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| 1 | miR-200 family expression is downregulated upon neoplastic progression of Barrett's esophagus显示文摘AIM: To investigate miR-200 family expression in Barrett's epithelium, gastric and duodenal epithelia, and esophageal adenocarcinoma. METHODS: Real-time reverse transcriptase-polymerase chain reaction was used to measure miR-200, ZEB1 and ZEB2 expression. Ingenuity Pathway Analysis of miR-200 targets was used to predict biological outcomes. RESULTS: Barrett's epithelium expressed lower levels of miR-141 and miR-200c than did gastric and duodenal epithelia (P < 0.001). In silico analysis indicated roles for the miR-200 family in molecular pathways that distinguish Barrett's epithelium from gastric and duodenalepithelia, and which control apoptosis and proliferation. All miR-200 members were downregulated in adenocarcinoma (P < 0.02), and miR-200c expression was also downregulated in non-invasive epithelium adjacent to adenocarcinoma (P < 0.02). The expression of all miR-200 members was lower in Barrett's epithelium derived high-grade dysplastic cell lines than in a cell line derived from benign Barrett's epithelium. We observed signif icant inverse correlations between miR-200 family expression and ZEB1 and ZEB2 expression in Barrett's epithelium and esophageal adenocarcinoma (P < 0.05). CONCLUSION: miR-200 expression might contribute to the anti-apoptotic and proliferative phenotype of Barrett's epithelium and regulate key neoplastic processes in this epithelium. | Cameron M Smith David I Watson Mary P Leong George C Mayne Michael Z Michael Bas PL Wijnhoven Damian J Hussey | 2011 | World Journal of Gastroenterology2011，17，8： | 13 |
| 2 | Current application of proteomics in biomarker discoveryfor inflammatory bowel disease显示文摘Recently, the field of proteomics has rapidly expanded in its application towards clinical research with objectives ranging from elucidating disease pathogenesis to discovering clinical biomarkers. As proteins govern and/or reflect underlying cellular processes, the study of proteomics provides an attractive avenue for research as it allows for the rapid identification of protein profiles in a biological sample. Inflammatory bowel disease(IBD) encompasses several heterogeneous and chronic conditions of the gastrointestinal tract. Proteomic technology provides a powerful means of addressing major challenges in IBD today, especially for identifying biomarkers to improve its diagnosis and management. This review will examine the current state of IBD proteomics research and its use in biomarker research. Furthermore, we also discuss the challenges of translating proteomic research into clinically relevant tools. The potential application of this growing field is enormous and is likely to provide significant insights towards improving our future understanding and management of IBD. | Patrick PY Chan Valerie C Wasinger Rupert W Leong | 2016 | World Journal of Gastrointestinal Pathophysiology2016，7，1： | 5 |
| 3 | Evaluation of the string test for the detection of Helicobacter pylori显示文摘AIM: Helicobacter pylori can be diagnosed by invasive ornon-invasive tests but to obtain bacteria for culture andantibiotic susceptibility testing, an upper GI endoscopy isoften required. The string test may be a minimally-invasivealternative method of obtaining H. pylori samples. This studyevaluates the sensitivity and specificity of the string test inthe diagnosis of H. pylori in comparison with endoscopicmeans of diagnosis.METHODS: This was a prospective open comparative studyof patients with dyspepsia with endoscopy-based tests asgold standard (defined as a positive CLO test and antralhistology). Fasting patients swallowed the encapsulated-string(Entero-test Hp), which was withdrawn after 1 hour. Thegastric juice from the string was plated onto H. pylori-selectivemedia for culture. Helicobacter pylori was identified by typicalcolony morphology, gram stain and biochemical test results.RESULTS: Thirty dyspeptic patients were recruited of whom21 (70 %) were positive for H. pylori according to the goldstandard. The sensitivity, specificity, positive predictive value,and negative predictive value for the string test were 38 %,100 %, 100 % and 41% respectively, and for endoscopicbiopsies 81%, 100 %, 100 %, 69 % respectively (P=0.004).Logistic regression showed that only abundant growthdensity from endoscopic biopsy cultures to be a predictor ofa positive string test (P=0.018).CONCLUSION: The string test is an alternative method toendoscopy in obtaining H. pylori but has a low sensitivitycompared to endoscopic biopsies. | Rupert WL Leong Ching C Lee Thomas KW Ling WaiK Leung Joseph JY Sung | 2003 | World Journal of Gastroenterology2003，9，2： | 3 |
| 4 | Engineering functionally graded tissue engineering scaffolds显示文摘 | Leong K F Chua C K Sudarmadji N | 2008 | J Mech Behav Biomed Mater2008，1，2： | 1 |
| 5 | Factors affecting the filter paper method for total and matric suction measurements显示文摘 | LEONG E C HE L RAHARDJO H | 2002 | Geotechnieal Testing Journal2002，25，3： | 1 |
| 6 | On the reconfiguration of de- gradable VLSI/WSI arrays显示文摘 | Low C P Leong H W | 1997 | IEEE Trans Comput- er-Aided Design of Integrated Circuits and Systems1997，16，10： | 1 |
| 7 | Call Admission Control for Integrated On/Off Voice and Best-Effort Data Services in Mobile Cellular Communications显示文摘 | Leong C W Weihua Zhuang Yu Cheng | 2004 | IEEE Transactions on Communications2004，52，： | 1 |
| 8 | Response of a residual soil slope to rainfall显示文摘 | Rahardjo H Lee T T Leong E C Rezaur R B | 2005 | Canadian Geotechnical Journal2005，42，： | 1 |
| 9 | Application of flexible printed circuit board (FPCB) in personal computer motherboards: Focusing on mechanical performance 显示文摘 | LEONG W ABDULLAH M KHOR C | 2012 | Microelectronics Reliability2012，52，4： | 1 |
| 10 | Infiltration effects on stability of a residual soil slope显示文摘 | GASMO J M RAHARDJO H LEONG E C | 2000 | Computers and Geotechnics2000，26，2： | 1 |
| 11 | Engineering functionally graded tissue engineering scaffolds显示文摘 | LEONG K F CHUA C K SUDARMADJI N | 2008 | Journal of the Mechanical Behavior of Biomedical Materials2008，1，： | 1 |
| 12 | Depletion of langerhans cells in human papillomavirus type 16-infected skin is associated with E6-mediated down regulation of E-cadherin显示文摘 | MATTHEWS K LEONG C M BAXTER L | 2003 | J Virol2003，77，： | 1 |
| 13 | Infiltration Effects on Stability of a Residual Soil Slope显示文摘 | Gasmo J M Rahardjo H Leong E C | 2000 | Computers and Geote Chanics2000，26，2： | 1 |
| 14 | Infiltration effects on stability of a residual soil slope显示文摘 | CASMO J M RAHARDJO H LEONG E C | 2000 | Computers & Geotechnics2000，26，： | 1 |
| 15 | Receptor- Mediated Self - Assembly of Multi - Component Magnetic Nanowires显示文摘 | Salem A K Chao J Leong K W Searson P C | 2004 | Advanced Materials2004，16，3： | 1 |
| 16 | Elastoplastic model for unsaturated soil with incorporation of the soil-water characteristic curve 显示文摘 | THU T M RAHARDJO H LEONG E C | 2007 | Canadian Geotechnicai Journal2007，44，1： | 1 |
| 17 | Full bandwaveguide-to- microstrip probe transitions显示文摘 | LEONG Y C SANDER W | 1999 | IEEE MTT-S1999，4，： | 1 |
| 18 | Solid freeform fabrication of three-dimensional scaffolds for engineering replacement tissues and organs 显示文摘 | K F Leong C M Cheah C K Chua | 2003 | Biomaterials2003，24，： | 1 |
| 19 | A numerical study of solidification of n-hexadecane based on the enthalpy formulation 显示文摘 | Duan Q Tan F L Leong K C | 2002 | Journal of Materials Processing Technology2002，120，1： | 1 |
| 20 | Infiltration characteristics of two instrumented residual soil slopes 显示文摘 | Tsaparas I Rahardjo H Toll D G Leong E C | 2003 | Canadian Geotechnical Journal2003，40，： | 1 |
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