xin 1) Jing Jun 2) Gao Xiuli 1) Chen Huayong 1) Wu Xidan 2) Wu Dehua 2) ( 1) Department of Geology, Peking University 2)Team 15 of Gold Headquarters, MMIC, Urumqi ) | 1998 | 地球学报(中国地质科学院院报)1998，19，2： | 18 |
| 8 | Carcinogenic potential of duodenal reflux juice from patients with long-standing postgastrectomy显示文摘AIM To determine, whether study on thecarcinogenic potential of reflux juice frompatients with remote gastrectomy could clarifythe inherent relationship between duodenalreflux and gastric stump cancer.METHODS A total of 37 reflux juice samples(13 Billroth T, 24 Billroth Ⅱ ) were employed inthe present study. A two- stage transformationassay using BALB/c 3T3 cells was carried out totest the initiating or promoting activity of thesesamples.RESULTS Two of 18 (11.1%) reflux samplesexerted initiating activities, whereas 9/19(47.4%) samples enhanced the MNNG-initiafingcell transformation, suggesting the duodenalreflux juice might more frequently possess thetumor-promoter activity (P = 0. 029). In addition,there was no difference in initiating activities ofthe samples irrespective of surgical procedures(P=0.488), while Billroth Ⅱ samples exhibitedstronger tumor-promoter activity than Billroth Ⅰsamples (P = 0.027). Furthermore, the promoteractivities were well correlated with thehistological changes of the stomas (rs = 0.625,P = 0.004), but neither their cytotoxicities norinitiating activities had this correlation( Probabilities were 0.523 and 0.085,respectively).CONCLUSION The duodenal reflux juice frompatients with remote postgastrectomy did havecarcinogenic potential, and suggested thattumor-promoting activity should principallyaccount for the high incidence of gastric cancerin gastrectomy patients. In contrast, it isdifficult to explain the high stump-cancerincidence with the 'N-nitroso compounds'theory-a popular theory for the intact stomachcarcinogenesis, and it seemed to be justified tofocus chemoprevention of this cancer on thetumor-promoting potential of reflux juice. | Zhe Fu Ma~1 Zhong Yu Wang~1 Jun Ran Zhang~2 Peng Gong~1 Hai Long Chen~1 ~1Departrnent of General Surgery,First Hospital,Dalian Medical University,Dalian 116011,China ~2Department of Radiation Oncology,Massachusetts General Hospital and Harvard Medical School,Charlestown,MA 02129,USADr.Zhe Fu Ma graduated from Shanxi Medical College in 1993,nowPh.D.,M.D.in Department of Surgery,Dalian Medical University,majoring gastroenteric cancer,having 4 papers published.Correspondence to:Dr.Zhe Fu Ma.Department of General Surgery,First Hospital,Dalian Medical University,Dalian 116011,China Tel.0086-411-4720334 | 2001 | World Journal of Gastroenterology2001，7，3： | 9 |
| 9 | Construction of retroviral vectors to induce a strong expression of human class Ⅰ interferon gene in human hepatocellular carcinoma cells in vitro显示文摘ConstructionofretroviralvectorstoinduceastrongexpresionofhumanclasⅠinterferongeneinhumanhepatocelularcarcinomacelsinvitroCAOG... | CAO Guang Wen 1, GAO Jun 1, DU Ping 1, QI Zhong Tian 1 and KONG Xian Tao 2 | 1997 | World Journal of Gastroenterology1997，3，3： | 9 |
| 10 | The results and prognosis of different treatment modalities for solitary metastatic lung tumor from nasopharyngeal carcinoma:a retrospective study of 105 cases显示文摘Background and Objective:Nasopharyngeal carcinoma (NPC) is known for its propensity for distant metastases.Lung metastasis is one of the most important causes of death for patients with NPC.Solitary metastatic lung tumor from NPC is a distinctive group associated with a better survival.This study was to find a more effective treatment modality and prognostic factors for the group.Methods:Clinical data of 105 cases of solitary metastatic lung tumor from NPC were retrospectively analyzed.Survival rate was calculated by the Kaplan-Meier method.The difference of survival between the patients treated by different modalities was evaluated by the log-rank test.The Cox univariate and multivariate analyses of gender, age, pathologic type, stage, adjuvant chemotherapy, evaluation of treatment for NPC, disease-free interval, size of metastatic tumor, pulmonary hilar and/or mediastinal lymph node metastasis, treatment modalities, recurrent distant metastases and/or relapse of NPC were conducted.Results:The local control rate was 53.8% in chemotherapy group, 88.0% in radiotherapy +/-chemotherapy group, and 96.4% in operation +/-chemotherapy group (P<0.01).The most promising progression-free survival (PFS) and overall survival (OS) were obtained with operation +/-chemotherapy and followed by radiotherapy +/-chemotherapy.Both of them showed much better efficacy than chemotherapy (P<0.001).The Cox multivariate analysis showed that recurrent distant metastases and/or relapse of NPC affected the survival (OR=2.087, 95% CI=1.277-3.410, P=0.003).The T stage of NPC, size of metastatic tumor, hilar and/or mediastinal lymph node metastasis, and the treatment modality were independent prognostic factors.Conclusions:Operation +/-chemotherapy and radiotherapy +/-chemotherapy are better treatment of solitary metastatic lung tumor from NPC, which could improve the local control and prolong the PFS and OS.Chemotherapy is recommended for patients with higher T stage of NPC, size of metastatic tumor ≥3 cm, pulmonary hilar and/or mediastinal lymph node metastasis. | Jun Ma1,2, Zhe-Sheng Wen1,2, Peng Lin1,2, Xin Wang1,2, Fang-Yun Xie1,3 1 State Key Laboratory of Oncology in South China, Guangzhou, Guangdong 510060, P.R.China 2 Department of Thoracic Oncology, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, P.R.China 3 Department of Radiation Oncology, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, P.R.China Department of Thoracic Surgery, Shanxi Provincial People’s Hospital, Taiyuan, Shanxi 030012, P.R.China | 2010 | Chinese Journal of Cancer2010，29，9： | 9 |
| 11 | Clinical application of serial operations with preserving spleen显示文摘AIM: To evaluate the clinical application of serial operations with preservation of spleen.METHODS: Serial operations with preserving spleen were performed on 211 cases in our hospital from 1980 to 2000.The patient's age ranged from 13 to 56 years, averaging 3years. Diseases included splenic injury in 171 cases, portal hypertension in 9 cases, splenic cyst in 10 cases, and the lesion of pancreatic body and tail in 21 cases.RESULTS: All the cases were cured, and 129 patients were followedup from 3 months to 3 years with the leukocyte phagocytosis test, detection of immunoglubin, CT, 99mTc scanning and ultrasonogrsphy. The results were satisfactory.CONCLUSION: The operations with preserving spleen were safe, feasible, and worth of clinical application. | Hong-Chi Jiang~1 Bei Sun~1 Hai-Quan Qiao~1 Jun Xu~1 Da-Xun Piao~1 Hang Yin~2 1 Department of General Surgery,First Clinical Hospital,Harbin Medical University,Harbin 150001,China2 Department of General Surgery,Heilongjiang Provincial Hospital,Harbin 150001,China | 2001 | World Journal of Gastroenterology2001，7，6： | 8 |
| 12 | Consequence alimentary reconstruction in nutritional status after total gastrectomy for gastric cancer显示文摘NTODUCTIONToinvestigatethenutritionalconsequencesofgastroentericreconstructioninpatientswithgastriccanceraftertotalgastrecto... | WU Yin Ai 1, LU Bin 1, LIU Jun 1, LI Jiang 1, CHEN Jiang Rong 2 and HU Shi Xiong 1 | 1999 | World Journal of Gastroenterology1999，5，1： | 8 |
| 13 | Polymorphisms of three gene-derived STS on pig chromosome 13q41 are associated with susceptibility to enterotoxigenic Escherichia coli F4ab/ac in pigs显示文摘Neonatal diarrhea caused by enterotoxigenic Escherichia coli(ETEC)F4 is a common and serious disease,resulting in significant economical loss in the pig industry.The locus encoding ETEC F4 receptor has been mapped to pig chromosome(SSC)13q41,and one of the most significantly linked markers is S0075.In this study,we selected three genes including SLC12A8,MYLK and KPNA1 from a chromosomal region flanking S0075 on SSC13 to develop pig specific sequence tagged sites(STS). Seven single nucleotide polymorphisms were identified in the three pig STS using DNA of four full-sib susceptible and resistant animals in a White Duroc×Erhualian intercross.All grandparents,parents and 755 offspring in the intercross were genotyped for three polymorphisms,including SLC12A8 g.159A>G,MYLK g.1673A>G and KPNA1 g.306A>G.Family-based transmission disequilibrium test(TDT) revealed that all polymorphisms and the corresponding haplotypes are significantly associated with ETEC F4ab/ac(especially F4ac)brush border adhesion phenotypes,indicating that these polymor- phism are in linkage disequlibrium with causal mutation(s)of the gene encoding ETEC F4ab/ac receptor. Our results strengthen the evidence for the involvement of SSC13q41 in high acquiring risk of ETEC F4ab/ac infection,and provide novel polymorphic markers for fine mapping of the ETEC F4ab/ac receptor locus. | HUANG Xiang 1 ,REN Jun 1 ,YAN XueMing 1,2 ,PENG QiuLing 1 ,TANG Huan 1 ,ZHANG Bo 1 ,JI HuaYuan 1 , YANG ShuJin1&HUANG LuSheng 1 1Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China,Jiangxi Agricultural University, Nanchang 330045,China 2Department of Biology,Nanchang University of Science and Technology,Nanchang 330038,China | 2008 | Science China(Life Sciences)2008，51，7： | 8 |
| 14 | Comparative studies on epithelial lesions at gastric cardia and pyloric antrum in subjects from a high incidence area for esophageal cancer in Henan,China显示文摘ComparativestudiesonepitheliallesionsatgastriccardiaandpyloricantruminsubjectsfromahighincidenceareaforesophagealcancerinHena... | GAO ShanShan1, ZHOU Qi1, LI YongXin1, BAI YongMin1, ZHENG ZuoYu1, ZOU JianXiang1, LIU Gang1, FAN ZongMin1, QI YiJun1, ZHAO Xin1 and WANG LiDong1,2Keywords esophageal neoplasms stomach neoplasms gastric ca | 1998 | World Journal of Gastroenterology1998，4，4： | 8 |
| 15 | A novel ubiquitin carboxyl terminal hydroase is involved in toad oocyte maturation显示文摘p28, a 28kD protein from toad (Bufo bufo gargarizans) oocytes, was identified by using p13suc1-agaroseaffinity chromatography. Sequence homology analysis of the full-length cDNA of p28 (Gene Bank accessionnumber: AF 314091) indicated that it encodes a protein containing 224 amino-acids with about 55% iden-tities and more than 70% positives to human, rat or mouse UCH-L1, and contains homological functionaldomains of UCH family. Anti-p28 monoclonal antibody, on injecting into the oocytes, could inhibit theprogesterone-induced resumption of meiotic division in a dose-dependent manner. The recombinant proteinp28 showed similar SDS/PAGE behaviors to the native one, and promoted ubiquitin ethyl ester hydrolysis,a classical catalytic reaction for ubiquitin carboxyl terminai hydrolases (UCHs). The results in this paperreveal that a novel protein, p28, exists in the toad oocytes, is a UCH L1 homolog, was engaged in theprocess of progesterone-induced oocyte maturation possibly through an involvement in protein turnover anddegradation. | ZHAO GUI SUN1,2,3,*, WEI HUA KONG2,*, YAN JUN ZHANG2, SHAN YAN1, JI NING LU1, ZHENG GU1, FENG LIN1, JIA KE TSO1,**1 National Laboratory of Contraceptives and Devices Research, Shanghai Institute of Planned ParenthoodResearch, Shanghai 200032, China2 Colle | 2002 | Cell Research2002，12，4： | 8 |
| 16 | BRIF and CARIF progress显示文摘China Institute of Atomic Energy (CIAE) is currently constructing Beijing rare ion beam facility (BRIF) and is proposing China advanced rare ion beam facility (CARIF). This paper is aiming at introducing the progress of BRIF project and the con ceptual design CARIF. The ISOL type facility BRIF under construction is composed of a 100 MeV 300 ?A proton cyclotron, an ISOL with mass resolution of 20000, and a super-conducting LINAC of 2 MeV/q, and will be commissioned in 2013. CARIF facility proposed is planned to use both ISOL and PF techniques. It is based on a China advanced research reactor CARR that was critical, with ISOL separation of fission fragment, post acceleration to 150 MeV/u, and fragmentation of neu tron-rich fission fragment beam like 132Sn. Such unique combination will allow CARIF to deliver beam intensity better than the best world facilities by more than one order of magnitude. | LIU WeiPing, LI ZhiHong, BAI XiXiang, WANG YouBao, GUO Bing, PENG ChaoHua, YANG Yi, SU Jun, CUI BaoQun, ZHOU ShuHua, ZHU ShengYun, XIA HaiHong, GUAN XiaLing, ZENG Sheng, ZHANG HuanQiao, CHEN YongShou, TANG HongQing, HUANG Li & FENG BeiYuan China Institute of Atomic Energy, P. O. Box 275(1), Beijing 102413, China | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，S1： | 8 |
| 17 | Mechanism of exogenous nucleic acids and their precursors improving the repair of intestinal epithelium after 7-irradiation in mice显示文摘AIM To clone expressed genes associated withrepair of irradiation-damaged mice intestinalgland cells treated by small intestinal RNA,andto explore the molecular mechanism ofexogenous nucleic acids improving repair ofintestinal crypt.METHODS The animal mode of test group andcontrol group was established,forty-five micebeing irradiated by γ ray were treated with smallintestinal RNA as test group,forty mice beingirradiated by γ ray were treated withphysiological saline as control group,five micewithout irradiation were used as normal control,their jejunal specimens were collectedrespectively at 6h,12h,24h,4d and 8d afterirradiation.Then by using LD-PCR based onsubtractive hybridization,these gene fragmentsdifferentially expressed between test group andcontrol group were obtained,and then werecloned into T vectors as well as beingsequenced.Obtained sequences were screenedagainst.GeneBank,if being new sequences,they were submitted to GeneBank.RESULTS Ninety clones were associated withrepair of irradiation-damaged intestinal glandcells treated by intestinal RNA.These clonesfrom test group of 6h,12h,24h,4d and 8dwere respectively 18,22,25,13,12.By screening against GeneBank,18 of which werenew sequences,the others were dramaticallysimilar to the known sequences,mainly similarto hsp,Nmi,Dutt1,alkaline phosphatase,homeobox,anti-CEA ScFv antibody,arginine/serine kinase and BMP-4,repA.Eighteen genefragments were new sequences,their acceptnumbers in GeneBank were respectivelyAF240164-AF240181.CONCLUSION Ninety clones were obtained tobe associated with repair of irradiation-damagedmice intestinal gland cells treated by smallintestinal RNA,which may be related toabnormal expression of genes and matchedproteins of hsp,Nmi,Duttl,Na,K-ATPase,alkalineph-osphatase,glkA,single strandedreplicative centromeric gene as well as 18 newsequences. | Da Xiang Cui~1 Guei Ying Zeng~2 Feng Wang~1 Jun Rong Xu~1 Dong Qing Ren~2 Yan Hai Guo~1 Fu Rong Tian~2 Xiao Jun Yan~1 Yu Hou~1 Cheng Zhi Su~1 1 Institute of Genetic Diagnosis of the Fourth Military Medical University,Xi’an 710032,China 2 Department of Irradiation Medicine of the Fourth Military Medical University,Xi’an 710032,China | 2000 | World Journal of Gastroenterology2000，6，5： | 8 |
| 18 | SALVIANOLIC ACID H,A NEW DEPSIDE FROM SALVIA CAVALERIEI VAR.SIMPLICIFOLIA显示文摘A new depside named salvianolic acid H was isolated from the aqueous extract of Salviacavaleriei vat.simplicifolia,along with salvianolic acid A,B,C,isosalvianolic acid C,lithospermic acid,rosmarinic acid,R-(+)-β-(3,4-dihydroxyphenyl)lacticacid and protocatechualdehyde.Rosmarinic acid wasobtained as the major component. | Hai Jun ZHANG Lian Niang LI Institute of Materia Medica,Chinese Academy of Medical Sciences 1 Xian Nong Tan Street,Beijing 100050,China | 1993 | Chinese Chemical Letters1993，4，6： | 7 |
| 19 | Evaluation of Six Methods for Estimating Synonymous and Non-synonymous Substitution Rates显示文摘Methods for estimating synonymous and nonsynonymous substitution rates among protein-coding sequences adopt different mutation (substitution) models with sub- tle yet significant differences, which lead to different estimates of evolutionary information. Little attention has been devoted to the comparison of methods for obtaining reliable estimates since the amount of sequence variations within tar- geted datasets is always unpredictable. To our knowledge, there is little informa- tion available in literature about evaluation of these different methods. In this study, we compared six widely used methods and provided with evaluation results using simulated sequences. The results indicate that incorporating sequence fea- tures (such as transition/transversion bias and nucleotide/codon frequency bias) into methods could yield better performance. We recommend that conclusions related to or derived from Ka and Ks analyses should not be readily drawn only according to results from one method. | Zhang Zhang1,2,3 and Jun Yu1,2,4* 1 Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China 2 Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China 3Graduate School, Chinese Academy of Sciences, Beijing 100039, China 4James D. Watson Institute of Genome Sciences, Zhejiang University, Hangzhou 310007, China. | 2006 | Genomics, Proteomics & Bioinformatics2006，4，3： | 7 |
| 20 | Effect of Qinling granule in treatment of 102 patients with chronic hepatitis B显示文摘EfectofQinlinggranuleintreatmentof102patientswithchronichepatitisBHUANGZhengMing1,YANGXinBo1,CAOWenBin1,ZHOUYiJun2andLULi... | HUANG Zheng Ming 1, YANG Xin Bo 1, CAO Wen Bin 1, ZHOU Yi Jun 2 and LU Li Yan 2 1Department of Pharmacology, Chinese PLA Beijing Medical School, Beijing 100071, China 2Chinese PLA 184 Hospital, Yingtan 350000, Jiangxi Province, Ch | 1998 | World Journal of Gastroenterology1998，4，1： | 7 |
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