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| 1 | Embryonic NANOG Activity Defines Colorectal Cancer Stem Cells and Modulates through AP1‐ and TCF‐dependent Mechanisms显示文摘 | Elsayed E. Ibrahim Roya Babaei‐Jadidi Anas Saadeddin Bradley Spencer‐Dene Sina Hossaini Mohammed Abuzinadah Ningning Li Wakkas Fadhil Mohammad Ilyas Dominique Bonnet Abdolrahman S. Nateri | 2012 | STEM CELLS2012，，10： | 1 |
| 2 | Generalized Riemann problem for Euler system Dedicated to Professor LI TaTsien on the Occasion of His 80th Birthday显示文摘This article is a survey on the progress in the study of the generalized Riemann problems for MD Euler system. A new result on generalized Riemann problems for Euler systems containing all three main nonlinear waves(shock, rarefaction wave and contact discontinuity) is also introduced. | CHEN ShuXing LI DeNing | 2017 | Science China Mathematics2017，60，4： | 1 |
| 3 | ATM Optical Switching Technology显示文摘2.5 Gbit/s ATM optical switching experimental system has been achieved for the first time in P.R.China.This poper describes the architecture of the ATM optical switching system.and gives some key technologies forrealization,e.g. photonic switching matrix, header identification, etc. In addition, the performance, analysis andexperimental results are given. | JI Yue-feng LI Jia-ai CHEN Peng(Institute of Telecommunication Engineering, Dening University of Posts and Telecommunications. Beijing, 100876, P. R. China) | 1999 | The Journal of China Universities of Posts and Telecommunications1999，6，2： | 0 |
| 4 | Development of novel-nanobody-based lateral-flow immunochromatographic strip test for rapid detection of recombinant human interferon a2b显示文摘Recombinant human interferon a2b(rhIFNa2b)is widely used as an antiviral therapy agent for the treatment of hepatitis B and hepatitis C.The current identification test for rhIFNa2b is complex.In this study,an anti-rhIFNa2b nanobody was discovered and used for the development of a rapid lateral flow strip for the identification of rhIFNa2b.RhIFNa2b was used to immunize an alpaca,which established a phage nanobody library.After five steps of enrichment,the nanobody I22,which specifically bound rhIFNa2b,was isolated and inserted into the prokaryotic expression vector pET28a.After subsequent purification,the physicochemical properties of the nanobody were determined.A semiquantitative detection and rapid identification assay of rhIFNa2b was developed using this novel nanobody.To develop a rapid test,the nanobody I22 was coupled with a colloidal gold to produce lateral-flow test strips.The developed rhIFNa2b detection assay had a limit of detection of 1 mg/mL.The isolation of I22 and successful construction of a lateral-flow immunochromatographic test strip demonstrated the feasibility of performing ligand-binding assays on a lateral-flow test strip using recombinant protein products.The principle of this novel assay is generally applicable for the rapid testing of other commercial products,with a great potential for routine use in detecting counterfeit recombinant protein products. | Xi Qin Maoqin Duan Dening Pei Jian Lin Lan Wang Peng Zhou Wenrong Yao Ying Guo Xiang Li Lei Tao Youxue Ding Lan Liu Yong Zhou Chuncui Jia Chunming Rao Junzhi Wang | 2022 | Journal of Pharmaceutical Analysis2022，12，2： | 0 |
| 5 | Identification of SNP markers correlated with the tolerance of low-salinity challenge in swimming crab(Portunus trituberculatus)显示文摘Water salinity condition is an important factor for artificial propagation of the swimming crab(Portunus trituberculatus). Low salinity(LS)-resistant strains are preferred by crab industries. Single nucleotide polymorphism(SNP), the third generation of molecular markers, can be utilized in the breeding of LS-resistant species of P. trituberculatus. Our earlier study identified 615 genes differentially expressed in low-salinity stress compared to the controls. Although thousands of SNP loci have been found, it is hard to identify a SNP marker in correlation with a desired trait. In this study, time-of-flight mass spectrometry(TOF-MS), as an efficient method to select SNPs for the tolerance of LS challenge, was utilized for SNP typing. Fifty gene segments were amplified based on comparative transcriptomics in our earlier study, a total of 18 511 bp DNA fragments were amplified,and eighty-five SNP markers were found. The frequency of the SNPs was estimated to be 0.46 per 100 base pairs of DNA sequences. The rate of the conversion mutation was 81%, while the transversion mutation was 19%. The mutation rate of the G/T(C/A), A/T and G/C was 26%, 12% and 7%, respectively. Eight SNP markers were found to significantly correlate with the adaption of low salinity. Of the eight SNP markers, three linked-SNPs were found in the cuticle proportion gene, and another three SNPs were found in three new genes, and the rest two were found in aquaporin gene and chloride channel gene. The development of these SNP markers found in our study could be primarily used for breeding LS-resistant strains of P. trituberculatus. | Yanyan Feng Dening Zhang Jianjian Lv Baoquan Gao Jian Li Ping Liu | 2019 | Acta Oceanologica Sinica2019，38，8： | 0 |
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