5篇
您的检索式：作者名="LEI BICHENG"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Nsp2 and GP5-M of Porcine Reproductive and Respiratory Syndrome Virus Contribute to Targets for Neutralizing Antibodies显示文摘Porcine reproductive and respiratory syndrome virus(PRRSV)is characterized by its genetic variation and limited cross protection among heterologous strains.Even though several viral structural proteins have been regarded as inducers of neutralizing antibodies(NAs)against PRRSV,the mechanism underlying limited cross-neutralization among heterologous strains is still controversial.In the present study,examinations of NA cross reaction between a highly pathogenic PRRSV(HP-PRRSV)strain,JXwn06,and a low pathogenic PRRSV(LP-PRRSV)strain,HB-1/3.9,were conducted with viral neutralization assays in MARC-145 cells.None of the JXwn06-hyperimmuned pigs’sera could neutralize HB-1/3.9 in vitro and vice versa.To address the genetic variation between these two viruses that are associated with limited crossneutralization,chimeric viruses with coding regions swapped between these two strains were constructed.Viral neutralization assays indicated that variations in nonstructural protein 2(nsp2)and structural proteins together contribute to weak cross-neutralization activity between JXwn06 and HB-1/3.9.Furthermore,we substituted the nsp2-,glycoprotein2(GP2)-,GP3-,and GP4-coding regions together,or nsp2-,GP5-,and membrane(M)protein-coding regions simultaneously between these two viruses to construct chimeric viruses to test cross-neutralization reactivity with hyperimmunized sera induced by their parental viruses.The results indicated that the swapped nsp2 and GP5-M viruses increased the neutralization reactivity with the donor strain antisera in MARC-145 cells.Taken together,these results show that variations in nsp2 and GP5-M correlate with the limited neutralization reactivity between the heterologous strains HP-PRRSV JXwn06 and LP-PRRSV HB-1/3.9. | Jia Su Lei Zhou Bicheng He Xinhui Zhang Xinna Ge Jun Han Xin Guo Hanchun Yang | 2019 | Virologica Sinica2019，34，6： | 3 |
| 2 | Robust Facialexpression recognition via compressive sensing 显示文摘 | Zhang Shiqing Zhao Xiaoming Lei Bicheng | 2012 | Sensors2012，12，3： | 1 |
| 3 | Robust Facial expression recognition via compressive sensing显示文摘 | Zhang Shiqing Zhao Xiaoming Lei Bicheng | 2012 | Sensors2012，12，3： | 1 |
| 4 | On- Line Predictive Control Based on LS-SVM显示文摘 | LEI BICHENG WANG WANLIANG LI ZUXIN | 2008 | Proceed- ings of the 7th World Congress on Intelligent Control and Automation2008，，： | 1 |
| 5 | Robust facial expression reco- gnition via compressive sensing显示文摘 | Zhang Shiqing Zhao Xiaoming Lei Bicheng | 2012 | Sensors2012，12，12： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员