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| 1 | Chinese Multidisciplinary Expert Consensus on the Diagnosis and Treatment of Hyperuricemia and Related Diseases显示文摘 | Zou, He-Jian Wu, Hu-Sheng Zhou, Jing-Guo Zeng, Xue-Jun Dai, Lie Wu, Hua-Xiang Zhu, Xiao-Xia Mei, Chang-Lin Hao, Chuan-Ming Chen, Nan Liu, Bi-Cheng Chen, Jiang-Hua Yang, Li Nie, Jing Yu, Chen Peng, Ai Yu, Sheng-Qiang Li, Lin Ge, Jun-Bo Huo, Yong Zhang, Shu-Yang Chen, Yun-Dai Dong, Yu-Gang Liang, Chun Dai, Yu-Xiang Gao, Xin Li, Chang-Gui Zhao, Jia-Jun Chen, Hai-Bing Cheng, Zhi-Feng Lin, Huan-Dong Guan, Yang-Tai Wang, Kai Luo, Ben-Yan Dai, Ruo-Lian Jiang, Quan Xue, Luan Liang, Chao-Chao Chen, Ming Fan, Song | 2017 | Chinese Medical Journal2017，，20： | 30 |
| 2 | Evaluation of the Effects of Standard Rescue Procedure on Severe Trauma Treatment in China显示文摘 | Xiao-Feng Yin Tian-Bing Wang Pei-Xun Zhang Yu-Hui Kou Dian-Ying Zhang Kai Yu De-Cheng Lyu Mao-Zheng Liu Dong-Sheng Zhou Peng Zhang Jue-Hua Jing Wei-Wei Ge Li-Ying Cao Guo-Sheng Wang Shao-Jie Deng Weng-Hua Liu Mao Zhang Yong-An Xu Kun Zhang Bing Li Wei Wang Zhong-Li Gao Cheng-La Yi Bao-Guo Jiang | 2015 | Chinese Medical Journal2015，，10： | 18 |
| 3 | A Multicenter, Randomized, Double-Blind, and Placebo-Controlled Study of the Effects of Tongxinluo Capsules in Acute Coronary Syndrome Patients with High On-Treatment Platelet Reactivity显示文摘 | Lei Zhang Yi Li Bai-Song Yang Lu Li Xiao-Zeng Wang Mei-Ling Ge Quan-Min Jing Ying-Yan Ma Geng Wang Hai-Wei Liu Xin Zhao Bin Wang Kai Xu Ya-Ling Han | 2018 | Chinese Medical Journal2018，，5： | 16 |
| 4 | Experimental investigation of shale imbibition capacity and the factors influencing loss of hydraulic fracturing fluids显示文摘Spontaneous imbibition of water-based fracturing fluids into the shale matrix is considered to be the main mechanism responsible for the high volume of water loss during the flowback period. Understanding the matrix imbibition capacity and rate helps to determine the fracturing fluid volume, optimize the flowback design, and to analyze the influences on the production of shale gas.Imbibition experiments were conducted on shale samples from the Sichuan Basin, and some tight sandstone samples from the Ordos Basin. Tight volcanic samples from the Songliao Basin were also investigated for comparison. The effects of porosity, clay minerals, surfactants, and KCl solutions on the matrix imbibition capacity and rate were systematically investigated. The results show that the imbibition characteristic of tight rocks can be characterized by the imbibition curve shape, the imbibition capacity, the imbibition rate, and the diffusion rate. The driving forces of water imbibition are the capillary pressure and the clay absorption force. For the tight rocks with low clay contents,the imbibition capacity and rate are positively correlated with the porosity. For tight rocks with high clay content,the type and content of clay minerals are the most important factors affecting the imbibition capacity. The imbibed water volume normalized by the porosity increases with an increasing total clay content. Smectite and illite/smectite tend to greatly enhance the water imbibition capacity.Furthermore, clay-rich tight rocks can imbibe a volume ofwater greater than their measured pore volume. The average ratio of the imbibed water volume to the pore volume is approximately 1.1 in the Niutitang shale, 1.9 in the Lujiaping shale, 2.8 in the Longmaxi shale, and 4.0 in the Yingcheng volcanic rock, and this ratio can be regarded as a parameter that indicates the influence of clay. In addition,surfactants can change the imbibition capacity due to alteration of the capillary pressure and wettability. A 10wt% KCl solution can inhibit clay absorption to reduce the imbibition capacity. | Hong-Kui Ge Liu Yang Ying-Hao Shen Kai Ren Fan-Bao Meng Wen-Ming Ji Shan Wu | 2015 | Petroleum Science2015，12，4： | 13 |
| 5 | Vagus Nerve Stimulation for Pediatric and Adult Patients with Pharmaco-resistant Epilepsy显示文摘 | Fan-Gang Meng Fu-Min Jia Xiao-Hui Ren Yan Ge Kai-Liang Wang Yan-Shan Ma Ming Ge Kai Zhang Wen-Han HU Xin Zhang Wei Hu Jian-Guo Zhang | 2015 | Chinese Medical Journal2015，，19： | 13 |
| 6 | Characteristics of the HIV/AIDS Epidemic in Women Aged 15-49 Years from 2005 to 2012 in China显示文摘Objective To describe the characteristics of the HIV/AIDS epidemic in women aged 15-49 years in China. Methods HIV/AIDS cases from 2005 to 2012 that fulfilled the inclusion and exclusion criteria were identified on the Chinese HIV/AIDS case reporting system. Descriptive and spatial analyses were performed. Results A total of 103,559 female HIV/AIDS cases were included in our study. Based on the descriptive analysis, between 2005 and 2012, the proportion of heterosexually acquired HIV infection among women(15-49 years) increased rapidly from 35.8% to 87.4%. Approximately 60% of these cases were infected through non-marital heterosexual contact. Among older women(40-49 years), a slightly increasing trend was identified. The spatial analysis detected ‘hot spots' in the Xinjiang, Yunnan, Sichuan, Guangxi, and Chongqing provinces. The epidemic trends in these areas were predominately driven by heterosexual transmission. Conclusion Non-marital heterosexual contact is a very important factor in the HIV/AIDS epidemic in women aged 15-49 years, and the HIV infection rate in older women is increasing. Several epidemic hot spots were detected in northwestern and southwestern China. Efficient interventions are needed to control the spread of HIV/AIDS among women living in these areas. | ZHANG Xia Yan HUANG Tao FENG Yi Bing LI Meng CHEN Fang Fang LI Yin Ge JIN Shan Shan BU Kai WANG Lu | 2015 | Biomedical and Environmental Sciences2015，28，10： | 10 |
| 7 | Deep brain stimulation in the treatment of secondary dystonia显示文摘Background Dystonia is one of the most challenging movement disorders to treat.Medications and surgeries are the two methods to control dystonic symptoms.For patients with dystonia in whom symptoms are inadequately controlled with pharmacologic measures,the use of deep brain stimulation(DBS) can improve symptoms and enhance functional capacity.The best candidate for DBS is believed to be primary generalized dystonia,especially the DYT-1 type.Here,we report 9 cases of secondary dystonia to explore the feasibility,indications and complications of DBS in the treatment of secondary dystonia.Methods From July 2003 to June 2006,nine patients with secondary dystonia underwent surgery at Beijing Tiantan Hospital.Among them,2 were diagnosed as having tardive dystonia,1 had posttraumatic dystonia,3 had a history of perinatal anoxia,1 had neonatal pathologic jaundice,and 2 had no exact contributory history;MRI showed bilateral lentiform nuclei degeneration in one patient.Six patients underwent bilateral subthalamic nucleus(STN)-DBS,two underwent unilateral STN-DBS,the other underwent left STN and right globus pallidus internus(GPi)-DBS.Results With intraoperative microelectrode recording,the targeted nucleus was accurately localized.Tentative stimulation could decrease muscle tension to the same extent,but twisting was not obviously improved.Follow-up for 3 months to 3 years showed satisfactory results in 3 patients with tardive dystonia and posttraumatic dystonia and that Burke-Fahn-Marsden Dystonia Scale(BFMS) decreased by more than 90%.The improvement of symptoms was progressive along with time.The other 6 patients had slight to moderate improvement.None of them had severe surgery-related complications.One had lead fracture 16 months after surgery and the lead was then evacuated.Conclusions DBS could be an ideal treatment for patients with tardive and posttraumatic dystonia.For patients with perinatal anoxia and diffuse impairment in the basal ganglia,DBS seemed not to be a good choice.STN could be an ideal target.Intraoperative microelectrode recording and mild amelioration of muscle tension are indicators of correct target location.No severe complications occurred. | ZHANG Jian-guo,ZHANG Kai,WANG Zhong-cheng,GE Ming and MA Yu Department of Neurosurgery,Beijing Tiantan Hospital,Capital University of Medical Sciences Functional Neurosurgical Department,Beijing Neurosurgical Institute,Beijing 100050,China | 2006 | Chinese Medical Journal2006，，24： | 9 |
| 8 | Prioritizing natural-selection signals from the deep-sequencing genomic data suggests multi-variant adaptation in Tibetan highlanders显示文摘Human genetic adaptation to high altitudes(>2500 m)has been extensively studied over the last few years,but few functional adaptive genetic variants have been identified,largely owing to the lack of deep-genome sequencing data available to previous studies.Here,w e build a list of putative adaptive variants,including 63 missense,7 loss-of-function;1,298 evolutionarily conserved variants and 509 expression quantitative traits loci.Notably,the top signal of selection is located in TMEM 247,a transmembrane protein-coding gene.The Tibetan version of TMEM 247 harbors one high-frequency(76.3%)missense variant,rsl 16983452(c.248C>T;p.Ala83Val);with the T allele derived from archaic ancestry and carried by>94%of Tibetans but absent or in low frequencies(<3%)in non-Tibetan populations.The rsl 16983452-T is strongly and positively correlated with altitude and significantly associated with reduced hemoglobin concentration(p=5.78×10^-5),red blood cell count(p=5.72×10^-7)and hematocrit(p=2.57×10^-6).In particular,TMEM247-rs 116983452 shows greater effect size and better predicts the phenotypic outcome than any E P A S1 variants in association with adaptive traits in Tibetans.Modeling the interaction between TM£M247-rsl 16983452 and EPAS1 variants indicates weak but statistically significant epistatic effects.Our results support that multiple variants may jointly deliver the fitness of the Tibetans on the plateau,where a complex model is needed to elucidate the adaptive evolution mechanism. | Lian Deng Chao Zhang Kai Yuan Yang Gao Yuwen Pan Xueling Ge Yaoxi He Yuan Yuan Yan Lu Xiaoxi Zhang Hao Chen Haiyi Lou Xiaoji Wang Dongsheng Lu Jiaojiao Liu Lei Tian Qidi Feng Asifullah Khan Yajun Yang Zi-Bing Jin Jian Yang Fan Lu Jia Qu Longli Kang Bing Su Shuhua Xu | 2019 | National Science Review2019，6，6： | 9 |
| 9 | m6A demethylase FTO attenuates cardiac dysfunction by regulating glucose uptake and glycolysis in mice with pressure overload-induced heart failure显示文摘Dear Editor,N6-methyladenosine(m6A)is the most common posttranscriptional mammalian mRNA modification involved in multiple biological processes and diseases.1 However,its role in cardiac energy metabolism changes during heart failure(HF)remains elusive.Here we explored the role of m6A demethylase fat mass and obesity-associated protein(FTO)in glucose metabolism during pressure overload-induced HF.Methylated RNA immunoprecipitation sequencing(MeRIP-seq)was performed to map transcriptome-wide m6A modifications in transverse aortic constriction(TAC)and sham hearts.The number of identified m6A peaks was higher in the TAC than in the sham hearts(Fig.1a).Consistent with previous reports,the m6A peaks were enriched in 3’-untranslated regions in both the TAC and sham hearts(Fig.1b).2 The m6A peaks were characterized by the canonical RGAAR(R=A or G)motif(Fig.1c). | Beijian Zhang Hao Jiang Jian Wu Yun Cai Zhen Dong Yongchao Zhao Qinfeng Hu Kai Hu Aijun Sun Junbo Ge | 2021 | Signal Transduction and Targeted Therapy2021，6，12： | 6 |
| 10 | Chromosome-level genome of Himalayan yew provides insights into the origin and evolution of the paclitaxel biosynthetic pathway显示文摘Taxus,commonly known as yew,is a well-known gymnosperm with great ornamental and medicinal value.In this study,by assembling a chromosome-level genome of the Himalayan yew(Taxus wallichiana)with 10.9 Gb in 12 chromosomes,we revealed that tandem duplication acts as the driving force of gene family evolution in the yew genome,resulting in the main genes for paclitaxel biosynthesis,i.e.those encoding the taxadiene synthase,P450s,and transferases,being clustered on the same chromosome.The tandem duplication may also provide genetic resources for the nature to sculpt the core structure of taxoids at different positions and subsequently establish the complex pathway of paclitaxel by neofunctionalization.Furthermore,we confirmed that there are two genes in the cluster encoding isoenzymes of a known enzyme in the paclitaxel biosynthetic pathway.The reference genome of the Himalayan yew will serve as a platform for decoding the complete biosynthetic pathway of paclitaxel and understanding the chemodi-versity of taxoids in gymnosperms. | Jian Cheng Xiao Wang Xiaonan Liu Xiaoxi Zhu Zihe Li Huanyu Chu Qian Wang QianQian Lou Bijun Cai Yiqun Yang Xiaoyun Lu Kai Peng Dingyu Liu Yuwan Liu Lina Lu Huan Liu Ting Yang Qijin Ge Chengcheng Shi Guichun Liu Zhiwei Dong Xun Xu Wen Wang Huifeng Jiang Yanhe Ma | 2021 | Molecular Plant2021，14，7： | 6 |
| 11 | Synthesis and Characterization of PVA-HA-Silk Composite Hydrogel by Orthogonal Experiment显示文摘 | Dekun Zhang Kai Chen Lin Wu Dagang Wang Shirong Ge | 2012 | Journal of Bionic Engineering2012，9，2： | 6 |
| 12 | Protective effects of sirtuin 3 on titanium particle-induced osteogenic inhibition by regulating the NLRP3 inflammasome via the GSK-3β/β-catenin signalling pathway显示文摘Periprosthetic osteolysis(PPO)remains the key factor in implant failure and subsequent revision surgery and is mainly triggered by wear particles.Previous studies have shown that inhibition of osteoblastic differentiation is the most widespread incident affecting the interface of trabecular and loosening prostheses.Additionally,the NLRP3 inflammasome is activated by prosthetic particles.Sirtuin3,an NAD+-dependent deacetylase of mitochondria,regulates the function of mitochondria in diverse activities.However,whether SIRT3 can mitigate wear debris-induced osteolysis by inhibiting the NLRP3 inflammasome and enhancing osteogenesis has not been previously reported.Therefore,we investigated the role of SIRT3 during the process of titanium(Ti)particle-induced osteolysis.We revealed that upregulated SIRT3 dramatically attenuated Ti particle-induced osteogenic inhibition through suppression of the NLRP3 inflammasome and improvement of osteogenesis in vivo and in vitro.Moreover,we found that SIRT3 interference in the process of Ti particle-induced osteolysis relied on the GSK-3β/β-catenin signalling pathway.Collectively,these findings indicated that SIRT3 may serve as a rational new treatment against debris-induced PPO by deacetylase-dependent inflammasome attenuation. | Kai Zheng Jiaxiang Bai Ning Li Meng Li Houyi Sun Weicheng Zhang Gaoran Ge Xiaolong Liang Huaqiang Tao Yi Xue Yuefeng Hao Chen Zhu Yaozeng Xu Dechun Geng | 2021 | Bioactive Materials2021，6，10： | 5 |
| 13 | Optimal control of a spacecraft with deployable solar arrays using particle swarm optimization algorithm显示文摘The optimal control problem of the multibody dynamics of a spacecraft in space, modeled as a central body with one-sided connected deployable solar arrays, is investigated. The dynamical equations of motion of the spacecraft with solar arrays are derived using the multibody dynamics method. The control of the attitude motion of a spacecraft system can be transformed into the motion planning problem of nonholonomic system when the initial angular momentum is zero. These are then used to investigate the motion planning of the spacecraft during solar arrays deployment via particle swarm optimization (PSO) and results are obtained with the optimal control input and the optimal trajectory. The results of numerical simulation show that this approach is effective for the control problem of the attitude of a spacecraft during the deployment process of its solar arrays. | GE XinSheng SUN Kai | 2011 | Science China(Technological Sciences)2011，54，5： | 5 |
| 14 | Impact of chronic low to moderate alcohol consumption on blood lipid and heart energy profile in acetaldehyde dehydrogenase 2-deficient mice显示文摘 | Fan FAN Quan CAO Cong WANG Xin MA Cheng SHEN Xiang-wei LIU Li-ping BU Yun-zeng ZOU Kai HU Ai-jun SUN Jun-bo GE | 2014 | Acta Pharmacologica Sinica2014，35，8： | 5 |
| 15 | Broadband, sensitive and spectrally distinctive SnS_(2) nanosheet/PbS colloidal quantum dot hybrid photodetector显示文摘Photodetectors convert photons into current or voltage outputs and are thus widely used for spectroscopy,imaging and sensing.Traditional photodetectors generally show a consistent-polarity response to incident photons within their broadband responsive spectrum.Here we introduced a new type of photodetector employing SnS_(2) nanosheets sensitized with PbS colloidal quantum dots(CQDs)that are not only sensitive(~105AW−1)and broadband(300–1000 nm)but also spectrally distinctive,that is,show distinctive(positive or negative)photoresponse toward incident photons of different wavelengths.A careful mechanism study revealed illumination-modulated Schottky contacts between SnS_(2) nanosheets and Au electrodes,altering the photoresponse polarity toward incident photons of different wavelengths.Finally,we applied our SnS_(2) nanosheet/PbS CQDs hybrid photodetector to differentiate the color temperature of emission from a series of white light-emitting diodes(LEDs),showcasing the unique application of our novel photodetectors. | Liang Gao Chao Chen Kai Zeng Cong Ge Dun Yang Haisheng Song Jiang Tang | 2016 | Light(Science & Applications)2016，5，1： | 4 |
| 16 | Protective Efficacy of Inactivated Vaccine against SARS-CoV-2 Infection in Mice and Non-Human Primates显示文摘The ongoing coronavirus disease 2019(COVID-19)pandemic caused more than 96 million infections and over 2 million deaths worldwide so far.However,there is no approved vaccine available for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the disease causative agent.Vaccine is the most effective approach to eradicate a pathogen.The tests of safety and efficacy in animals are pivotal for developing a vaccine and before the vaccine is applied to human populations.Here we evaluated the safety,immunogenicity,and efficacy of an inactivated vaccine based on the whole viral particles in human ACE2 transgenic mouse and in non-human primates.Our data showed that the inactivated vaccine successfully induced SARS-CoV-2-specific neutralizing antibodies in mice and non-human primates,and subsequently provided partial(in low dose)or full(in high dose)protection of challenge in the tested animals.In addition,passive serum transferred from vaccine-immunized mice could also provide full protection from SARS-CoV-2 infection in mice.These results warranted positive outcomes in future clinical trials in humans. | Yan-Feng Yao Ze-Jun Wang Ren-Di Jiang Xue Hu Hua-Jun Zhang Yi-Wu Zhou Ge Gao Ying Chen Yun Peng Mei-Qin Liu Ya-Nan Zhang Juan Min Jia Lu Xiao-Xiao Gao Jing Guo Cheng Peng Xu-Rui Shen Qian Li Kai Zhao Lian Yang Xin Wan Bo Zhang Wen-Hui Wang Jia Wu Peng Zhou Xing-Lou Yang Shuo Shen Chao Shan Zhi-Ming Yuan Zheng-Li Shi | 2021 | Virologica Sinica2021，36，5： | 4 |
| 17 | Effect of electroacpuncture of Neiguan(PC 6)and Tianquan(PC 2)on skin temperature,blood perfusion,and adrenoceptor response in rats with acute myocardial ischemia显示文摘OBJECTIVE: To investigate the effect of electroacupuncture(EA) of acupoints Neiguan(PC 6) and Tianquan(PC 2) on the skin temperature, blood perfusion, and alpha1-and beta2-adrenoreceptor(α1-AR and β2-AR) protein and m RNA level in rats with acute myocardial ischemia.METHODS: Thirty male adult Wistar rats [(230 ±20) g] were randomly assigned into five groups(n = 6 each): a control group, sham operation group, model group, model group treated with EA at low frequency(L-EA, 2 Hz, 1 m A) and model group treated with EA at high frequency(H-EA, 100 Hz, 1 m A). The rat model was prepared by ligating the left anterior descending coronary artery. Electroacupuncture was performed at the left Neiguan(PC 6) for 20 min daily for 3 d. After the 3 rd time of the treatment, measurements of skin blood perfusion and temperature in Neiguan(PC 6) and Tianquan(PC 2) in all groups were made by laser speckle contrast imaging and infrared thermal image instrument. Real-time PCR and ELISA were used to measure m RNA level and protein level of α1-AR andβ2-AR in the skin tissues of Neiguan(PC 6) and Tianquan(PC 2), respectively. Serum levels of c Tn T and electrocardiogram were used to identify the state of myocardium.RESULTS: In the group receiving electroacupunture at Neiguan(PC 6), compared with control group,the skin temperature, blood perfusion, and β2-AR m RNA and protein level of model group significantly decreased(P < 0.05), α1-AR m RNA and protein level of model group significantly increased(P < 0.05);compared with model group, the skin temperature,blood perfusion, and β2-AR m RNA and protein level of L-EA significantly increased(P < 0.05), α1-AR m RNA and protein level of L-EA and H-EA significantly decreased(P < 0.05). The same trend has taken place in the former four groups of the Tianquan(PC 2).CONCLUSION: Low-or high-frequency electroacupuncture can improve the skin temperature and blood perfusion which may be induced by decreasingthelevelofα1-ARandincreasingthelevelofβ2-AR of the Neiguan(PC 6) and Tianquan(PC 2) in the rat with acute myocaidial ischemia. | Wang Dan Ji Bo Zhao Guozhen Yan Mingna Bai Hongxin Sun Xiaomin Dai Jian Lu Yawen Liu Yitian You Min Wu Jiaojuan Ge Yunpeng Su Hang Cheng Kai | 2017 | Journal of Traditional Chinese Medicine2017，37，6： | 3 |
| 18 | Projections from D2 Neurons in Different Subregions of Nucleus Accumbens Shell to Ventral Pallidum Play Distinct Roles in Reward and Aversion显示文摘The nucleus accumbens shell(NAcSh) plays an important role in reward and aversion. Traditionally, NAc dopamine receptor 2-expressing(D2) neurons are assumed to function in aversion. However, this has been challenged by recent reports which attribute positive motivational roles to D2 neurons. Using optogenetics and multiple behavioral tasks, we found that activation of D2 neurons in the dorsomedial NAcSh drives preference and increases the motivation for rewards, whereas activation of ventral NAcSh D2 neurons induces aversion. Stimulation of D2 neurons in the ventromedial NAcSh increases movement speed and stimulation of D2 neurons in the ventrolateral NAc Sh decreases movement speed. Combining retrograde tracing and in situ hybridization, we demonstrated that glutamatergic and GABAergic neurons in the ventral pallidum receive inputs differentially from the dorsomedial and ventral NAcSh. All together, these findings shed light on the controversy regarding the function of NAcSh D2 neurons, and provide new insights into understanding the heterogeneity of the NAcSh. | Yun Yao Ge Gao Kai Liu Xin Shi Mingxiu Cheng Yan Xiong Sen Song | 2021 | Neuroscience Bulletin2021，37，5： | 3 |
| 19 | Antioxidant capacity of extract from Jiangtang Xiaozhi recipe in vitro显示文摘OBJECTIVE: To evaluate the antioxidant capacity of the extract from Jiangtang Xiaozhi recipe(JXR) of in vitro.METHODS: JXR extract was prepared according to previously reported method. In vitro antioxidant assays were used in this experiment, including 1,1-Diphenyl-2-picrylhydrazyl free radical(DPPH) radical scavenging ability, 2-2’-Azinobis-(3-ethylbenzthiazoline-6-sul phonate(ABTS) radical scavenging ability, reducing power assay, fluorescence recovery after photo bleaching assay, β-carotene bleaching assay, ferric thiocyanate assay, and thiobarbituric acid method.RESULTS: DPPH, ABTS assay showed that JXR extract had distinct effect on scavenging free radicals;reducing power and ferricreducing-antioxidant power assay showed that JXR extract possessed redox ability;β-Carotene bleaching assay and antioxidant activity in a linoleic acid system using ferric thiocyanate method, thiobarbituric acid assay indicated that JXR extract could effectively inhibit lipid peroxidation, and the effect was better than that of Vitamin C.CONCLUSION: JXR extract has significant antioxidant capacity in vitro. | Song Wang Yuan Yuan Yu Naixin Gu Haike Zhou Yunfeng Chen Xigong Wang Shuaiyu Fan Kai Ge Zhengyan Jin Long Xu Yang | 2020 | Journal of Traditional Chinese Medicine2020，40，3： | 3 |
| 20 | Establishment and Optimization of the Regeneration System of Mature Embryos of Maize (Zea mays L.)显示文摘A reliable system was developed for regeneration from mature embryos derived from callus of four maize inbred lines (Liao 7980,Dan 9818,Dan 340,and Dan 5026). The protocol was mainly based on a series of experiments involving the composition of culture medium. We found that 9 μM 2,4-dichlorophenoxyacetic acid in MS medium was optimum for the induction of callus. The induction frequency of primary calli was over 85% for four inbred lines tested. The addition of L-proline (12 mM) in subculture medium significantly promoted the formation of embryogenic callus but it did not significantly enhance growth rate of callus. Efficient shoot regeneration was obtained on regeneration medium containing 2.22 μM 6-benzylaminopurine in combinations with 4.64 μM Kinetin. Regenerated shoots were rooted on half-strength MS medium containing 2.85 μM indole-3-butyric acid. This plant regeneration system provides a foundation for genetic transformation of maize. | ZHAO Cheng-hao ZHANG Li-jun GE Chao HU Kai | 2008 | Agricultural Sciences in China2008，7，9： | 3 |
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