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| 1 | 地被菊花Fall Color体细胞胚途径再生、遗传转化及转基因植株的抗寒性检测显示文摘【目的】培育耐寒性强的地被菊花[Dendranthemagrandiflorum(Ramat.)Kitamura]新材料。【方法】以FallColor品种的幼嫩叶片为外植体,探讨胚状体诱导所需的植物生长调节剂浓度和诱导培养时间等条件。【结果】叶片外植体在添加0.75mg·L-12,4-D的诱导培养基上诱导15d,再进行分生培养,不仅能够诱导胚性愈伤组织形成,还能够诱导胚状体发生,并进一步诱导芽再生,最终93%的供试外植体通过胚状体途径获得芽的再生。通过根癌农杆菌介导法将35S启动子驱动的逆境诱导转录因子DREB1A基因导入该品种。转化株在低温下的种子发芽率、扦插苗生长以及植株露地越冬生长状况等方面都明显优于对照。【结论】本研究成功地建立了地被菊花FallColor体细胞胚再生途径,并成功地获得了具有越冬耐性的地被菊花转化株系。 | 洪波 仝征 李邱华 马超 KASUGA Mie YAMAGUCHI-SHINOZA KIKaziko 高俊平 | 2006 | 中国农业科学2006，39，7： | 28 |
| 2 | AtDREB1A基因在菊花中的异源表达提高了植株对干旱和盐渍胁迫的耐性显示文摘将携带有AtDREBlA基因,并以35S或rd29A启动子驱动的植物表达载体转入地被菊花(Dendranthema grandiflorum)的粉色品种‘Fall color’.与野生型相比,35S:DREBIA转基因植株表现出严重的生长抑制,而rd29A:DREB1A植株生长正常.RT-PCR检测表明,在胁迫条件下, AtDREB1A基因在35S:DREB1A转基因植株中呈现组成型过量表达,而在rd29A:DREB1A植株中则是受胁迫诱导型过量表达.这两种启动子驱动的转基因植株对干旱和盐溃胁迫都表现出较强的耐性,其中rd29A:DREB1A植株耐性显著强于35S:DREB1A植株.rd29A:DREB1A植株中的脯氨酸含量和SOD活性都强烈地被胁迫诱导升高,且高于35S:DREB1A植株.这些结果表明,在地被菊花中表达AtDREB1A基因可以提高植株对干旱和盐渍胁迫的耐性,同时这些耐性的升高可能与脯氨酸含量和SOD活性的上升有关. | 洪波 仝征 马男 李建科 Mie Kasuga Kazuko Yamaguchi-Shinozaki 高俊平 | 2006 | 中国科学(C辑)2006，36，3： | 25 |
| 3 | 根癌农杆菌介导的转录因子DREB1A基因在地被菊花中的遗传转化显示文摘以地被菊花(Dendranthema grandiflorum cv.White Snow)无菌苗叶片为外植体,在附加不同浓度植物生长调节剂的MS基础培养基上诱导培养,通过器官直接发生途径获得再生植株。结果表明,2.0mg/L6-BA和0.2mg/LNAA组合可获得93.8%的再生芽。将含拟南芥(Arabidopsis thaliana)逆境诱导转录因子DREB1A基因的植物表达载体pBI-DREB1A导入根癌农杆菌(Agrobacterium tumefaciens)LBA4404,农杆菌介导法遗传转化地被菊花White Snow叶盘,经PCR和PCR-Southern检测,DREB1A基因已整合到转化植株的基因组中。遗传转化过程中,预培养2d后,将OD600=0.5~0.7的根癌农杆菌稀释30倍后侵染叶盘10min,再共培养2d,有利于获得最高遗传转化效率。 | 洪波 张常青 李邱华 高俊平 KASUGA Mie SHINOZAKI-YAMAGUCHI Kazuko | 2005 | 农业生物技术学报2005，13，3： | 20 |
| 4 | Heterologous expression of the AtDREB1A gene in chrysanthemum increases drought and salt stress tolerance显示文摘DNA cassette containing an AtDREB1A cDNA and a nos terminator,driven by a cauli- flower mosaic 35S promoter,or a stress-inducible rd29A promoter,was transformed into the ground cover chrysanthemum(Dendranthema grandiflorum)'Fall Color'genome.Compared with wild type plants,severe growth retardation was observed in 35S:DREB1A plants,but not in rd29A:DREB1A plants.RT-PCR analysis revealed that,under stress conditions,the DREB1A gene was over-expressed constitutively in 35S:DREB1A plants,but was over-expressed inductively in rd29A:DREB1A plants.The transgenic plants exhibited tolerance to drought and salt stress,and the tolerance was significantly stronger in rd29A:DREB1A plants than in 35S:DREB1A plants.Proline content and SOD activity were increased inductively in rd29A:DREB1A plants than in 35S:DREB1A plants under stress conditions.These results indicate that heterologous AtDREB1A can confer drought and salt tolerance in transgenic chrysanthemum,and improvement of the stress tolerance may be related to enhancement of proline content and SOD activity. | KASUGA Mie YAMAGUCHI-SHINOZAKI Kazuko | 2006 | Science China(Life Sciences)2006，49，5： | 18 |
| 5 | Two Transcription Factors,DREB1 and DREB2,with an EREBP/AP2 DNA Binding Do main Separate Two Cellular Signal Transduction Pathways in Drought and Low-Temperature-Responsive Gene Expression, Respectively,in Arabidopsis,The Plant 显示文摘 | LIU Qiang Kasuga Mie Sakuma Yoh | 1998 | Cell1998，，10： | 1 |
| 6 | Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor显示文摘 | Mie Kasuga Qiang Liu Setsuko Miura | 1999 | Nature Biotechnology1999，17，： | 1 |
| 7 | Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought-and low-temperature-responsive gene expression, respectively, in Arahidopsis 显示文摘 | Liu Q Mie Kasuga Yoh Sakuma | 1998 | Plant Cell1998，10，： | 1 |
| 8 | Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA-binding domain separate two cellular signal transduction pathways in drought-and low-temperature-responsive gene expression respec- tively in Arabidopsis显示文摘 | Liu Qiang Kasuga Mie Saknra Yoh | 1998 | The Plant Cell1998，10，2： | 1 |
| 9 | Improving plant drought,salt,and freezing tolerance by gene transfer of a single stress-inducible transcription factor显示文摘 | Mie Kasuga Qiang Liu Setsuko Miura | 1999 | Nature Biotechnology1999，17，3： | 1 |
| 10 | Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis显示文摘 | Liu Q Mie Kasuga Yoh Sakuma | 1998 | Plant Cell1998，10，： | 1 |
| 11 | A combination of the Arabidopsis DREB1A gene and stress-inducible rd29A promoter improved drought-and low-temperature stress tolerance in Tobacco by gene transfer显示文摘 | Mie Kasuga Setsuko Miura | 2004 | Plant Cell Physiol2004，45，3： | 1 |
| 12 | Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress - inducible transcription factor显示文摘 | Mie Kasuga Qiang Liu Setsuko Miura | 1999 | Nature Bioteehnology1999，17，： | 1 |
| 13 | Improving plant drought,salt,and freezing tolerance by gene transfer of a single stress-inducible transcription factor显示文摘 | Mie Kasuga Qiang Liu Setsuko Miura | 1999 | Nature Biotechnology1999，17，： | 1 |
| 14 | Two transcription factors,DREB1 and DREB2,with and EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought-and low-temperature-responsive gene expression,respectively,in Arabidopsis显示文摘 | Liu Q Mie Kasuga Yoh Sakuma | 1998 | The Plant Cell1998，10，8： | 1 |
| 15 | Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor 显示文摘 | Mie Kasuga Qiang Liu | 1999 | Nature Biotechnolog1999，17，： | 1 |
| 16 | Improving plant dought, salt and freezing tolerance by transfer of a single stress inducible transcription factor显示文摘 | Mie Kasuga Qiang Liu | 1999 | Nature Bioteehnology1999，17，： | 1 |
| 17 | Two transcription factors, DREB1and DREB2 ,with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis显示文摘 | Mie Kasuga | 1998 | The Plant Cell1998，10，： | 1 |
| 18 | Monitoring the Expression Pattern of 1300 A rabid opsis Genes under D rought and Cold Stresses by Using a Full-Length cDNA Microarray显示文摘 | MariN arusaka HiroshiAbe Mie Kasuga Kazuko Yamaguchi-Shinozaki Piero Carninci Yoshih ide Hayashizaki and Kazuo Shinozaki | 2001 | T he P lant Cell2001，13，2： | 1 |
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