| 4 | VEGFR2 inhibition hampers breast cancer cell proliferation via enhanced mitochondrial biogenesis显示文摘Objective:Vascular endothelial growth factor(VEGF),apart from its predominant roles in angiogenesis,can enhance cancer cell proliferation,but its mechanisms remain elusive.The purpose of the present study was therefore to identify how VEGF regulates cancer cell proliferation.Methods:VEGF effects on cancer cell proliferation were investigated with the VEGF receptor 2 inhibitor,Ki8751,and the breast cancer cell lines,MCF-7 and MDA-MB-231,using flow cytometry,mass spectrometry,immunoblotting,and confocal microscopy.Data were analyzed using one-way analysis of variance followed by Tukey’s multiple comparison test.Results:VEGF blockade by Ki8751 significantly reduced cancer cell proliferation,and enhanced breast cancer cell apoptosis.Mass spectrometric analyses revealed that Ki8751 treatment significantly upregulated the expression of mitochondrial proteins,suggesting the involvement of mitochondrial biogenesis.Confocal microscopy and flow cytometric analyses showed that Ki8751 treatment robustly increased the mitochondrial masses of both cancer cells,induced endomitosis,and arrested cancer cells in the high aneuploid phase.VEGFR2 knockdown by sh RNAs showed similar effects to those of Ki8751,confirming the specificity of Ki8751 treatment.Enhanced mitochondrial biogenesis increased mitochondrial oxidative phosphorylation and stimulated reactive oxygen species(ROS)production,which induced cancer cell apoptosis.Furthermore,Ki8751 treatment downregulated the phosphorylation of Akt and PGC1α,and translocated PGC1αinto the nucleus.The PGC1αalterations increased mitochondrial transcription factor A(TFAM)expression and subsequently increased mitochondrial biogenesis.Conclusions:VEGF enhances cancer cell proliferation by decreasing Akt-PGC1α-TFAM signaling-mediated mitochondrial biogenesis,ROS production,and cell apoptosis.These findings suggested the anticancer potential of Ki8751 via increased mitochondrial biogenesis and ROS production. | Hao Ni Min Guo Xuepei Zhang Lei Jiang Shuai Tan Juan Yuan Huanhuan L Cui Yanan Min Junhao Zhang Susanne Schlisio Chunhong Ma Wangjun Liao Monica Nister Chunlin Chen Shuijie Li Nailin Li | 2021 | Cancer Biology & Medicine2021，18，1： | 0 |
| 5 | Electrochemical behaviors of anode materials and their performance for bauxite desulfurization显示文摘Pyrite inside bauxite could be oxidized into soluble S-containing ions by electrolysis, and thus achieving bauxite desulfurization by using filtration. However, S-containing ions in electrolyte had some corrosion effects on electrode, especially for anode. In this work, six kinds of traditional materials were selected as anode, and their corrosion behaviors were examined by using electrochemistry characterization. Tafel and CV curves from simulating electrolyte suggested that their corrosion potentials were in the following order: Ni﹥C﹥SS﹥Fe﹥Cu﹥Pb–Ag. As expected, the desulfurization ratio and cell voltage from bauxite electrolysis were in the following order respectively: Cu﹥Ni﹥Fe﹥SS﹥C﹥Pb–Ag and Ni﹥Fe﹥SS﹥Cu﹥C﹥Pb–Ag. Finally, Ni was proposed a kind of excellent electrode material for bauxite desulfurization from electrolysis. | Yingnan Hu Aijing Lü Mingyong Wang Dong Wang Junhao Liu Zhi Wang Xuzhong Gong | 2019 | Chinese Journal of Chemical Engineering2019，27，4： | 0 |
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