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| 1 | Clustered DNA damage induced by protons radiation in plasmid DNA显示文摘Clustered DNA damage is considered as a critical type of lesions induced by ionizing radiation, which can be converted into the fatal or strong mutagenic complex double strand breaks (DSBs) during damage processing in the cells. The new data show that high energy protons produce more potentially lethal DSBs than low LET radiation. In this study, plasmid DNA were used to in-vestigate and re-evaluate the biological effects induced by the protons with the LET of ~3.6 keV/μm at the molecular level in vitro, including single strand breaks (SSBs), DSBs, isolated and clustered base damages. The results of complex DNA damage detections indicated that protons at the given LET value induce about 1.6 fold more non-DSB clustered DNA damages than the prompt DSB. The DNA damage yields by protons were greater than that by γ-rays, specifically by 6 fold for the isolated type of DNA damage and 14 fold for the clustered damage. Furthermore, the spectrum of damages was also demonstrated to be depended on the radiation quality, with protons producing more DSBs relative to clusters than do γ-rays. | SUI Li WANG Yu WANG Xiao KONG FuQuan LIU JianCheng ZHOU PingKun | 2013 | Chinese Science Bulletin2013，58，26： | 1 |
| 2 | The DNA concentration effect on DNA radiation damage induced by 7Li ions and γ rays显示文摘To evaluate the influence of the DNA concentration in the aqueous solution on DNA radiation damage, the plasmid DNA in the presence or absence of Mannitol (scavenger of free radical OH·) was irradiated by 7Li ions and γ rays at various DNA concentrations. Gel electrophoresis analysis revealed that the DNA damage of single and double strand breaks induced by irradiation became more severe at lower DNA concentration. In the condition of γ-ray irradiation, most of double strand breaks (DSB) damage was neutralized and less associated with DNA concentration in the presence of mannitol. However, under 7Li irradiation, DSB damage could not be cleared by mannitol but was gradually aggravated with decreasing DNA concentrations. These findings imply that under low-LET irradiation, most of the DSB damage is generated by free radical OH? diffusion, and thus may be counteracted by scavengers, while at higher-LET irradiation, quite a fraction of DSB induction is caused by direct ionizing energy deposi- tion of heavy ions, which cannot be eliminated. This work also indicates that the proportion between free radical damage and direct ionizing damage is a constant which is independent of DNA concentra- tion when the DNA concentration is under a certain value (50 ng/μL). Our study sheds light on the un- derlying mechanisms in the DNA radiation damage process. | KONG FuQuan WANG Xiao NI MeiNan SUI Li YANG MingJian ZHAO Kui | 2008 | Chinese Science Bulletin2008，53，18： | 1 |
| 3 | Diagnosis of Proton Beam with Different Energy on 100MeV Cyclotron显示文摘The uniform state of beam and the accuracy of beam measurement are important physical parameters in radiobiological research,which will directly affect the experimental results,resulting in an increase in uncertainty of dose-effect relationship between dose and radiobiological effects.In present study,using 90 MeV protons produced by cyclotron and energy degrader,EBT3 films and TLD arrays were irradiated with protons of different energy(30,40,50,60,80and 90MeV)at the dose of 2Gy. | GONG Yihao SUI Li KONG Fuquan WANG Qiaojuan ZHU Run LIU Jiancheng | 2018 | 中国原子能科学研究院年报2018，，1： | 0 |
| 4 | Survival Rate of A375 Cell Induced by 100MeV Proton显示文摘Cell survival rate is one of the most direct and important biological indicators for evaluating the killing effectiveness of radiation on cancer cells or the radio sensitivity of cancer cells.In present study,using 100MeV(LETwater=0.8keV/μm)proton produced by cyclotron andγ-rays(LETwater=0.3keV/μm)produced by 60Co radiation source at CIAE,human malignant melanoma(A375)cells were irradiated at different doses(0,1,2,4,8Gy).The relationship between cell survival and dose was studied by detecting the cell clone formation rate,and compared with the previous results of 13.7MeV protons(LETwater=3.6keV/μm). | ZHU Run SUI Li KONG Fuquan GONG Yihao WANG Qiaojuan LIU Jiancheng | 2018 | 中国原子能科学研究院年报2018，，1： | 0 |
| 5 | Measurement of Bragg Curve of Low Energy Proton显示文摘In present study,using the methods of combination of the range shifter device and the dose film,the relationship between the depth and absorbed dose of 22 MeV of low energy proton and the curve(dot line)fitted was obtained,as shown in Fig.1,and the results compared with the theoretical calculation results(full line).The results indicated that the relative | SUI Li KONG Fuquan ZHU Run LIU Jiancheng | 2017 | Annual Report of China Institute of Atomic Energy2017，，1： | 0 |
| 6 | Diagnostics of Beam Uniformity of 100 MeV Proton Cyclotron显示文摘Beam uniformity is an important parameter in the study of radiobiological effects.In present study,using 100 MeV of proton generated by cyclotron at the dose of 1Gy,the EBT3dose film and thermo luminescent CTLD-100(LiF:Mg,Ti)detectors array were irradiated,and the radiation field uniformity was diagnosed at the exper- | ZHU Run SUI Li KONG Fuquan ZHANG Yanwen LIU Jiancheng HAN Jinhua WANG Qiaojuan | 2017 | Annual Report of China Institute of Atomic Energy2017，，1： | 0 |
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