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| 1 | Ron receptor-dependent gene regulation of Kupffer cells during endotoxemia显示文摘BACKGROUND: Ron receptor tyrosine kinase signaling in macrophages, including Kupffer cells and alveolar macrophages,suppresses endotoxin-induced proinflammatory cytokine/chemokine production. Further, we have also identified genes from Ron replete and Ron deplete livers that were differentially expressed during the progression of liver inflammation associated with acute liver failure in mice by microarray analyses.While important genes and signaling pathways have been identified downstream of Ron signaling during progression of inflammation by this approach, the precise role that Ron receptor plays in regulating the transcriptional landscape in macrophages, and particular in isolated Kupffer cells, has still not been investigated.METHODS: Kupffer cells were isolated from wild-type(TK+/+)and Ron tyrosine kinase deficient(TK-/-) mice. Ex vivo, the cells were treated with lipopolysaccharide(LPS) in the presence or absence of the Ron ligand, hepatocyte growth factor-like protein(HGFL). Microarray and qRT-PCR analyses were utilized to identify alterations in gene expression between genotypes.RESULTS: Microarray analyses identified genes expressed differentially in TK+/+ and TK-/- Kupffer cells basally as well as after HGFL and LPS treatment. Interestingly, our studies identified Mefv, a gene that codes for the anti-inflammatory protein pyrin, as an HGFL-stimulated Ron-dependent gene.Moreover, lipocalin 2, a proinflammatory gene, which is induced by LPS, was significantly suppressed by HGFL treatment.Microarray results were validated by qRT-PCR studies on Kupffer cells treated with LPS and HGFL.CONCLUSION: The studies herein suggest a novel mechanism whereby HGFL-induced Ron receptor activation promotes the expression of anti-inflammatory genes while inhibiting genes involved in inflammation with a net effect of diminished inflammation in macrophages. | Rishikesh M Kulkarni William D Stuart Susan E Waltz | 2014 | Hepatobiliary & Pancreatic Diseases International2014，13，3： | 6 |
| 2 | Meeting report:a hard look at the state of enamel research显示文摘The Encouraging Novel Amelogenesis Models and Ex vivo cell Lines(ENAMEL) Development workshop was held on 23 June 2017 at the Bethesda headquarters of the National Institute of Dental and Craniofacial Research(NIDCR). Discussion topics included model organisms, stem cells/cell lines, and tissues/3 D cell culture/organoids. Scientists from a number of disciplines,representing institutions from across the United States, gathered to discuss advances in our understanding of enamel, as well as future directions for the field. | ophir d klein olivier duverger wendy shaw rodrigo s lacruz derk joester janet moradian-oldak megan k pugach j timothy wright sarah e millar ashok b kulkarni john d bartlett thomas gh diekwisch pamela den besten james p simmer | 2017 | International Journal of Oral Science2017，9，4： | 3 |
| 3 | Ron receptor-dependent gene regulation in a mouse model of endotoxin-induced acute liver failure显示文摘BACKGROUND:Prior experimentation has shown that loss of the tyrosine kinase(TK) signaling domain of the Ron receptor leads to marked hepatocyte protection in a model of lipopolysaccharideinduced acute liver failure(ALF) in D-galactosamine(GalN)sensitized mice.The aim of this study was to identify the role of Ron in the regulation of hepatic gene expression.METHODS:Microarray analyses were performed on liver RNA isolated sequentially from wild-type(WT) and TK-/mice during the progression of ALF.Gene array data were validated using Western and immunohistochemistry analyses as well as with ex vivo culture systems.RESULTS:At baseline,101 genes were differentially expressed between WT and TK-/-livers,which regulate processes involved in hypoxia,proliferation,apoptosis and metabolism.One hour after ALF induction,WT livers exhibited increased cytokine expression compared to TK-/-livers,and after 4 hours,an induction of suppressor of cytokine signaling(SOCS) genes as well as JAK-STAT pathway activation were prominent in TK-/livers compared to controls.CONCLUSION:Our studies suggest a novel hepato-protective mechanism in Ron TK-/-mice wherein increased and sustained SOCS production and JAK-STAT activation in the hepatocyte may inhibit the destructive proinflammatory milieu and promote survival factors which blunt hepatic death and the ensuing development of ALF. | Rishikesh M Kulkarni Louis W Kutcher William D Stuart Daniel J Carson Mike A Leonis Susan E Waltz | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，4： | 3 |
| 4 | Preparation and Properties of Urethane/Acrylate Composite by Emulsion Polymerization Technique显示文摘 | Athawale V D Kulkarni M A | 2009 | Prog Org Coat2009，，65： | 1 |
| 5 | Deformation and Fracture Behavior of Cast and Extruded 7075Al-SiCP Composites at Room and Elevated Temperature显示文摘 | Kulkarni M D Pobi P S Prasad R C | 1996 | Mater Trans JIM1996，37，： | 1 |
| 6 | Superhydrophobic silica aerogels based on methyltrimethoxysilane precursor显示文摘 | Venkateswara R Kulkarni M M Amalnerkar D P | 2003 | J Non-crystalline Solids2003，330，： | 1 |
| 7 | Graphene oxide-polyelectrolyte nanomembranes显示文摘 | Kulkarni D D Choi I Singamaneni S S | 2010 | ASC Nano2010，4，8： | 1 |
| 8 | Biofilm producing multidrug resistant Acinetobacter baumannii: An emerging challenge显示文摘 | Badave GK Kulkarni D | 2015 | J Clin Diagn Res2015，9，1： | 1 |
| 9 | Counter propagation neural networks for fault detection and diagnosis 显示文摘 | NISHITH V TAMBE S S KULKARNI B D | 1997 | Computers & Chemical Engineering1997，21，2： | 1 |
| 10 | 查看详情显示文摘 | Humphry K J Kulkarni P M Weitz D A Morris J F Stone H A | | 0，，： | 1 |
| 11 | Ultra- robust graphene oxide-silk fibroin nanoeomposite mem- branes 显示文摘 | HU K GUPTA M K KULKARNI D D | 2013 | Advanced Materials2013，25，16： | 1 |
| 12 | An ant colony approach for clustering 显示文摘 | SHELOKAR P S JAPARAMAN V K KULKARNI B D | 2004 | Analytica Chimica Acta2004，，509： | 1 |
| 13 | Progenitor cell-trafficking is regulated by hypoxic gradients through HIF-1 induction of SDF-1显示文摘 | CERADINI D J KULKARNI A R CALLAGHAN M J | 2004 | Nat Med2004，10，8： | 1 |
| 14 | Repeat cerebrospinal fluid shunt infection in children 显示文摘 | Kulkarni AV Rabin D Lamberti-Pasculli M | 2001 | Pediatr Neurosurg2001，35，2： | 1 |
| 15 | Efficient lipase purification using reverse micellar extraction 显示文摘 | Gaikaiwari Raghavendra P Wagh Shipa A Kulkarni Bhaskar D | 2012 | Biore-source Technology2012，108，： | 1 |
| 16 | Progenitor cell trsflicking is regulated by hypoxic gradients through HIF-1 induction of SDF-1 显示文摘 | Ceradini D J Kulkarni AR Callaghan MJ | 2004 | Nat Med2004，10，8： | 1 |
| 17 | An accurate slicing procedure for layered manufacturing显示文摘 | Kulkarni P Dutta D | 1996 | CAD1996，28，9： | 1 |
| 18 | Microstructure and Properties of a Cu-Cr-Zr Alloy显示文摘 | Batra I S Dey G K Kulkarni U D | 2001 | Journal of Nuclear Materials2001，299，： | 1 |
| 19 | Extraction and purifi-cation of tannase by reverse micelle system显示文摘 | Gaikaiwari R P Wagh S A Kulkarni B D | 2012 | Separation andPurification Technology2012，，89： | 1 |
| 20 | An accurate slicing procedure for layered manufacturing 显示文摘 | Kulkarni P Dutta D | 1996 | Computer-Aided Design1996，28，9： | 1 |
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