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您的检索式：作者名="Junkang Qiu"
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| 1 | Affinity maturation of anti-TNF-alpha scFv with somatic hypermutation in non-B cells显示文摘Activation-induced cytidine deaminase(AID)is required for the generation of antibody diversity through initiat-ing both somatic hypermutation(SHM)and class switch recombination.A few research groups have success-fully used the feature of AID for generating mutant li-braries in directed evolution of target proteins in B cells in vitro.B cells,cultured in suspension,are not con-venient for transfection and cloning.In this study,we established an AID-based mutant accumulation and sorting system in adherent human cells.Mouse AID gene was first transfected into the human non-small cell lung carcinoma H1299 cells,and a stable cell clone(H1299-AID)was selected.Afterwards,anti-hTNF-αscFv(ATscFv)was transfected into H1299-AID cells and ATscFv was displayed on the surface of H1299-AID cells.By 4-round amplification/flow cytometric sorting for cells with the highest affinities to hTNF-alpha,two ATscFv mutant gene clones were isolated.Compared with the wild type ATscFv,the two mutants were much more efficient in neutralizing cytotoxicity of hTNF-alpha.The results indicate that directed evolution by somatic hypermutation can be carried out in adherent non-B cells,which makes directed evolution in mammalian cells easier and more efficient. | Shaopeng Chen Junkang Qiu Chuan Chen Chunchun Liu Yuheng Liu Lili An Junying Jia Jie Tang Lijun Wu Haiying Hang | 2012 | Protein & Cell2012，3，6： | 0 |
| 2 | Piezoelectric nanofoams with the interlaced ultrathin graphene confining Zn–N–C dipoles for efficient piezocatalytic H_(2) evolution under low-frequency vibration显示文摘Unique nanofoams consisting of interweaved ultrathin graphene confining Zn–N–C dipoles (ZnNG) are constructed via calcination of Zn-coordinated precursor.Due to the introduction of local polar Zn–N–C configurations,with hypersensitivity for mechanical stress,the piezoelectricity is created on the nonpiezoelectric graphene,and the hierarchical ZnNG exhibits obvious piezocatalytic activity of water splitting for H_(2) production even under mild agitation.The corresponding rate of H_(2) production is about 14.65 μmol g^(-1)h^(-1).It triggers a breakthrough in piezocatalytic H_(2) evolution under low-frequency vibration,and takes a significant step forward for piezocatalysis towards practical applications.Furthermore,the presented concept of confining atomic polar configuration for engineering piezoelectricity would open up new horizon for constructing new-type piezoelectrics based on both piezoelectric and nonpiezoelectric materials. | Penghui Hu Yan Xu Yanhua Lei Jie Yuan Rui Lei Rong Hu Junkang Chen Difa Xu Shiying Zhang Ping Liu Xiangchao Zhang Xiaoqing Qiu Wenhui Feng | 2022 | Journal of Energy Chemistry2022，31，6： | 0 |
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