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| 1 | HBsAg loading on dendritic cells in patients with chronic hepatitis B: expressions of phenotypic molecules显示文摘BACKGROUND: The antigen reducing ability of dentritic cells (DCs), a kind of antigen presenting cells (APCs) initiating immune response, is associated with the specific immune tolerance of chronic hepatitis B(CHB) patients. However, the dysfunction of DCs can be possibly reversed by the stimulation of antigen peptides. In this study, DCs were cultured from peripheral blood monocytes (PBMCs) in patients with CHB in vitro, and the expression of phenotypic molecules on DCs loaded by different concentrations of HBsAg was observed. METHODS: Forty patients with CHB were divided randomly into 4 groups(10 patients in each group). PBMCs were isolated, and DCs were cultured after addition of granulocyte/macrophage colony-stimulating factor (GMCSF) and interleukin 4(IL-4). On the 9th day, DCs of the experimental groups were loaded at HBsAg concentrations of 2.5mg/L, 5mg/L and 10mg/L for 24 hours, whereas those of the control group were not loaded. An electron microscope was used to analyze the morphological changes of the DCs. The expression of phenotypic molecules on DCs in different groups was detected with flow cytometry. RESULTS: A combination of GM-CSF and IL-4 produced DCs from PBMCs in patients with CHB after being cultured for 9 days, whose morphological changes were tested by an electron microscope. The expression of phenotypic molecules on DCs in the control group was as low as CD83 (8.02±3.99)%, CD80(8.77±2.06)%, and MHC-DR (14.05±2.66)%. Loaded by different concentrations of HBsAg, the up-regulation of phenotypic molecules on DCs was found, with CD83(18.35±2.93)%, CD80(42.63±7.15)% and MHC-DR(47.49±6.59)% in 2.5mg/L HBsAg loading group, CD83(17.88±3.12)%, CD80(45.24± 10.93)% and MHC-DR(47.07±8.52)% in 5mg/L HBsAg loading group and CD83(16.74±2.86)%, CD80(44.59±6.99)% and MHC-DR(48.59±7.42)% in 10mg/L HBsAg loading group, respectively. Compared with the control group, the phenotypic molecules in the experimental groups were all different significantly (P<0.01), but among them, there were no differences (P>0.05). CONCLUSIONS: DCs cultured from PBMCs in the patients with CHB under the conditions of GM-CSF and IL-4 present on the typical dendritic morphology but are immature for expressing low phenotypic molecules. Loaded by different concentrations of HBsAg, the immature DCs can differentiate to mature DCs for expressing increasing phenotypic molecules. | Hua-Sheng Tong, Yi Zhang, Keng Yuan and Xin-Wen Fu Institute for Digestive Disease, Affiliated Nanfang Hospital of Nanfang Medical University, Guangzhou 510515, China Department of Infectious Disease, First Affiliated Hospital of Jiangxi Medical College Key Oncolog’y Laboratory of Institute for Medical Science, Jiangxi Province and Department of Laboratory, Jiangxi Province People’s Hospital, Nanchang 330000, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，1： | 4 |
| 2 | Transposon mouse models to elucidate the genetic mechanisms of hepatitis B viral induced hepatocellular carcinoma显示文摘The major type of human liver cancer is hepatocellular carcinoma(HCC), and there are currently many risk factors that contribute to this deadly disease. The majority of HCC occurrences are associated with chronic hepatitis viral infection, and hepatitis B viral(HBV) infection is currently a major health problem in Eastern Asia. Elucidating the genetic mechanisms associated with HBV-induced HCC has been difficult due to the heterogeneity and genetic complexity associated with this disease. A repertoire of animal models has been broadly used to study the pathophysiology and to develop potential treatment regimens for HBVassociated HCC. The use of these animal models has provided valuable genetic information and has been an important contributor to uncovering the factors involved in liver malignant transformation, invasion and metastasis. Recently, transposon-based mouse models are becoming more widely used in liver cancer research to interrogate the genome by forward genetics and also used to validate genes rapidly in a reverse genetic manner. Importantly, these transposon-based rapid reverse genetic mouse models could become crucial in testing potential therapeutic agents before proceeding to clinical trials in human. Therefore, this review will cover the use of transposon-based mouse models toaddress the problems of liver cancer, especially HBVassociated HCC occurrences in Asia. | Amy P Chiu Barbara R Tschida Lilian H Lo Branden S Moriarity Dewi K Rowlands David A Largaespada Vincent W Keng | 2015 | World Journal of Gastroenterology2015，21，42： | 2 |
| 3 | Barrier Lyapunovfunctions for the control of output-constrained nonlin-ear systems 显示文摘 | Keng P T Ge S S Francis E H | 2009 | Automatica2009，45，4： | 1 |
| 4 | Adaptive neuralcontrol for output feedback nonlinear systems using abarrier Lyapunov function 显示文摘 | Bans B R Keng P T Ge S S | 2010 | IEEE Trasactions onNeural Networks2010，21，8： | 1 |
| 5 | Determinants of consumer complaint behaviour:a study of singapore consumers显示文摘 | Keng K A Richmond D Han S | 1995 | Journal of International Consumer Marketing1995，8，2： | 1 |
| 6 | Interoganizational System and Competitive Advances 显示文摘 | Keng S | 2003 | Journal of Computer Information Systems2003，，1： | 1 |
| 7 | Single virus detection from the reactive shift of a whispering-gallery mode 显示文摘 | Vollmer F Arnold S Keng D | 2008 | Proc Natl Acad Sci USA2008，105，20： | 1 |
| 8 | Adaptive control ofelectrostatic microactuators with bidirectional drive显示文摘 | Keng P T Ge S S Francis E H | 2009 | IEEE Transactions on Control Systems Technolo-gy2009，17，2： | 1 |
| 9 | Bounded model debugging显示文摘 | Keng B Safarpour S Veneris A | 2010 | IEEE Transaction on CAD2010，29，11： | 1 |
| 10 | Toxicity of cadmium in human trophoblast cells (Jar choriocarcinoma): role of calmodulin and the calmodulin inhibitor, zaldaride maleate显示文摘 | Powlin S S Keng P C Miller R K 1997 | 1997 | Toxicol Appl Pharmacol1997，144，： | 1 |
| 11 | Seed histology of recalcitrant Eurycoma longifolia plants during germination and itsbeneficial attribute for hairy roots production 显示文摘 | Danial M Keng C L Alwee S S R S | 2005 | Journal of Medicinal Plants Research2005，5，1： | 1 |
| 12 | Anti-tumor effect of DC-CK1 gene transfection into tumor cells显示文摘 | Akiko S Jun S Keng B | 2000 | Biotherapy (Tokyo)2000，14，2： | 1 |
| 13 | Chemotaxis movement assay of Eurycoma longifolia using wild and disarmedstrains of Agrobacterium rhizogenes显示文摘 | Danial M Keng C L Alwee S S R S | 2011 | Journal of Medicinal Plants Research2011，5，8： | 1 |
| 14 | Effects of Mindfulness on Psychological Health :A Review of Empirical Studies显示文摘 | Keng S L Smoski M J Robins C J | 2011 | Clini- cal Psychology Review2011，31，6： | 1 |
| 15 | Anti-tumor effect of DC-CK1 gene transfection into tumor cells显示文摘 | Akiko S Jun S Keng B | 2000 | Biotherapy (Tokyo)2000，14，2： | 1 |
| 16 | Transformations of a class of nonlinear time-variable nluhivariable control sys-tems to block companion forms and their applications显示文摘 | Huann- keng Chiang Jason S H Tsai York-yih Sun | 1990 | Journal ofthe Franklin Institute1990，327，6： | 1 |
| 17 | Tracking and vibration control of flexible robots using shape memo- ry alloys 显示文摘 | Shuzhi S G Keng P T Ivan E V | 2006 | IEEE Transactions on Mechatronics2006，11，6： | 1 |
| 18 | Optimization of a pulsedcarbon dioxide snow jet for cleaning CMOS image sensors byusing the Taguchi method显示文摘 | SHENG C Y KENG S H YU C L | 2007 | Sensors and Actuators:A2007，139，12： | 1 |
| 19 | Assessing quality of life inSingapore: An exploratory study 显示文摘 | Keng K A Hooi W S | 1995 | Social IndicatorsResearch1995，35，1： | 1 |
| 20 | Antioxidants reduce consequences of radiation exposure 显示文摘 | OkunieffP Swarts S Keng P | 2008 | Adv Exp Med Bioi2008，614，： | 1 |
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