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		题名	作者	年代	出处	被引量
	1	Artificial Esterase Based on Self-assembly Gel Microspheres Constructed from Chitosan and Amino Acids显示文摘A novel artificial esterase based on chitosan and amino acids was synthesized in the present study. The Fmoc-His and Glu were linked to chitosan by active ester method(AEM). The hydroxide radical in chitosan, imidazole group of Fmoc-His and carboxyl from Glu formed a catalytic center of natural esterase. Gel microspheres were coated with a protective layer and a supporting layer by seiassembly construction function in carboxymethylcellulose sodium(CMCS) solution. As for catalytic activity, chitosan-His-Glu was found to be more efficient than chitosan- His and chitosan-Glu in mimicking the core catalytic sites of natural esterase, and the best ratio(mass ratio) of chitosan-His-Glu:CMCS was 1:3. Furthermore, metal ions, such as Ca^2+, Mg^2+, Fe^2+, etc., were able to improve the catalytic efficiency of artificial esterase. And tlie Lineweaver-Burk plot indicated that the catalytic kinetics of artificial esterase conformed to Michaelis-Menten equation.	CAO Jing WANG Miao CHEN Weihua SHE Yongxin WANG Jing WANG Fengzhong LAO Shuibing	2019	Chemical Research in Chinese Universities2019，35，3：	0

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