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| 1 | Endothelial progenitor cells, potential biomarkers for diagnosis and prognosis of ischemic stroke: protocol for an observational case-control study显示文摘Ischemic stroke is a devastating,life altering event which can severely reduce patient quality of life.Despite years of research there have been minimal therapeutic advances.Endothelial progenitor cells(EPCs),stem cells involved in both vasculogenesis and angiogenesis,may be a potential therapeutic target.After a stroke,EPCs migrate to the site of ischemic injury to repair cerebrovascular damage,and their numbers and functional capacity may determine patients'outcome.This study aims to determine whether the number of circulating EPCs and their functional aspects may be used as biomarkers to identify the type(cortical or lacunar)and/or severity of ischemic stroke.The study will also investigate if there are any differences in these characteristics between healthy volunteers over and under 65 years of age.100 stroke patients(50 lacunar and 50 cortical strokes)will be recruited in this prospective,observational case-controlled study.Blood samples will be taken from stroke patients at baseline(within 48 hours of stroke)and days 7,30 and90.EPCs will be counted with flow cytometry.The plasma levels of pro-and anti-angiogenic factors and inflammatory cytokines will also be determined.Outgrowth endothelial cells will be cultured to be used in tube formation,migration and proliferation functional assays.Primary outcome is disability or dependence on day 90 after stroke,assessed by the modified Rankin Scale.Secondary outcomes are changes in circulating EPC numbers and/or functional capacity between patient and healthy volunteers,between patient subgroups and between elderly and young healthy volunteers.Recruitment started in February 2017,167 participants have been recruited.Recruitment will end in November 2019.West Midlands-Coventry&Warwickshire Research Ethics Committee approved this study(REC number:16/WM/0304)on September8,2016.Protocol version:2.0.The Bayraktutan Dunhill Medical Trust EPC Study was registered in ClinicalTrials.gov(NCT02980354)on November 15,2016.This study will determine whether the number of EPCs can be used as a prognostic or diagnostic marker for ischemic strokes and is a step towards discovering if transplantation of EPCs may aid patient recovery. | Kamini Rakkar Othman Othman Nikola Sprigg Philip Bath Ulvi Bayraktutan | 2020 | Neural Regeneration Research2020，15，7： | 7 |
| 2 | Outgrowth endothelial cells form a functional cerebral barrier and restore its integrity after damage显示文摘Breakdown of blood-brain barrier,formed mainly by brain microvascular endothelial cells(BMECs),represents the major cause of mortality during early phases of ischemic strokes.Hence,discovery of novel agents that can effectively replace dead or dying endothelial cells to restore blood-brain barrier integrity is of paramount importance in stroke medicine.Although endothelial progenitor cells(EPCs)represent one such agents,their rarity in peripheral blood severely limits their adequate isolation and therapeutic use for acute ischemic stroke which necessitate their ex vivo expansion and generate early EPCs and outgrowth endothelial cells(OECs)as a result.Functional analyses of these cells,in the present study,demonstrated that only OECs endocytosed DiI-labelled acetylated low-density lipoprotein and formed tubules on matrigel,prominent endothelial cell and angiogenesis markers,respectively.Further analyses by flow cytometry demonstrated that OECs expressed specific markers for sternness(CD34),immaturity(CD133)and endothelial cells(CD31)but not for hematopoietic cells(CD45).Like BMECs,OECs established an equally tight in vitro model of human BBB with astrocytes and pericytes,suggesting their capacity to form tight junctions.Ischemic injury mimicked by concurrent deprivation of oxygen and glucose(4 hours)or deprivation of oxygen and glucose followed by reperfusion(20 hours)affected both barrier integrity and function in a similar fashion as evidenced by decreases in transendothelial electrical resistance and increases in paracellular flux,respectively.Wound scratch assays comparing the vasculoreparative capacity of cells revealed that,compared to BMECs,OECs possessed a greater proliferative and directional migratory capacity.In a triple culture model of BBB established with astrocytes,pericytes and BMEC,exogenous addition of OECs effectively repaired the damage induced on endothelial layer in serum-free conditions.Taken together,these data demonstrate that OECs may effectively home to the site of vascular injury and repair the damage to maintain(neuro)vascular homeostasis during or after a cerebral ischemic injury. | Rais Reskiawan Abdulkadir Mansour Alwjwaj Othman Ahmad Othman Kamini Rakkar Ulvi Bayraktutan | 2020 | Neural Regeneration Research2020，15，6： | 5 |
| 3 | Successful management of hepatic artery pseudoaneurysm complicating chronic pancreatitis by stenting显示文摘A 41-year old alchoholic male with a history of chronic pancreatitis was admitted for nausea, vomiting and weight loss. Angiogram was performed and demonstrated an aneurysmal sac with a narrow neck originating from the inferior aspect of the dista portion of the proper hepatic artery. The origin of the pseudoaneurysm was covered with a 5 mm × 2.5 cm Viabahn cover stent (Gore). A repeat angiogram showed some leak and a second stent (6 mm × 2.3 cm) was deployed and overlapped with the first stent by 3 mm. Contrast was injected and a repeat angiogram demonstrated complete exclusion of the aneurysm. A repeat computerized axial tomography (CAT) scan of the abdomen after 24 h showed successful stenting. The patient had an uneventful post-operative course. | Cynthia Sudar Singh Kamini Giri Renuka Gupta Mohammed Aladdin Harinder Sawhney | 2006 | World Journal of Gastroenterology2006，12，35： | 4 |
| 4 | Characteristics of Clostridium difficile infection in patients hospitalized with myelodysplastic syndrome or acute myelogenous leukemia显示文摘AIM To evaluate factors associated with Clostridium difficile infection (CDI) and outcomes of CDI in the myelodysplastic syndrome(MDS) and acute myeloid leukemia (AML) population.METHODS After IRB approval,all MDS/AML patients hospitalized at the University of Maryland Greenebaum Comprehensive Cancer Center between August 2011 and December 2013 were identified.Medical charts were reviewed for demographics,clinical information,development of CDI,complications of CDI,and mortality.Patients with CDI,defined as having a positive stool PCR done for clinical suspicion of CDI,were compared to those without CDI in order to identify predictors of disease.A t-test was used for comparison of continuous variables and chisquare or Fisher's exact tests were used for categorical variables,as appropriate.RESULTS Two hundred and twenty-three patients (60.1% male,mean age 61.3 years,13% MDS,87% AML) had 594 unique hospitalizations during the study period.Thirtyfour patients (15.2%) were diagnosed with CDI.Factors significantly associated with CDI included lower albumin at time of hospitalization (P < 0.0001),prior diagnosis of CDI (P < 0.0001),receipt of cytarabine-based chemotherapy (P = 0.015),total days of neutropenia (P = 0.014),and total days of hospitalization (P = 0.005).Gender (P = 0.10),age (P = 0.77),proton-pump inhibitor use (P = 0.73),receipt of antibiotics (P = 0.66),and receipt of DNA hypomethylating agent-based chemotherapy (P = 0.92) were not significantly associated with CDI.CONCLUSION CDI is common in the MDS/AML population.Factors significantly associated with CDI in this population include low albumin,prior CDI,use of cytarabine-based chemotherapy,and prolonged neutropenia.In this study,we have identified a subset of patients in which prophylaxis studies could be targeted. | Kamini Shah Bryan F Curtin Christopher Chu Daniel Hwang Mark H Flasar Erik von Rosenvinge | 2017 | World Journal of Clinical Oncology2017，8，5： | 3 |
| 5 | Identification of commonly regulated protein targets and molecular pathways in PC-3 and DU145 androgen-independent human prostate cancer cells treated with the curcumin analogue 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one显示文摘Objective: To identify mutually regulated proteins in PC-3 and DU145 androgen-independent prostate cancer cell lines treated with 1,5-bis(2-hydroxyphenyl)-1,4-pentadiene-3-one(MS17), and to study the molecular pathways that contributed to the anticancer activity of MS17.Methods: PC-3 and DU145 cells were treated with 3 × EC_(50)(15 μM) concentration of MS17 for 24 h and were subjected to protein expression profiling using two-dimensional gel electrophoresis and protein identification by mass spectrometry.Selected differentially expressed proteins with significant P-value of P<0.05 and fold change over 1.5-folds were filtered through and ontologically classified.Mutually regulated proteins were ranked by fold change and identified as common protein targets of MS17.Results: Profiling data revealed that, the mutually down-regulated proteins included ACTB and ACTG associated with structural molecule activity, ACTN1 with cell cycle, ACTN4 with cell migration, HNRPK with apoptosis, PLST with morphogenesis and TERA with proteolysis.However, the expressions of CH60 and HS71 A respectively associated with response to unfolded protein demonstrated opposing regulation in PC-3 and DU145 cells.Pathway analysis of the differentially expressed proteins in PC-3 cells demonstrated the modulation of top pathways associated with cell-cell adhesion and cytoskeletal organization while in DU145 cells the pathways were associated with proteosomal degradation, regulation of electrolytes and water, regulation control of germ cells and organization of filament assembly/disassembly.Conclusions: The findings of the present study provide an understanding on the anti-tumorigenic activity of MS17 at the proteome level and warrant further research for its potential application for the management and treatment of androgen-independent prostate cancer. | Kamini Citalingam Faridah Abas Nordin H.Lajis Iekhsan Othman Rakesh Naidu | 2018 | Asian Pacific Journal of Tropical Biomedicine2018，8，9： | 2 |
| 6 | Spectral matching techniques (SMTs) and automated cropland classification algorithms (ACCAs) for mapping croplands of Australia using MODIS 250-m time-series (2000–2015) data显示文摘Mapping croplands,including fallow areas,are an important measure to determine the quantity of food that is produced,where they are produced,and when they are produced(e.g.seasonality).Furthermore,croplands are known as water guzzlers by consuming anywhere between 70%and 90%of all human water use globally.Given these facts and the increase in global population to nearly 10 billion by the year 2050,the need for routine,rapid,and automated cropland mapping year-after-year and/or season-after-season is of great importance.The overarching goal of this study was to generate standard and routine cropland products,year-after-year,over very large areas through the use of two novel methods:(a)quantitative spectral matching techniques(QSMTs)applied at continental level and(b)rule-based Automated Cropland Classification Algorithm(ACCA)with the ability to hind-cast,now-cast,and future-cast.Australia was chosen for the study given its extensive croplands,rich history of agriculture,and yet nonexistent routine yearly generated cropland products using multi-temporal remote sensing.This research produced three distinct cropland products using Moderate Resolution Imaging Spectroradiometer(MODIS)250-m normalized difference vegetation index 16-day composite time-series data for 16 years:2000 through 2015.The products consisted of:(1)cropland extent/areas versus cropland fallow areas,(2)irrigated versus rainfed croplands,and(3)cropping intensities:single,double,and continuous cropping.An accurate reference cropland product(RCP)for the year 2014(RCP2014)produced using QSMT was used as a knowledge base to train and develop the ACCA algorithm that was then applied to the MODIS time-series data for the years 2000–2015.A comparison between the ACCA-derived cropland products(ACPs)for the year 2014(ACP2014)versus RCP2014 provided an overall agreement of 89.4%(kappa=0.814)with six classes:(a)producer’s accuracies varying between 72%and 90%and(b)user’s accuracies varying between 79%and 90%.ACPs for the individual years 2000–2013 and 2015(ACP2000–ACP2013,ACP2015)showed very strong similarities with several other studies.The extent and vigor of the Australian croplands versus cropland fallows were accurately captured by the ACCA algorithm for the years 2000–2015,thus highlighting the value of the study in food security analysis. | Pardhasaradhi Teluguntla Prasad S.Thenkabail Jun Xiong Murali Krishna Gumma Russell G.Congalton Adam Oliphant Justin Poehnelt Kamini Yadav Mahesh Rao Richard Massey | 2017 | International Journal of Digital Earth2017，10，9： | 2 |
| 7 | Production,purification and characterization of an extra-celluular lipase from the yeast,Cryptococcussp S-2显示文摘 | Kamini N R Fujii T Kurosu T | 2000 | Process Biochemistry2000，36，4： | 1 |
| 8 | 显示文摘 | Kamini N R lefuji H | 2001 | Process Biochemistry2001，37，4： | 1 |
| 9 | Antimicrobial activity of garlic and onion extracts显示文摘 | KAMINI E N SYEDA A | 1983 | Pharmazie1983，38，11： | 1 |
| 10 | Lipase Catalyzed Methanolysis of Vegetable Oils in Aqueous Medium by Cryptococcus sppS-2显示文摘 | Kamini N R Iefuji H | 2001 | Process Biochemistry2001，37，4： | 1 |
| 11 | Lipase produc- tion from Aspergillus niger by solid-state fermentation using gingelly oil cake显示文摘 | Kamini N R Mala J G S Puvanakrishnan R | 1998 | Proces B iochemistry1998，33，5： | 1 |
| 12 | Microbial degradation of aliphatic and aliphatic-aromatic co-polyesters显示文摘 | Aamer Ali Shah Satoshi Kato Noboru Shintani Numbi Ramudu Kamini Toshiaki Nakajima-Kambe | 2014 | Applied Microbiology and Biotechnology2014，，8： | 1 |
| 13 | Antimicrobial activity of garlic and onion extracts 显示文摘 | KAMINI E N SYEDA A | 1983 | Pharmazie1983，38，11： | 1 |
| 14 | Lipase catalyzed methanolysis of vegetable oils in aqueous medium by cryptococcus spp S-2 显示文摘 | KAMINI N R IEFUJI H | 2001 | Process Biochemistry2001，37，4： | 1 |
| 15 | Antimicrobial activity of garlic and onion extracts显示文摘 | Kamini E Syeda A | 1983 | Pharmazie1983，38，11： | 1 |
| 16 | Lipase Catalyzed Methanolysis of Vegetable Oils in Aqueous Medium by Cryptococcus spp S-2显示文摘 | Kamini N R lefuji H | 2001 | Process Biochemistry2001，37，4： | 1 |
| 17 | Empty follicle syndrome: Successful pregnancy following dual trigger显示文摘 | K Deepika Suvarna Rathore Nupur Garg Kamini Rao | 2015 | Journal of Human Reproductive Sciences2015，，3： | 1 |
| 18 | Microbial enzyme technology as an alternative to conventional chemicals in leather industry 显示文摘 | Kamini N R Hemachander C Mala J G S | 1999 | Current Science1999，77，： | 1 |
| 19 | Antimicrobial activity of garlic and onion extracts显示文摘 | KAMINI E N SYEDA A | 1983 | Pharmazie1983，38，11： | 1 |
| 20 | Antimicrobial activity of garlic and onion extracts显示文摘 | Kamini En Syeda A | 1983 | Pharmazie1983，38，11： | 1 |
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