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3篇 您的检索式:作者名="KAM Kai Man"
    题名 作者 年代 出处 被引量
1Multicenter Evaluation of the Molecular Line Probe Assay for Multidrug Resistant Mycobacterium Tuberculosis Detection in China显示文摘In order to evaluate the performance of a molecular Hain line probe assay(Hain LPA)for rapid detection of rifampicin and isoniazid resistance of Mycobacterium tuberculosis in China。LI Qiang DONG Hai Yan PANG Yu XIA Hui OU Xi Chao ZHANG Zhi Ying LI Jun Chen ZHANG Jian Kang HUAN Shi Tong CHIN Daniel P KAM Kai Man ZHAO Yan Lin 2015Biomedical and Environmental Sciences2015,28,6:13
2Utility of mycobacterial interspersed repetitive unit typing for differentiating multidrug-resistant Mycobacterium tuberculosis isolates of the Beijing family 显示文摘Kai Man Kam Chi Wai Yip Lai Wa Tse at al 2005J Clin Microbiol2005,43,1:1
3Development of An ICR Mouse Bioassay for Toxicity Evaluation in Neurotoxic Poisoning Toxins-Contaminated Shellfish显示文摘Objective To develop an ICR (female) mouse bioassay (MBA) for toxicity confirmation and evaluation of neurotoxins (brevetoxins)-contaminated shellfish. Methods Brevetoxins (BTX-B) as a causative agent of neurotoxic shellfish poisoning (NSP) under different shellfish matrices were intraperitoneally injected at different doses into mice to study their toxic effects and to differentiate the range of lethal and sublethal dosages. Their sensitivity and specificity were analyzed with 2 competitive ELISA kits for quantitative determination of standard BTX-B and dihydroBTX-B under different shellfish matrix-diluent combinations. Detection rates of MBA and two antibody-based assays for BTX-B from field NSP-positive shellfish samples were compared. Results BTX-B could be detected in shellfish tissues at concentration of 50-400 μg/100 g under shellfish matrix-Tween-saline media, which were appropriate to identify toxic shellfish at or above the regulatory limit (80 μg/100 g shellfish tissues). The LD 50 identified was 455 g/kg for BTX-B under general shellfish matrices (excluding oyster matrices) dissolved in Tween-saline. The presence of shellfish matrices, of oyster matrices in particular, retarded the occurrence of death and toxicity presentation in mice. Two antibody-based assays, even in the presence of different shellfish matrix-diluent combinations, showed acceptable results in quantifying BTX-B and dihydroBTX-B well below the regulatory limit. Conclusion The two ELISA analyses agree favorably (correlation coefficient, r 0.96; Student's t-tests, P>0.05) with the developed bioassay.WONG Chun Kwan HUNG Patricia KAM Kai Man 2013Biomedical and Environmental Sciences2013,26,5:0
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