4篇
您的检索式：作者名="Kaidong XIE"
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| 1 | Structure and Properties of Oxidative Dehydrogenation Catalysts Based on MoO 3 /Al 2 O 3显示文摘 | Kaidong Chen Shuibo Xie Alexis T Bell Enrique Iglesia | 2001 | Journal of Catalysis2001，，2： | 1 |
| 2 | Structure and Properties of Oxidative Dehydrogenation Catalysts Based on MoO 3 /Al 2 O 3显示文摘 | Kaidong Chen Shuibo Xie Alexis T Bell Enrique Iglesia | 2001 | Journal of Catalysis2001，，2： | 1 |
| 3 | UNREDUCED MEGAGAMETOPHYTE FORMATION VIA SECOND DIVISION RESTITUTION CONTRIBUTES TO TETRAPLOID PRODUCTION IN INTERPLOIDY CROSSES WITH/ORAH/MANDARIN(CITRUS RETICULATA)显示文摘Seedless fruits are desirable in the citrus fresh fruit market.Triploid production via diploid x tetraploid interploidy crosses is thought to be the most efficient and widely-used strategy for the breeding of seedless citrus.Although'Orah'mandarin has desirable organoleptic qualities,seeds in the fruits weaken its market competitiveness.To produce new seedless cultivars that are similar to'Orah'mandarin,we performed three 2x x 4x crosses using'Orah'mandarin as the seed parent to regenerate triploid plantlets.A total of 182 triploid and 36 tetraploid plantlets were obtained.By analyzing their genetic origins using nine novel single nucleotide polymorphism(SNP)markers,all of the triploids and tetraploids derived from these three crosses were proven to be hybrids.Also,we demonstrated that 2n megagametophyte formation in'Orah'mandarin result in tetraploid production in these three interploidy crosses.These tetraploid plantlets were genotyped using eight pericentromeric SNP markers and nine centromere distal SNP markers.Based on the genotypes of the 2n megagametophytes,the parental heterozygosity rates in 16 SNP loci and all 2n megagametophytes were less than 50%,indicating that second division restitution was the mechanism underlying 2n megagametophyte formation at both the population and individual levels.These triploid hybrids enrich the germplasm available for seedless breeding.Moreover,the tetraploid hybrids are valuable as parents for ploidy breeding for the production of seedless citrus fruits. | Qiangming XIA Wei WANG Kaidong XIE Xiaomeng WU Xiuxin DENG Jude WGROSSER Wenwu GUO | 2021 | Frontiers of Agricultural Science and Engineering2021，8，2： | 0 |
| 4 | An efficient transient gene expression system for protein subcellular localization assay and genome editing in citrus protoplasts显示文摘Protoplast has been widely used in biotechnologies to circumvent the breeding obstacles in citrus, including long juvenility, polyembryony, and male/female sterility. The protoplast-based transient gene expression system is a powerful tool for gene functional characterization and CRISPR/Cas9 genome editing in higher plants, but it has not been widely used in citrus. In this study, the polyethylene glycol(PEG)-mediated method was optimized for citrus callus protoplast transfection, with an improved transfection efficiency of 68.4%. Consequently, the efficiency of protein subcellular localization assay was increased to 65.8%, through transient expression of the target gene in protoplasts that stably express the fluorescent organelle marker protein. The gene editing frequencies in citrus callus protoplasts reached 14.2% after transient expression of CRISPR/Cas9 constructs. We demonstrated that the intronic polycistronic tRNAgRNA(inPTG) genome editing construct was functional in both the protoplast transient expression system and epicotyl stable transformation system in citrus. With this optimized protoplast transient expression system, we improved the efficiency of protein subcellular localization assay and developed the genome editing system in callus protoplasts, which provides an approach for prompt test of CRISPR vectors. | Wenhui Yang Jiaqin Ren Wanrong Liu Dan Liu Kaidong Xie Fei Zhang Pengwei Wang Wenwu Guo Xiaomeng Wu | 2023 | Horticultural Plant Journal2023，9，3： | 0 |
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