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| 1 | R-spondin-2 is a Wnt agonist that regulates osteoblast activity and bone mass显示文摘The R-spondin family of proteins are Wnt agonists, and the complete embryonic disruption of Rspo2 results in skeletal developmental defects that recapitulate the phenotype observed with Lrp5/6 deficiency. Previous work has shown that R-spondin-2(Rspo2, RSPO2) is both highly expressed in Wnt-stimulated pre-osteoblasts and its overexpression induces osteoblast differentiation in the same cells, supporting its putative role as a positive autocrine regulator of osteoblastogenesis. However, the role of Rspo2 in regulating osteoblastogenesis and bone formation in postnatal bone has not been explored. Here we show that limb-bud progenitor cells from Rspo2 knockout mice undergo reduced mineralization during osteoblastogenesis in vitro and have a corresponding alteration in their osteogenic gene expression profile. We also generated the first Rspo2 conditional knockout(Rspo2 floxed) mouse and disrupted Rspo2 expression in osteoblast-lineage cells by crossing to the Osteocalcin-Cre mouse line(OcnCre + Rspo2 ^(f/f)). Ocn-Cre + Rspo2 f/fmale and female mice at 1, 3, and 6 months were examined. Ocn-Cre + Rspo2 f/fmice are decreased in overall body size compared to their control littermates and have decreased bone mass. Histomorphometric analysis of1-month-old mice revealed a similar number of osteoblasts and mineralizing surface per bone surface with a simultaneous decrease in mineral apposition and bone formation rates. Consistent with this observation, serum osteocalcin in 3-month-old Ocn-Cre +Rspo2 f/fwas reduced, and bone marrow-mesenchymal stem cells from Ocn-Cre + Rspo2 f/fmice undergo less mineralization in vitro.Finally, gene expression analysis and immunohistochemistry of mature bone shows reduced beta-catenin signaling in Ocn-Cre +Rspo2 f/f. Overall, RSPO2 reduces osteoblastogenesis and mineralization, leading to reduced bone mass. | M.Noelle Knight Kannan Karuppaiah Michele Lowe Sarthak Mohanty Robert L.Zondervan Sheila Bell Jaimo Ahn Kurt D.Hankenson | 2018 | Bone Research2018，6，3： | 5 |
| 2 | Characterization and genetic diversity of sugarcane streak mosaic virus causing mosaic in sugarcane显示文摘 | R Viswanathan M Balamuralikrishnan R Karuppaiah | 2008 | Virus Genes2008，36，3： | 1 |
| 3 | InvestigationonthethermoelectricpropertiesofnanostructuredCr1-xTixSi2显示文摘 | KaruppaiahS BeaudhuinnM ViennoisR | 2013 | JournalofSolidStateChemistry2013，199，： | 1 |
| 4 | Polyubiquitination events mediate polymethylmethacrylate ( PMMA ) particle activation of NF- kappaB pathway 显示文摘 | Yamanaka Y Karuppaiah K Abu-Amer Y | 2011 | J Biol Chem2011，286，23： | 1 |
| 5 | Characterization and genetic diversity of sugarcane streak mosaic virus causing mosaic in sugarcane显示文摘 | Viswanathan R Balamuralikrishnan M Karuppaiah R | | 0，，36： | 1 |
| 6 | Duplexreverse transcription polymerase chain reaction (D-RT-PCR)-a technique for the simultaneous detection of viruses causing sugarcane mosaic显示文摘 | Viswanathan R Balamuralikrishnan M Karuppaiah R | | 0，，01： | 1 |
| 7 | Synthesis and Surface Chemistry of Nano Silver Particles显示文摘 | JANARDHANAN R KARUPPAIAH M HEBALKAR N | 2009 | Polyhedron2009，28，12： | 1 |
| 8 | In vitro evaluation of free radical scavenging activity of chitosan 显示文摘 | Kuppusamy S K Karuppaiah J | 2013 | Inter- national Journal of Pharmacy & Life Sciences2013，4，5： | 1 |
| 9 | The influence of process and patient factors on the recall of consent information in mentally competent patients undergoing surgery for neck of femur fractures显示文摘 | Khan SK Karuppaiah K Bajwa AS | 2012 | Ann R Coll Surg Engl2012，94，5： | 1 |
| 10 | Synthesis and surface chemistry of nano silver parti- cles显示文摘 | JANARDHANAN R KARUPPAIAH M HEBALKAR N | 2009 | Polyhedron2009，28，12： | 1 |
| 11 | Iden-tification of three genotypes of Sugarcane yellow leaf virus cau-sing yellow leaf disease from India and their molecular charac-terization显示文摘 | Viswanathan R Balamuralikrishnan M Karuppaiah R | | 0，，03： | 1 |
| 12 | Adsorption of chromium ion by acid activated low cost carbon- kinetic, mechanistic, thermodynamic and equilibrium studies 显示文摘 | ARIVOLI S HEMA M KARUPPAIAH M | 2008 | E-Journal of Chemistry2008，5，4： | 1 |
| 13 | Identification of three genotypes of sugarcane yellow leaf virus causing yellow leaf disease from India and their molecular characterization显示文摘 | Viswanathan R Balamuralikrishnan M Karuppaiah R | | 0，，： | 1 |
| 14 | Antioxidant and cytotoxic efficacy of chitosan on bladder cancer显示文摘 | Kuppusamy S Karuppaiah J | 2012 | Asian Pacif- ic Journal of Tropical Disease2012，2，2： | 1 |
| 15 | Polyu-biquitination events mediate polymethylmethacrylate(PMMA) particle activation of NF-kappaB pathway显示文摘 | Yamanaka Y Karuppaiah K Abu-Amer Y | 2011 | JBiol Chem2011，286，23： | 1 |
| 16 | Identification of three genotypes of sugarcane yellow leaf virus causing yellow leaf disease from India and their molecular characterization显示文摘 | R. Viswanathan M. Balamuralikrishnan R. Karuppaiah | 2008 | Virus Genes2008，，3： | 1 |
| 17 | Detection of three major RNA viruses infecting sugarcane by multiplex reverse transcription - polymerase chain reaction (multiplex-RT-PCR) 显示文摘 | Viswanathan R Karuppaiah R Balamuralikrishnan M | 2009 | Australasian Plant Pathology2009，39，1： | 1 |
| 18 | Synthesis and surface chemistry of nano silver particles显示文摘 | Revathi Janardhanan Murugan Karuppaiah Neha Hebalkar | 2009 | 28(12):2 522-2 5302009，28，12： | 1 |
| 19 | Synthe- sis and surface chemistry of nano silver particles 显示文摘 | Janardhanan R Karuppaiah M Hebalkar N | 2009 | Po- ly-hedron2009，28，12： | 1 |
| 20 | Synthesis and surface chemistry of nano silver particles 显示文摘 | Janardhanan R Karuppaiah M Hebalkar N Rao T N Timmy John | 2009 | Polyhedron2009，28，12： | 1 |
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