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| 1 | Antineoplastic effects of deoxyelephantopin,a sesquiterpene lactone from Elephantopusscaber, on lung adenocarcinoma (A549) cells显示文摘OBJECTIVE: Deoxyelephantopin, a sesquiterpene lactone from Elephantopus scaber, showed inhibition of the growth of various tumor cells in vitro. In the present study, we investigated the cytotoxicity and apoptosis-inducing capacity of deoxyelephantopin on lung adenocarcinoma (A549) cells. METHODS: The cytotoxic effect of deoxyelephantopin on A549 cells and normal lymphocytes was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and 50% inhibitory concentration (IC50) value was determined. The self-renewal and proliferating potential of A549 cells after treatment with deoxyelephantopin were examined by colony formation assay. Cellular morphology of deoxyelephantopin-treated cells was observed using phasecontrast microscopy. The induction of apoptosis was evaluated using acridine orange and ethidium bromide staining, Hoechst 33342 staining, terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end-labeling (TUNEL) assay, DNA fragmentation analysis and Annexin V-fluorescein isothiocyanate staining by flow cytometry. Activation of caspases was detected using fluorogenic substrate specific to caspases 2, 3, 8 and 9 and flow cytometric analysis. The total cellular DNA content and expression of cleaved poly (ADP-ribose) polymerase was also analyzed. RESULTS: Deoxyelephantopin exhibited cytotoxicity to A549 cells (IC50=12.287 μg/mL), however, there was no toxicity towards normal human lymphocytes. Deoxyelephantopin suppressed the colony-forming ability of A549 cells in a dose-dependent manner. Acridine orange, ethidium bromide and Hoechst 33342 staining showed cell shrinkage, chromosomal condensation and nuclear fragmentation, indicating induction of apoptosis. Deoxyelephantopin increased apoptosis of A549 cells, as evidenced by more TUNEL-positive cells. DNA fragmentation and Annexin V staining revealed late-stage apoptotic cell population. Deoxyelephantopin inhibited A549 cell growth by cell cycle arrest at G2/M phase and induced apoptosis through both extrinsic and intrinsic pathways. CONCLUSION: These results suggest that deoxyelephantopin has great potential as a new chemotherapeutic agent to be developed further for the treatment of lung cancer. | Farha A. Kabeer Geetha B. Sreedevi Mangalam S. Nair Dhanya S. Rajalekshmi LathaP. Gopalakrishnan Sujathan Kunjuraman Remani Prathapan | 2013 | Journal of Integrative Medicine2013，11，4： | 4 |
| 2 | Role of QuantiFERON-TB gold,interferon gamma inducible protein-10and tuberculin skin test in active tuberculosis diagnosis显示文摘 | Syed Ahamed Kabeer B Raman B Thomas A | 2010 | PLoS One2010，5，2： | 1 |
| 3 | Social Exclusion, Poverty and Discrimination: Towards an Analytical Framework显示文摘 | Kabeer N | 2000 | Brighton :IDS2000，31，4： | 1 |
| 4 | The presence of methionine or threonine at position 381 in vitronectin is correlated with proteolytic cleavage at arginine 379 显示文摘 | Tollefsen DM Weigel CJ Kabeer MH | 1990 | J Biol Chem1990，265，： | 1 |
| 5 | Role of QuantiFERON-TB gold,interferon gamma inducible protein- 10 and tuberculin skin test in active tuberculosis diagnosis 显示文摘 | Syed Ahamed Kabeer B Raman B Thomas A | 2010 | PLoS One2010，5，2： | 1 |
| 6 | Interferon gamma and interferon gamma inducible protein-10 in detecting tuberculosis infection显示文摘 | Syed Ahamed Kabeer B Paramasivam P Raja A | 2012 | J Infect2012，64，6： | 1 |
| 7 | Comparison of interferon gamma and interferon gamma- inducible protein- 10 secretion in HIV- tuberculosis patients 显示文摘 | KABEER B S SIKHAMANI R RAJA A | 2010 | AIDS2010，24，2： | 1 |
| 8 | Is IP-10 an accurate marker for detecting M tuberculosis-specific response in HIV-infected persons显示文摘 | Goletti D Raja A Syed Ahamed Kabeer B | | 0，，09： | 1 |
| 9 | Social exclusion, poverty and discrimination: To- wards an analytic framework显示文摘 | Kabeer N | 2000 | IDS Bulletin2000，31，4： | 1 |
| 10 | Role of QuantiFERON-TB gold, interferon gamma inducible protein-10 and tuberculin skin test in active tuberculosis diagnosis显示文摘 | Syed Ahamed Kabeer B Raman B Thomas A | 2010 | PLoS One2010，5，2： | 1 |
| 11 | Gender and International Migration: Globalization, Development, and Governance显示文摘 | Lourdes Beneria Carmen Diana Deere and Naila Kabeer | 2012 | Feminist Economics2012，18，2： | 1 |
| 12 | Role of QuantiFERON-TB gold,interferon gamma inducible protein-10 and tuberculin skin test in active tuberculosis diagnosis显示文摘 | Syed Ahamed Kabeer B Raman B Thomas A | | 0，，02： | 1 |
| 13 | Globalization, Gender and Poverty: Bangladesh Women Workers in Export and local Market显示文摘 | Nalia Kabeer Simeen Mahmud | 2004 | Journal of International Development2004，，16： | 1 |
| 14 | Spatiotemporal switching signals for cancer stem cell activation in pediatric origins of adulthood cancer:Towards a watch-and-wait lifetime strategy for cancer treatment显示文摘Pediatric origin of cancer stem cell hypothesis holds great promise and potential in adult cancer treatment, however; the road to innovation is full of obstacles as there are plenty of questions left unanswered. First, the key question is to characterize the nature of such stem cells (concept). Second, the quantitative imaging of pediatric stem cells should be implemented(technology). Conceptually, pediatric stem cell origins of adult cancer are based on the notion that plasticity in early life developmental programming evolves local environments to cancer. Technologically, such imaging in children is lacking as all imaging is designed for adult patients. We postulate that the need for quantitative imaging to measure space-time changes of plasticity in early life developmental programming in children may trigger research and development of the imaging technology. Such quantitative imaging of pediatric origin of adulthood cancer will help develop a spatiotemporal monitoring system to determine cancer initiation and progression. Clinical validation of such speculative hypothesis-that cancer originates in a pediatric environment-will help implement a wait-andwatch strategy for cancer treatment. | Shengwen Calvin Li Mustafa H Kabeer | 2018 | World Journal of Stem Cells2018，10，2： | 1 |
| 15 | Resources, Agency, Achievements: Reflections on Measurement of Women's Empowerment显示文摘 | Naila Kabeer | 1999 | Development and Change1999，，30： | 1 |
| 16 | Gender Equality and Women's Empowerment: A Critical Analysis of the Third Millennium Development Goal l显示文摘 | Naila Kabeer | 2005 | Gen- der & Development2005，13，： | 1 |
| 17 | Pediatric robotic surgery :lessons from a clinical experience 显示文摘 | Klein MD Langenburg SE Kabeer M | 2007 | J Laparoendosc Adv Surg Tech A2007，17，2： | 1 |
| 18 | Social exclusion, Poverty and Discrimination: towards an analytical framework 显示文摘 | Kabeer N | 2000 | IDS Bulletint2000，31，4： | 1 |
| 19 | Role of QuantiFERON-TB gold, interferon gamma inducible protein-lO and tuberculin skin test in active tuberculosis diagnosis 显示文摘 | Syed Ahamed Kabeer B Raman B Thomas A et d | 2010 | PLoS One2010，5，2： | 1 |
| 20 | Globalization, Gender and Poverty:Bangladeshi Women Workers in Export andLocal Markets显示文摘 | Kabeer N S Mahmud | 2004 | Johrnal of International Develop- ment2004，16，1： | 1 |
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