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| 1 | Relationship between oxidative stress and hepatic glutathione levels in ethanol-mediated apoptosis of polarized hepatic cells显示文摘AIM:To investigate the role of reactive oxygen species(ROS) in ethanol-mediated cell death of polarized hepatic(WIF-B) cells.METHODS:In this work,WIF-B cultures were treated with pyrazole(inducer of cytochrome P4502E1,CYP2E1) and/or L-buthionine sulfoximine(BSO),a known inhibitor of hepatic glutathione(GSH),followed by evaluation of ROS production,antioxidant levels,and measures of cell injury(apoptosis and necrosis).RESULTS:The results revealed that ethanol treatment alone caused a significant two-fold increase in the activation of caspase-3 as well as a similar doubling in ROS.When the activity of the CYP2E1 was increased by pyrazole pretreatment,an additional two-fold elevation in ROS was detected.However,the CYP2E1-related ROS elevation was not accompanied with a correlative increase in apoptotic cell injury,but rather was found to be associated with an increase in necrotic cell death.Interestingly,when the thiol status of the cells was manipulated using BSO,the ethanol-induced activation of caspase-3 was abrogated.Additionally,ethanol-treated cells displayed enhanced susceptibility to Fas-mediated apoptosis that was blocked by GSH depletion as a result of diminished caspase-8 activity.CONCLUSION:Apoptotic cell death induced as a consequence of ethanol metabolism is not completely dependent upon ROS status but is dependent on sustained GSH levels. | Benita L McVicker Pamela L Tuma Kusum K Kharbanda Serene ML Lee Dean J Tuma | 2009 | World Journal of Gastroenterology2009，15，21： | 5 |
| 2 | Cellular fi bronectin stimulates hepatocytes to produce factors that promote alcohol-induced liver injury显示文摘AIM:To examine the consequences of cellular f ibronectin(cFn)accumulation during alcohol-induced injury,and inv estigate whether increased cFn could have an effect on hepatocytes(HCs)by producing factors that could cont ribute to alcohol-induced liver injury.METHODS:HCs were isolated from rats fed a control or ethanol liquid diet for four to six weeks.Exogenous c Fn(up to 7.5 μg/mL)was added to cells cultured for 20 h,and viability(lactate dehydrogenase),apoptosis(caspase activity)and se cretion of proinflammat-ory cytokines(tumor ne c rosis fac tor alpha,TNF-α and interleukin 6,IL-6),mat rix metalloproteinases(MMPs)and their inhibitors(tissue inhibitors of metall-oproteinases,TIMPs)was det ermined.Degrad ation of iodinated cFn was det ermined over a 3 h time period in the preparations.RESULTS:cFn degradation is impaired in HCs isolated from ethanol-fed animals,leading to its accumulation in the matrix.Addition of exogenous cFn did not affect viability of HCs from control or ethanolfed animals,and apoptosis was affected only at the higher concentration.Sec retion of MMPs,TIMPs,TNF-α and IL-6,however,was increased by exogenously added cFn,with HCs from ethanolfed animals showing increased susceptibility compared to the controls.CONCLUSION:These results suggest that the elevated amounts of cFn observed in alcoholic liver injury can stimulate hepatocytes to produce factors which promote further tissue damage. | Razia S Aziz-Seible Benita L McVicker Kusum K Kharbanda Carol A Casey | 2011 | World Journal of Hepatology2011，3，2： | 2 |
| 3 | An efficient method for aromatic Friedel - Crafts alkylation, acylation, benzoylation, and sulfonylation reactions 显示文摘 | Ravi P S Rajesh M K Kusum L C | 2001 | Tetrahedron2001，57，1： | 1 |
| 4 | An eflicient method for aromatic Friedel-Crafts alkylation, acylation, benzoylation, and sulfonylation reactions 显示文摘 | Ravi P S Rajesh M K Kusum L C | 2001 | Tetrahedron2001，57，1： | 1 |
| 5 | An empirical anal- ysis of the determinants of retail margins: The role of store-brand share显示文摘 | Kusum L Ailawadi K L Harlam B | 2004 | Journal of Marketing2004，68，1： | 1 |
| 6 | Pursuing the Value-Conscious Consumer: Store Brands versus National Brand Promotions显示文摘 | Ailawadi Kusum L Scott A Neslin and Karen Gedenk | 2001 | Journal of Marketing2001，65，1： | 1 |
| 7 | A real coded genetic algorithm for solving integer and mixed integer optimization problems 显示文摘 | KUSUM D SRATAP K P KANSAL M L | 2009 | AppliedMathematics and Computation2009，212，： | 1 |
| 8 | Private-label Use and Store Loyalty显示文摘 | KUSUM L A KOEN P JAN-BENEDICT E M S | 2008 | Journal of Marketing2008，72，6： | 1 |
| 9 | An empirical analysis of the determinants of retail margins :The role of store-brand share 显示文摘 | AILAWADI KUSUM L HARLAM B | 2004 | Journal of Marketing2004，68，1： | 1 |
| 10 | Applications of Social Capital in Educational Literature: A Critical Synthesis显示文摘 | Dika Sandra L and Kusum Singh | 2002 | Review of Educational Research2002，72，1： | 1 |
| 11 | Understanding retail branding:conceptual insights and research priorities显示文摘 | Kusum L Ailawadi Kevin Lane Keller | 2004 | Journal of Retailing2004，，80： | 1 |
| 12 | Aberrant post-translational protein modifications in the pathogenesis of alcohol-induced liver injury显示文摘It is likely that the majority of proteins will undergo post-translational modification, be it enzymatic or non-enzymatic. These modified protein(s) regulate activity, localization and interaction with other cellular molecules thereby maintaining cellular hemostasis. Alcohol exposure significantly alters several of these post-translational modifications leading to impairments of many essential physiological processes. Here, we present new insights into novel modifications following ethanol exposure and their role in the initiation and progression of liver injury. This critical review condenses the proceedings of a symposium at the European Society for the Biomedical Research on Alcoholism Meeting held September 12-15, 2015, in Valencia, Spain. | Natalia A Osna Wayne G Carter Murali Ganesan Irina A Kirpich Craig J Mc Clain Dennis R Petersen Colin T Shearn Maria L Tomasi Kusum K Kharbanda | 2016 | World Journal of Gastroenterology2016，22，27： | 1 |
| 13 | Understanding retail branding:Conceptual insights and research priorities显示文摘 | Ailawadi Kusum L Keller Kevin Lane | | 0，，： | 1 |
| 14 | An interactive method using genetic algorithm for multi-objective optimization problems modeled in fuzzy environment 显示文摘 | Kusum Deep Krishna Pratap Singh Kansal M L | 2011 | Expert System with Applications2011，38，： | 1 |
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