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| 1 | Generation of Reactive Oxygen Species in Different Fractions of the Coelomocytes of Holothurian Eupentacta fraudatrix in Response to the Thermostable Toxin of Yersinia pseudotuberculosis in Vitro显示文摘Pure fraction (92%-95%) of phagocytes (FP) and a mixture of amoebocytes(62%) and morula cells (38 %) FPMC of the holothurian Eupentacta fraudatrix (Holothuroidea, Dendrochirota) were obtained by using ficoll verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin of Yersinia pseudotuberculosis (TST) at different concentrations ( 0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 h treatment, and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5 fold in FPMC compared to the control. With addition of TST, lectin stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously, ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians. | Dolmatova L. S. Eliseykina M. G. Timchenko N. F. Kovaleva A. L. Shitkova O. A | 2003 | Chinese Journal of Oceanology and Limnology2003，21，4： | 3 |
| 2 | Preparation of crystalline Mg(OH)2 nanopowder from serpentinite mineral显示文摘In this paper we describe a route to produce crystalline Mg(OH)2 nanopowders from serpentinite ore distributed in the Halilovskiy array(Russia, Orenburg region). An efficient extraction route consisting of treatment on serpentinite in 40% HNO_3 at 80 °C followed by NH_4OH titration for Mg(OH)_2 precipitation was demonstrated. In this study, crystalline Mg(OH)2 nanopowders have been synthesized by solvothermal reaction method using(Mg(NO_3)_2á6H_2O) which were obtained from serpentinite, NH4 OH as a precipitator, and hydroxyethylated nonylphenol as surface-active substance. Microstructure and phase composition of samples were investigated employing scanning electron microscopy(SEM) and transmission electron microscopy(TEM), X-ray phase analysis(XRD), and inductively coupled plasma optical emission spectroscopy(ICP-OES). XRD reveals that Mg(OH)2 nanopowder with high purity has the brucite structure. It was found that crystalline Mg(OH)_2 nanopowders exclusively consist of lamellar-like structures and the sizes of Mg(OH)_2 are 30–265 nm length or width. | V. Sirota V. Selemenev M. Kovaleva I. Pavlenko K. Mamunin V. Dokalov M. Yapryntsev | 2018 | International Journal of Mining Science and Technology2018，28，3： | 2 |
| 3 | Solution of the exit time problem for mechanical systems with fast noise显示文摘 | KOVALEVA A | 2006 | Probabilistic Engineering Mechanics2006，21，4： | 1 |
| 4 | DNA assay forrecombinant pharmaceutical substances using the real-time PCRtechnique显示文摘 | Skoblov MY Shibanova ED Kovaleva EV | 2012 | Russian J Bioorganic Chem2012，36，1： | 1 |
| 5 | Small cycteine-rich antifungal proteins from radish显示文摘 | Eggermonk K Kovaleva V | 1995 | The Plant Cell1995，7，： | 1 |
| 6 | Long-term safety and effi- cacy of oral eltrombopag for the treatment of subjects with idiopath- ic thrombocytopenie purpura(ITP) :preliminary data from the EX- TEND study 显示文摘 | Russel J B Cheng G Kovaleva L | 2007 | Blood ( ASH Annual Mecting Abstracts)2007，110，11： | 1 |
| 7 | Transmission ofinfection by flexible gastrointestinal endoscopy and bronchoscopy显示文摘 | Kovaleva J Peters FT van der Mei HC | 2013 | Clin Microbiol Rev2013，26，2： | 1 |
| 8 | Methylobacterium and its role in health care-associated infection显示文摘 | Kovaleva J Degener JE van der Mei HC | 2014 | J Clin Microbiol2014，52，5： | 1 |
| 9 | An EPR study of the photogeneration of hydroxyl radicals in aqueous solutions of titanyloctakis (pyridiniomethyl) phthalocyanine显示文摘 | Pykhtina E V Ulanova L A Kovaleva M A | 2000 | Zhurnal Fizicheskoi Khimii2000，74，12： | 1 |
| 10 | Long-term safety and efficacy of oral eltrombopag for the treatment of subjects with idiopathic thrombocytopenic purpura (ITP) : preliminary data from the EXTEND study 显示文摘 | Bussel JB Cheng G Kovaleva L | 2007 | Blood (ASH Annual Meeting Abstracts)2007，110，11： | 1 |
| 11 | Development of a monoclonal antibody-based enzyme-linked immunoabsorbent assay for the binding of gp130 to the IL-6/IL-6R complex and its competitive inhibition显示文摘 | Scheller J Kovaleva M Rabe B | 2004 | J Immunol Methods2004，291，12： | 1 |
| 12 | Formation and function of phragmoplast during successive cytokinesis stages in higher plant meiosis 显示文摘 | SHAMINA N V GORDEEVA E I KOVALEVA N M | 2007 | Cell Biol lnt2007，31，6： | 1 |
| 13 | Functional and translational analyses of a beta- glucosidase gene (glycosyl hydrolase family 1 ) isolated from the gut of the lower termite Reticulitermes flavipes 显示文摘 | Scharf ME Kovaleva ES Jadhao S | 2010 | Insect Biochem Mol Biol2010，40，8： | 1 |
| 14 | Arf6 promotes cell proliferation via the PLD-mTORC I and p38MAPK pathways显示文摘 | Knizhnik AV Kovaleva OV Komelkov AV | 2012 | J Cell Biochem2012，113，1： | 1 |
| 15 | The characteristicof the superstructural organizations of natural solid bitumens withAFM显示文摘 | Golubev Y A Kovaleva O V Philippov V N | 2003 | Microscopy and Microanalysis2003，9，03： | 1 |
| 16 | Observations andmorphological analysis of supermolecular structure,of natural bit-umens by atomic force microscopy显示文摘 | Golubev Y A Kovaleva O V Yushkin N P | 2008 | Fuel2008，87，1： | 1 |
| 17 | Cloning and subcellular location of an Arabidopsis receptor like protein that shares common features with protein sorting receptors of eukaryotic cells显示文摘 | Ahmed S U Bar-Peled M Kovaleva V | 1997 | Plant Physiol1997，114，： | 1 |
| 18 | Immunomodulato- ry effects of macrolides during community-acquired pneumonia: a literature review 显示文摘 | Kovaleva A Remmelts HH Rijkers GT | 2012 | J Antimicrob Chemother2012，67，3： | 1 |
| 19 | Adsorption of water isotopomers H20 and D20 on hypercross-linked polystyrene MN-272显示文摘 | Lanin S'N Pashkova E B Kovaleva N V | 2009 | Russian Chemical Bulletin2009，58，4： | 1 |
| 20 | Small cysteine-rich antifungal proteins from radish:their role in host defense显示文摘 | Terras F R Eggermont K Kovaleva V et ol | 1995 | Plant Cell1995，，7： | 1 |
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