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| 1 | Efficient generation of transgenic chickens using the spermatogonial stem cells in vivo and ex vivo transfection显示文摘The highly efficient novel methods to produce transgenic chickens were established by directly in-jecting the recombinant plasmid containing green fluorescent protein (GFP) gene into the cock's testis termed as testis-medianted gene transfer (TMGT), and transplanting transfected spermatogonial stem cells (TTSSCs). For the TMGT approach,four dosages of pEGFP-N1 DNA/cationic polymer complex were injected intratesticularly. The results showed: (1) 48 h after the injection,the percentages of testis cells expressing GFP were 4.0%, 8.7%, 10.2% and 13.6% in the 50, 100, 150 and 200 μg/mL group, re-spectively. The difference from the four dosage groups was significant (P<0.05). On day 25 after the injection, a dosage-dependent and time-dependent increase in the number of transgenic sperm was observed. The percentages of gene expression reached the summit and became stable from day 70 to 160, being 12.7%, 12.8%, 15.9% and 19.1%, respectively. The difference from the four dosage groups was also significant (P<0.05). (2) 70 d after the injection, strong green fluorescent could be observed in the seminiferous tubules by whole-mount in-situ hybridization. (3) 70 d after the injection, the semen was collected and used to artificially inseminate wild-type females. The blastoderms of F1 and F2 transgenic chicken expressed GFP were 56.2% (254/452) and 53.2% (275/517), respectively. The detec-tion of polymerase chain reaction (PCR) of F1 and F2 transgenic chicken blood genomic DNA showed that 56.5% (3/23) of F1 and 52.9% (9/17) of F2 were positive. Southern blot showed GFP DNA was in-serted in their genomic DNAs. (4) Frozen whole mount tissue sections of F1 and F2 transgenic chicken liver, heart, kidney and muscle showed that the rates of green fluorescent positive were between 50.0% and 66.7%. (5) With the TTSSCs method, SSCs ex vivo transfected with GFP were transplanted into recipient roosters whose endogenic SSCs had been resoluted. The donor SSCs settled and GFP ex-pression became readily detectable in the frozen whole mount tissue sections of recepient testes. Moreover, sperms carrying GFP could be produced normally. The results of artificially inseminating wild-type females with these sperms showed 12.5% (8/64) of offspring embryo expressed GFP and 11.1% (2/18) hatched chicks were tested transgenic. Our data therefore suggest TMGT and TTSSCs are the feasible methods for the generation of transgenic chickens. | LI BiChun SUN GuoBo SUN HuaiChang XU Qi GAO Bo ZHOU GuanYue ZHAO WenMing WU XinSheng BAO WenBin YU Fei WANG KeHua CHEN GuoHong | 2008 | Science China(Life Sciences)2008，51，8： | 13 |
| 2 | Selection of an Effective Small Interference RNA to Silence Myostatin Gene Expression in Sheep Fibroblast Cells显示文摘 | Jian Lu Dan Sun Lingyang Xu Guobin Lu Fuping Zhao Caihong Wei Li Zhang Jiatong Ding Bichun Li Lixin Du | 2012 | Biochemical Genetics2012，，11： | 1 |
| 3 | Kinematics Analysis of Motion Simulation Subsystem for Unmanned Vehicle显示文摘Unmanned vehicle has attracted wide attention and interests throughout the world since it first deputed in the 1960s. However, the experimental methods for unmanned vehicle's intelligent behavior, such as semi-physical simulation and motion subsystem, have not been widely explored. First, the requirements of the motion subsystem in unmanned vehicle semi-physical facility are analyzed, and a six DOF parallel manipulator is selected to reproduce the pose of the vehicle. The link lengths of the motion subsystem are worked out under the given rotational angles of the vehicle. According to the geometric properties of tetrahedron, three joint positions of the top platform are determined, and the rest are obtained from the first three position vectors. Six constraint equations are set up based on the vertices on the top platform and the link lengths. In order to solve the six angle variables, a numerical algorithm built on the Newton-Raphson iterative method is presented, which is based on Taylor series expansion of six constraint equations. The pose of the top platform is ultimately calculated. The eigenvalues of the top platform are solved to obtain the natural frequencies of the motion subsystem. The coordinates of six joint centers on the top platform and six constraint equations can be realized by simple algebraic manipulation, which allows significant abbreviation in the formulation and provides a systematic way of obtaining the kinematic solution of the parallel manipulator. A numerical example is given and its efficacy is demonstrated by the inverse kinematics. The computation strategy based on tetrahedron method and Newton-Raphson iterative method provide a simple and cost-effective method for solving forward kinematics of six DOF parallel manipulators, and this method sheds light on other parallel manipulators. | TANG Guoming MEI Tao LIU Wei LI Bichun | 2011 | Chinese Journal of Mechanical Engineering2011，24，6： | 1 |
| 4 | Improvement Strategies of Meteorological Movie and Television Services Facing Public Needs显示文摘The development course of China's meteorological movie and television was analyzed firstly,and then the improvement strategies of meteorological movie and television services facing public needs were put forward based on the statistical results of a meteorological service questionnaire. | Xiaoli Wang Bichun Li | 2013 | Meteorological and Environmental Research2013，4，8： | 0 |
| 5 | The First Crested Duck Genome Reveals Clues to Genetic Compensation and Crest Cushion Formation显示文摘The Chinese crested(CC)duck is a unique indigenous waterfowl breed,which has a crest cushion that affects its survival rate.Therefore,the CC duck is an ideal model to investigate the genetic compensation response to maintain genetic stability.In the present study,we first generated a chromosome-level genome of CC ducks.Comparative genomics revealed that genes related to tissue repair,immune function,and tumors were under strong positive selection,indicating that these adaptive changes might enhance cancer resistance and immune response to maintain the genetic stability of CC ducks.We also assembled a Chinese spot-billed(Csp-b)duck genome,and detected the structural variations(SVs)in the genome assemblies of three ducks(i.e.,CC duck,Csp-b duck,and Peking duck).Functional analysis revealed that several SVs were related to the immune system of CC ducks,further strongly suggesting that genetic compensation in the anti-tumor and immune systems supports the survival of CC ducks.Moreover,we confirmed that the CC duck originated from the mallard ducks.Finally,we revealed the physiological and genetic basis of crest traits and identified a causative mutation in TAS2R40 that leads to crest formation.Overall,the findings of this study provide new insights into the role of genetic compensation in adaptive evolution. | Guobin Chang Xiaoya Yuan Qixin Guo Hao Bai Xiaofang Cao Meng Liu Zhixiu Wang Bichun Li Shasha Wang Yong Jiang Zhiquan Wang Yang Zhang Qi Xu Qianqian Song Rui Pan Lingling Qiu Tiantian Gu Xinsheng Wu Yulin Bi Zhengfeng Cao Yu Zhang Yang Chen Hong Li Jianfeng Liu Wangcheng Dai Guohong Chen | 2023 | Genomics, Proteomics & Bioinformatics2023，21，3： | 0 |
| 6 | Mesenchymal stem cells:As a multi-target cell therapy for clearingβ-amyloid deposition in Alzheimer’s disease显示文摘Extracellularβ-amyloid(Aβ)plaques and neurofibrillary tangles(NFTs)are the pathological hallmarks of Alzheimer’s disease(AD).Studies have shown that aggregates of extracellular Aβcan induce neuroinflammation mediated neurotoxic signaling through microglial activation and release of pro-inflammatory factors.Thus,modulation of Aβmight be a potential therapeutic strategy for modifying disease progression.Recently,a large number of reports have confirmed the beneficial effects of mesenchymal stem cells(MSCs)on AD.It is believed to reduce neuroinflammation,reduce Aβamyloid deposits and NFTs,increase acetylcholine levels,promote neurogenesis,reduce neuronal damage,and improve working memory and cognition.In this review,we focus on the role of MSCs in clearing Aβdeposition.MSCs have the potential to modulate Aβ-related microenvironments via enhancement of autophagy,proteolysis of Aβaggregates,phagocytic clearance of Aβby microglial M2 polarization,decrease oxidative stress(OS),and correction of abnormal sphingolipid(SL)metabolism.With advantages in clinical applications,these data suggest that the use of MSCs as a multi-target modulator of Aβwould be an effective therapeutic approach in AD. | RUXIN ZHANG CHENGGANG LI RUOCHEN DU YITONG YUAN BICHUN ZHAO YUJUAN ZHANG CHUNFANG WANG | 2022 | BIOCELL2022，46，3： | 0 |
| 7 | Gene expression of granulosa and cumulus cells: The prospect inpredicting the quality and developmental competence of oocytesin vitro maturation显示文摘In vitro maturation(IVM),a promising assisted reproductive technology(ART),has been evolving in clinical trials and applications.There is a huge potential demand for IVM in clinical practice because it reduces the stimulation of gonadotropins to patients and provides evidence for the safety of neonatal birth.Unfortunately,the maturation rate of oocytes in vitro is not as high as it is in vivo due to a different microenvironment.Moreover,there are still controversies in predicting the developmental capability of oocytes in IVM.The granulosa cells(GCs)and cumulus cells(CCs),closely surrounding the oocytes,play a critical role in oocytes development,while some studies have shown that they can reflect the quality of oocytes.Many studies have been conducted in terms of oocyte quality in transcriptional level in GCs and CCs of mice,Xenopus africanus,and Homo sapiens,which provides important enlightenment for the successful clinical application of IVM.However,no comprehensive reviews about how gene expression profiles affect oocytes quality have been reported.This review aimed to elucidate the gene expression profiles of GCs and CCs that have effects on the quality and developmental competence of oocytes maturation in vitro.And we also put forward a possible idea for ART in the future,integrating all gene expression profiles of GCs and CCs and predicting the quality of the oocytes. | BICHUN ZHAO XUEQING WU YITONG YUAN YUANTAO GAO XIAO LI RUOCHEN DU SUMING XU RUXIN ZHANG CHUNFANG WANG | 2020 | BIOCELL2020，44，4： | 0 |
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