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| 1 | Tenascin C upregulates interleukin-6 expression in human cardiac myofibroblasts via toll-like receptor 4显示文摘AIM:To investigate the effect of Tenascin C(TNC)on the expression of pro-inflammatory cytokines and matrixmetalloproteinases in human cardiac myofibroblasts(CMF).METHODS:CMF were isolated and cultured from patients undergoing coronary artery bypass grafting.Cultured cells were treated with either TNC(0.1μmol/L,24 h)or a recombinant protein corresponding to different domains of the TNC protein;fibrinogen-like globe(FBG)and fibronectin typeⅢ-like repeats(TNⅢ5-7)(both 1μmol/L,24 h).The expression of the proinflammatory cytokines;interleukin(IL)-6,IL-1β,TNFαand the matrix metalloproteinases;MMPs(MMP1,2,3,9,10,MT1-MMP)was assessed using real time RT-PCR and western blot analysis.RESULTS:TNC increased both IL-6 and MMP3(P<0.01)mR NA levels in cultured human CMF but had no significant effect on the other markers studied.The increase in IL-6 mR NA expression was mirrored by an increase in protein secretion as assessed by enzymelinked immunosorbant assay(P<0.01).Treating CMF with the recombinant protein FBG increased IL-6mR NA and protein(P<0.01)whereas the recombinant protein TNⅢ5-7 had no effect.Neither FBG nor TNⅢ5-7 had any significant effect on MMP3 expression.The expression of toll-like receptor 4(TLR4)in human CMF was confirmed by real time RT-PCR,western blot and immunohistochemistry.Pre-incubation of cells with TLR4neutralising antisera attenuated the effect of both TNC and FBG on IL-6 mR NA and protein expression.CONCLUSION:TNC up-regulates IL-6 expression in human CMF,an effect mediated through the FBG domain of TNC and via the TLR4 receptor. | Azhar Maqbool Emma J Spary Iain W Manfield Michaela Ruhmann Lorena Zuliani-Alvarez Filomena O Gamboa-Esteves Karen E Porter Mark J Drinkhill Kim S Midwood Neil A Turner | 2016 | World Journal of Cardiology2016，8，5： | 4 |
| 2 | Antioxidant capacity of phenolic phytochemicals from various cultivars of plums显示文摘 | KIM D O JEONG S W LEE C Y | 2003 | Food Chem2003，81，： | 1 |
| 3 | A comparative study on retrogradation of dee starch gels by DSC, x-ray and a-amylase methods 显示文摘 | KIM J O KIM W S SHIN M S | 1997 | Starch/Starke1997，49，2： | 1 |
| 4 | Current Sensor Application of Asymmetric Giant Magnetoimpedance in Amorphous Materials显示文摘 | Rheem Y W Kim C G Kim C O | 2003 | Sens Actuators A2003，106，： | 1 |
| 5 | Tracing Knowledge Flows using Science and Technology Indicators显示文摘 | Noh K Kim W Kwon O etal | 2007 | Informa- tion-Yamaguchi2007，10，3： | 1 |
| 6 | Advanced bone formation in mice with a dominant-negative mutation in the thyroid hormone receptorβgene due to activation of Wnt/β-catenin protein signaling显示文摘 | O′SHEA P J KIM D W LOGAN J G | 2012 | J Biol Chem2012，287，17： | 1 |
| 7 | Anti-cancer effect and structural characterization of endo-polysaccharide from cultivated mycelia oflnonotus obliquus显示文摘 | KIM Y O PARK H W KIM J H | 2006 | Life Science2006，79，1： | 1 |
| 8 | Pulmonary tuberculosis in children:evaluation with CT显示文摘 | Kim W S Moon W K Kim I O | 1997 | AJR1997，168，4： | 1 |
| 9 | Pattern of expression of cell cycle-related proteins in malignant transformation of sinonasal inverted papilloma 显示文摘 | Kim S G Lee O Y Choi J W | 2011 | Am J Rhinol Allergy2011，25，2： | 1 |
| 10 | Effect of dexamethasone in combination with caudal analgesia on postoperative pain control in day-case paediatric orchiopexy显示文摘 | Hong J Y Han S W Kim W O | 2010 | Br J Anaesth2010，105，4： | 1 |
| 11 | Stability characteristics of Pt1Ni1/C as cathode catalysts in membrane electrode assembly of polymer electrolyte membrane fuel cell显示文摘 | CHO Y H JEON T Y YOO S J LEE K S AHN M KIM O H CHO Y H LIM J W JUNG N YOON W S CHOE H SUNG Y E | 2012 | Electrochim Acta2012，59，： | 1 |
| 12 | Immuno-stimulating effect of the endo-polysaccharide produced by submerged culture of Inonotus obliquus 显示文摘 | Kim Y O Han S B Lee H W | 2005 | Life Sci2005，77，： | 1 |
| 13 | Dynamic analysis and design of a high voltage circuit breaker with spring operating mechanism显示文摘 | YOO W S KIM S O SOHN J H | 2007 | Journal of Mechanical Science and Technology2007，21，12： | 1 |
| 14 | Antioxidant capacity of phenolic phytochemicals from various cuhivars of plums显示文摘 | KIM D O JEONG S W LEE C Y | 2003 | Food Chemistry2003，81，： | 1 |
| 15 | Manufacture and industrial application of fe-based metallic glasses显示文摘 | Kim S W Namkung J Kwon O | 2012 | Materials Science Forum2012，，： | 1 |
| 16 | Excess market value, the multina- tional corporation, and Tobin's q-ratio显示文摘 | Kim W S Lyn E O | 1986 | Journal of In- ternational Business Studies1986，17，1： | 1 |
| 17 | Inactivation of Escherichia coli O157:H7 by cinnamic aldehyde purified from Cinnamomum cassia shoot 显示文摘 | Park H D Park S W Kim H O | 2004 | Food Microbiology2004，21，3： | 1 |
| 18 | Immuno-stimulating effect of the endo-polysaccharide produced by submerged culture of Inonotus obliquus 显示文摘 | Kim Y O Han S B Lee H W | 2005 | Life Sci2005，77，19： | 1 |
| 19 | Natural frequency and mode shape sensitivities of damped systems:Part Ⅰ,distinct natural frequencies显示文摘 | Lee I W Kim D O Jung G H | 1999 | J Sound Vibration1999，223，3： | 1 |
| 20 | Identification and Pharmacological Characterization of a Novel Human Melanin-concentrating Hormone Receptor, MCH-R2显示文摘 | Suke W Jiang B Kim O | 2001 | J Biol Chem2001，276，34： | 1 |
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