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| 1 | Reduced representation genome sequencing reveals patterns of genetic diversity and selection in apple显示文摘Identifying DNA sequence variations is a fundamental step towards deciphering the genetic basis of traits of interest.Here,a total of 20 cultivated and 10 wild apples were genotyped using specific-locus amplified fragment sequencing,and 39,635 single nucleotide polymorphisms with no missing genotypes and evenly distributed along the genome were selected to investigate patterns of genome-wide genetic variations between cultivated and wild apples.Overall,wild apples displayed higher levels of genetic diversity than cultivated apples.Linkage disequilibrium(LD) decays were observed quite rapidly in cultivated and wild apples,with an r^2-value below 0.2 at 440 and 280 bp,respectively.Moreover,bidirectional gene flow and different distribution patterns of LD blocks were detected between domesticated and wild apples.Most LD blocks unique to cultivated apples were located within QTL regions controlling fruit quality,thus suggesting that fruit quality had probably undergone selection during apple domestication.The genome of the earliest cultivated apple in China,Nai,was highly similar to that of Malus sieversii,and contained a small portion of genetic material from other wild apple species.This suggested that introgression could have been an important driving force during initial domestication of apple.These findings will facilitate future breeding and genetic dissection of complex traits in apple. | Baiquan Ma Liao Liao Qian Peng Ting Fang Hui Zhou Schuyler S. Korban Yuepeng Han | 2017 | Journal of Integrative Plant Biology2017，59，3： | 6 |
| 2 | Identification and isolation of Mu-flanking fragments from maize显示文摘Transposable elements have been utilized as mutagens to create mutant libraries for functional genomics. Isolation of genomic seg-ments flanking the insertion Mutator (Mu) is a key step in insertion mutagenesis studies. Herein, we adopted a modified AFLP method to identify and isolate Mu-flanking fragments from maize. The method consists of the following steps: 1) double-digestion of genomic DNA with Bgl II/Msp I and ligation of digested fragments to the Bgl II-and Msp I-adaptors; 2) enrichment of a subset of Bgl II/Msp I fragments followed by selective amplification of the Mu-flanking fragments; 3) simultaneous display of AFLP bands derived from the flanking re-gions for both insert and native Mu transposons; 4) identification and isolation of AFLP bands resulting from Mu insertions by comparing the banding profiles between Mu-induced mutants and their parental lines; and 5) confirmation of flanking fragments related to these Mu insertions. Using this approach, we have isolated flanking fragment(s) resulting from Mu insertion for every Mu-induced mutant, and one such fragment, M196-FF, is found to contain a partial sequence of the DNA topoisomerase I gene Top1. Moreover, the modified AFLP method including all restriction enzymes, adaptors and primers has been optimized in this study. The modified AFLP method has been proved to be simple and efficient in the isolation of Mu-flanking fragments and will find its usefulness in the functional genomics of maize. | Yijun Wang Guangming Yin Qin Yang Jihua Tang Xiaomin Lu Schuyler S. Korban Mmghang Xu | 2008 | Journal of Genetics and Genomics2008，35，4： | 4 |
| 3 | RNA extraction from different apple tissues rich in polyphenols and polysaccharides for cDNA library construction显示文摘 | Ksenija Gasic Alvaro Hernandez Schuyler S. Korban | 2004 | Plant Molecular Biology Reporter2004，，4： | 2 |
| 4 | Somatic embryogenesis, secondary somatic embryogenesis, and shoot organogenesis in Rosa显示文摘 | Xiangqian Li Sergei F. Krasnyanski Schuyler S. Korban | 2002 | Journal of Plant Physiology2002，，3： | 2 |
| 5 | Oral immunization of mice with transgenic tomato fruit expressing respiratory syncytial virus-F protein induces a systemic immune response显示文摘 | Jagdeep S. Sandhu Sergei F. Krasnyanski Leslie L. Domier Schuyler S. Korban Mark D. Osadjan Dennis E. Buetow | 2000 | Transgenic Research2000，，2： | 2 |
| 6 | Foods as production and delivery vehicles for human vaccines 显示文摘 | Schuyler S Korban G Sergei F | 2002 | Am Coll Nutr2002，21，： | 1 |
| 7 | Optimization of the uid A gene transfer into somatic embryos of rose via Agrobacterium tumefaciens 显示文摘 | LI X Q KRASNYANSKI S F KORBAN S S | 2002 | Plant Physiology and Biochemistry2002，40，： | 1 |
| 8 | A Bendable Finite Ele- ment for the Analysis of Flexible Cable Structures显示文摘 | Gosling P D Korban E A | 2001 | Finite Elements in Analysis and Design2001，38，1： | 1 |
| 9 | Influence of growth regulators and carbon sources on callus induction, growth and morpHogenesis from leaf tissues of peach显示文摘 | Declerck V Korban S | 1996 | Journal of Horticultural Science1996，71，1： | 1 |
| 10 | RNA extraction from different apple tissues rich in polyphenols and polysaccharides for cDNA library construction 显示文摘 | Gasic K Hemandez A Korban S S | 2004 | Plant Mol Biol Rep2004，22，： | 1 |
| 11 | Influence of media compon-ents and pH on somatic embryo induction in three genotypes of soybean显示文摘 | HOFMANN N NELSON R L KORBAN S S | 2004 | Plant Cell Tissue and Organ Culture2004，112，： | 1 |
| 12 | Effects of thidiazuron naphthaleneacetic acid, dark incubation and genotype on shoot organogenesis from Malus leaves显示文摘 | Korban S S Oconnor P A Elobeidy A | 1992 | Hort Science1992，67，3： | 1 |
| 13 | Estimating genetic diversity in New Guinea Impatiens using AFLP markers 显示文摘 | Can- J Xu M Korban SS | 2003 | Acta Hort2003，，623： | 1 |
| 14 | Development of sequence-characterized amplified regions (SCARs) from amplified fragment length polymorphism (AFLP) markers tightly linked to the Vf gene in apple显示文摘 | Xu M L Huaraeha E Korban S S | 2001 | Genome2001，44，： | 1 |
| 15 | DNA-methylation alterations and exchanges during in vitro cellular differentiation in rose (Rosa hybnda L)显示文摘 | Xu ML Li XQ Korban SS | 2004 | Theor Appl Genet2004，109，5： | 1 |
| 16 | Two critical factors are required for efficient transformation of multiple soybean cultivars: Agrobacterium strain and orientation of immature cotyledonary explant显示文摘 | Tae-Seok Ko Sangman Lee Sergei Krasnyanski Schuyler S. Korban | 2003 | Theoretical and Applied Genetics2003，，3： | 1 |
| 17 | RNA extraction from different apple tissues rich in polyphenos and polysaccharides for cDNA library construction显示文摘 | Gasic K Hernandez A Korban S S | 2004 | Plant Molecular Biology Reporter2004，22，： | 1 |
| 18 | Multiple models for Rosaeeae genomics显示文摘 | Shulaev V Korban S S Sosinski B | 2008 | Plant Physiol2008，147，： | 1 |
| 19 | AFLP-based detection of DNA methylation 显示文摘 | Xu M L Li X Q Korban S S | 2000 | Plant Mole Biol Rep2000，18，： | 1 |
| 20 | Nomenelature of the eultivated apple 显示文摘 | Korban S S Skirvin 1~ M | 1984 | Hortseienee1984，19，： | 1 |
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