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| 1 | Efficient generation of transgenic chickens using the spermatogonial stem cells in vivo and ex vivo transfection显示文摘The highly efficient novel methods to produce transgenic chickens were established by directly in-jecting the recombinant plasmid containing green fluorescent protein (GFP) gene into the cock's testis termed as testis-medianted gene transfer (TMGT), and transplanting transfected spermatogonial stem cells (TTSSCs). For the TMGT approach,four dosages of pEGFP-N1 DNA/cationic polymer complex were injected intratesticularly. The results showed: (1) 48 h after the injection,the percentages of testis cells expressing GFP were 4.0%, 8.7%, 10.2% and 13.6% in the 50, 100, 150 and 200 μg/mL group, re-spectively. The difference from the four dosage groups was significant (P<0.05). On day 25 after the injection, a dosage-dependent and time-dependent increase in the number of transgenic sperm was observed. The percentages of gene expression reached the summit and became stable from day 70 to 160, being 12.7%, 12.8%, 15.9% and 19.1%, respectively. The difference from the four dosage groups was also significant (P<0.05). (2) 70 d after the injection, strong green fluorescent could be observed in the seminiferous tubules by whole-mount in-situ hybridization. (3) 70 d after the injection, the semen was collected and used to artificially inseminate wild-type females. The blastoderms of F1 and F2 transgenic chicken expressed GFP were 56.2% (254/452) and 53.2% (275/517), respectively. The detec-tion of polymerase chain reaction (PCR) of F1 and F2 transgenic chicken blood genomic DNA showed that 56.5% (3/23) of F1 and 52.9% (9/17) of F2 were positive. Southern blot showed GFP DNA was in-serted in their genomic DNAs. (4) Frozen whole mount tissue sections of F1 and F2 transgenic chicken liver, heart, kidney and muscle showed that the rates of green fluorescent positive were between 50.0% and 66.7%. (5) With the TTSSCs method, SSCs ex vivo transfected with GFP were transplanted into recipient roosters whose endogenic SSCs had been resoluted. The donor SSCs settled and GFP ex-pression became readily detectable in the frozen whole mount tissue sections of recepient testes. Moreover, sperms carrying GFP could be produced normally. The results of artificially inseminating wild-type females with these sperms showed 12.5% (8/64) of offspring embryo expressed GFP and 11.1% (2/18) hatched chicks were tested transgenic. Our data therefore suggest TMGT and TTSSCs are the feasible methods for the generation of transgenic chickens. | LI BiChun SUN GuoBo SUN HuaiChang XU Qi GAO Bo ZHOU GuanYue ZHAO WenMing WU XinSheng BAO WenBin YU Fei WANG KeHua CHEN GuoHong | 2008 | Science China(Life Sciences)2008，51，8： | 13 |
| 2 | EMTP-type Program Realization of Krylov Subspace Based Model Reduction Methods for Large-Scale Active Distribution Network显示文摘Effective model reduction methods are required to deal with new challenges in active distribution network simulations that are on a large scale and have complicated structures.In the development of advanced electromagnetic transient simulation programs,automated model reduction plays an important role.This paper proposes an automated realization algorithm for the Krylov subspace based model reduction methods of an active distribution network with which the reduced model can be automatically established according to a given threshold of reduction error.The combined state-space nodal analysis framework is employed to apply the automated model reduction algorithm in popular EMTP-type simulation programs.Simulations are performed using PSCAD and a self-developed program to show the feasibility and validity of the proposed methods. | Chengshan Wang Hao Yu Peng Li Jianzhong Wu Chengdi Ding Guanyu Song Dun Lin Feng Xing | 2015 | CSEE Journal of Power and Energy Systems2015，1，1： | 8 |
| 3 | Radiation-pressure-driven mechanical oscillations in silica microdisk resonators on chip显示文摘We demonstrate radiation-pressure-driven mechanical oscillations from high optical quality factor silica microdisk resonators on chip. Mechanical quality factors of 3520 in air and 12540 in vacuum for the fundamental radial breathing modes are obtained from 73 μm-diameter silica microdisks with mechanical frequencies of ~51 MHz. The measured mechanical oscillation threshold powers for the input light are determined to be 62.5 μW in air and down to 26.6 μW in vacuum. | WANG GuanZhong ZHAO MingMing MA JiYang LI GuanYu CHEN Yuan JIANG XiaoShun XIAO Min | 2015 | Science China(Physics,Mechanics & Astronomy)2015，58，5： | 6 |
| 4 | A direct H2O2 production based on hollow porous carbon sphere-sulfur nanocrystal composites by confinement effect as oxygen reduction electrocatalysts显示文摘Carbon-sulfur composites have draw n in creasing interest in various fields including electrocatalysis because of their unique structures.However,carb on-sulfur composite with tiny sulfur nano crystals has still received little attention.Herein,hollow porous carb on sphere-sulfur composite(HPCS-S)which possesses excellent electrochemical performance towards H2O2 has been prepared for the first time via a simple silica template method.The 2-5 nm sulfur nan ocrystals being restricted in the cha nnel of the hollow porous carb on spheres are un der a strong compressive stress,which was further con firmed by high-resoluti on tran smissi on electr on microscopy(HRTEM)and GPA.The HPCS-S nano crystals show better con ductivity tha n amorphous sulfur clusters because of the reducti on of the steric hindrance which efficie ntly promotes the electron transportation.Consequently,the higher activity and selectivity towards the 2e^oxygen reduction reaction(ORR)to H2O2 in alkaline solution was obtained.The H2O2 selectivity rises from 20%to over 70%after the sulfur addition and the H2O2 production rate achieves 183.99 mmol-gcataiyst-1 with the Faradaic efficiency of 70%.Furthermore,performance enhancement mechanism was also investigated using the den sity functional theory(DFT)calculatio ns.After the in troduci ng of sulfur nano crystals,the appeara nee of S-S bond greatly decreases the overpotential compared with S-doping,which results in significant enhancement of the electrocatalytic property.Consequently,the HPCS-S can be an efficient H2O2 production electrocatalyst in alkaline solution. | Guanyu Chen Jiwei Liu Qingqing Li Pengfei Guan Xuefeng Yu Linshen Xing Jie Zhang Renchao Che | 2019 | Nano Research2019，12，10： | 6 |
| 5 | Advances in lithium niobate photonics:development status and perspectives显示文摘Lithium niobate(LN)has experienced significant developments during past decades due to its versatile properties,especially its large electro-optic(EO)coefficient.For example,bulk LN-based modulators with high speeds and a superior linearity are widely used in typical fiber-optic communication systems.However,with everincreasing demands for signal transmission capacity,the high power and large size of bulk LN-based devices pose great challenges,especially when one of its counterparts,integrated silicon photonics,has experienced dramatic developments in recent decades.Not long ago,high-quality thin-film LN on insulator(LNOI)became commercially available,which has paved the way for integrated LN photonics and opened a hot research area of LN photonics devices.LNOI allows a large refractive index contrast,thus light can be confined within a more compact structure.Together with other properties of LN,such as nonlinear/acousto-optic/pyroelectric effects,various kinds of high-performance integrated LN devices can be demonstrated.A comprehensive summary of advances in LN photonics is provided.As LN photonics has experienced several decades of development,our review includes some of the typical bulk LN devices as well as recently developed thin film LN devices.In this way,readers may be inspired by a complete picture of the evolution of this technology.We first introduce the basic material properties of LN and several key processing technologies for fabricating photonics devices.After that,various kinds of functional devices based on different effects are summarized.Finally,we give a short summary and perspective of LN photonics.We hope this review can give readers more insight into recent advances in LN photonics and contribute to the further development of LN related research. | Guanyu Chen Nanxi Li Jun Da Ng Hong-Lin Lin Yanyan Zhou Yuan Hsing Fu Lennon Yao Ting Lee Yu Yu Ai-Qun Liu Aaron J.Danner | 2022 | Advanced Photonics2022，4，3： | 6 |
| 6 | Influence of white etching layer on rolling contact behavior at wheel-rail interface显示文摘The existence of narrow and brittle white etching layers(WELs)on the rail surface is often linked with the formation of rail defects such as squats and studs,which play the key roles in rail surface degradation and tribological performance.In the present study,a systematic investigation on stress/strain distribution and fatigue life of the WEL during wheel-rail rolling contact was conducted based on a numerical model considering the realistic wheel geometry.This is the first study considering the influence of rail materials,loading pressure,frictional condition,WEL geometry(a/b),and slip ratio(Sr)in the practical service conditions at the same time.The results revealed much higher residual stress in WEL than in rail matrix.Stress changes along the rail depth matched with the previously reported microstructure evolutions.The current work revealed that the maximum difference in contact stress between the wheel passages of rail matrix and the WEL region(noted as stress variation)rises with the increase of loading pressure,the value of a/b,and Sr;but drops with the friction coefficient(μ).In addition,a critical length–depth ratio of 5 for a/b has been found.The fatigue parameter,FP,of the WEL decreased quickly with the length–depth ratio when it was less than 5 and then increased slightly when it was larger than 5.This study also revealed that the fatigue life of the WEL was reduced for high strength head hardened(HH)rail compared with standard carbon(SC)rail. | Qinglin LIAN Guanyu DENG Hongtao ZHU Huijun LI Xi WANG Zhiming LIU | 2020 | Friction2020，8，6： | 4 |
| 7 | Synthesis,structure and optical properties of novel thermally robust Dy^(3+)-doped Ca_(9)Sc(PO_(4))_(7) phosphors for NUV-excited white LEDs显示文摘The powder samples of Ca_(9)Sc(PO_(4))_(7):xDy^(^(3+))white emitting phosphors were prepared via a solid state reaction technique.The Ca_(9)Sc(PO_(4))_(7):Dy^(3+)samples were researched by using the GSAS Rietveld refinement and X-ray diffraction(XRD) methods,and SEM images and elemental maps were recorded.Under 350 nm excitatio n,the emission spectra of Ca_(9)Sc(PO_(4))_(7):xDy^(3+)samples have two obvious peaks and one weak peak at 484,572 and660 nm,corresponding to the characteristic electron transitions of(^(4)F_(9/2)→ ^(6)H_(15/2),blue),(^(4)F_(9/2)→ ^(6)H_(13/2),yellow) and(^(4)F_(9/2)→ 6 H11/2,red),respectively.The concentration quenching effect,decay lifetime and thermal quenching of the as-synthesized Ca_(9)Sc(PO_(4))_(7):Dy^(3+)samples were researched systematically.The Ca_(9)Sc(PO_(4))_(7):0.02 Dy^(3+)phosphor possesses a good thermal stability,of which the emission intensity at 423 K can maintain 79% of the initial value(273 K).In addition,through the study of the chro maticity coordinates of the Ca_(9)Sc(PO_(4))_(7):0.02 Dy^(3+)phosphor,it is found that it is located in the white region,and the Commission Internationalede L’Eclairage(CIE) chromaticity coordinates are(0.339,0.389),The above results show that Ca_(9)Sc(PO_(4))_(7):xDy^(3+)phosphors can be excellent candidate material for applications in NUV-excited white LEDs. | Haiyan Wu Huimin Li Lihong Jiang Ran Pang Su Zhang Da Li Guanyu Liu Chengyu Li Jing Feng Hongjie Zhang | 2021 | Journal of Rare Earths2021，39，3： | 4 |
| 8 | High-Q silica microdisk optical resonators with large wedge angles on a silicon chip显示文摘We experimentally demonstrate high optical quality factor silica microdisk resonators on a silicon chip with large wedge angles by reactive ion etching. For 2-μm-thick microresonators, we have achieved wedge angles of 59°, 63°,70°, and 79° with optical quality factors of 2.4 × 10~7, 8.1 × 10~6, 5.9 × 10~6, and 7.4 × 10~6, respectively, from ~80 μm diameter microresonators in the 1550 nm wavelength band. Also, for 1-μm-thick microresonators, we have obtained an optical quality factor of 7.3 × 10~6 with a wedge angle of 74°. | Guanyu Li Pei Liu Xiaoshun Jiang Chao Yang Jiyang Ma Hongya Wu Min Xiao | 2015 | Photonics Research2015，3，5： | 2 |
| 9 | Control Flow Obfuscation Based Protection Method for Android Applications显示文摘With the popularization and rapid development of mobile intelligent terminals(MITs), the number of mobile applications, or apps, has increased exponentially. It is increasingly common for malicious code to be inserted into counterfeit apps, which can cause significant economic damage and threaten the security of users. Code obfuscation techniques are a highly efficient group of methods for code security protection. In this paper, we propose a novel control flow obfuscation based method for Android code protection. First, algorithms to insert irrelevant code and flatten the control flow are employed that minimize the cost of obfuscation while ensuring its strength. Second, we improve the traditional methods of control flow flattening to further reduce the costs of obfuscation. Lastly, the use of opaque predicates is strengthened by establishing an access control strategy, which converts the identification of opaque predicates in the entire program into a graph traversal problem, and thereby increases the strength of the code protection. We did some experiments to evaluate our method, and the results show that the proposed method can work well. | Yong Peng Guanyu Su Bin Tian Maohua Sun Qi Li | 2017 | China Communications2017，14，11： | 2 |
| 10 | AC loss and contact resistance in highly flexible rebco cable for fusion applications显示文摘High Temperature Superconductor(HTS)materials can operate at higher magnetic fields up to 20 T with high critical current and higher operating temperature,compared to low temperature superconductors(LTS).A Highly Flexible REBCO Cable(HFRC)is introduced at the Institute of Plasma Physics,Chinese Academy of Sciences(ASIPP);a cabling method that is suitable for REBCO HTS tape having anisotropic material properties in its thin REBCO layer.This type of HTS superconducting cable shows high potential for applications in nuclear fusion.The alternating currents and magnetic fields in tokamak type of fusion magnets,cause AC power losses in such cables,which can provoke instability of the conductor by induced currents and increase the temperature.As a first step in characterizing the electromagnetic(EM)performance of an HFRC cable,the AC loss and contact resistance of the HFRC prototype cable were measured at the University of Twente.The measurements were done in liquid helium(4.2 K)with AC magnetic fields,applied perpendicular to the cable's long axis.The AC loss was measured simultaneously by a calibrated gas flow calorimeter utilizing the helium boil-off method,and by the magnetization method using pick-up coils.For the applied test conditions,no coupling loss could be distinguished as a part of the overall AC loss.It is suggested that this might be explained by the shielding of the conductor interior from the applied magnetic field by the outer tape layer due to the high critical current density of the REBCO tape,leading to a high penetration field. | Zichuan Guo Jinggang Qin R.Lubkemann Keyang Wang Huan Jin Guanyu Xiao Jiangang Li Chao Zhou Arend Nijhuis | 2022 | Superconductivity2022，，2： | 2 |
| 11 | Advances and perspectives in catalytic oxidation of hydrocarbons in liquid phase显示文摘This review article summarizes recent advances in catalytic oxidation of hydrocarbons,especially presents two strategies for activation of C-H bonds or molecular oxygen.Based on our own research results,the applications of the two methods in the oxidation of cyclohexane,toluene and ethyl benzene,etc.are introduced,and the perspectives of the two methods are also discussed. | Zhou Lipeng Yang Guanyu Zhang Wei Sun Zhiqiang Gao Jin Miao Hong Chen Chen Ma Hong Li Xiaoqiang Zhang Qiaohong Wang Feng Tong Xinli Xu Jie | 2007 | Progress in Natural Science:Materials International2007，17，9： | 2 |
| 12 | A Precise Calibration Method on Phase Center of Uplink Antenna Array Considering Its Actual Pointing显示文摘With regard to the inferior techniques and low accuracy of phase center calibration of an antenna array, this paper proposes a new calibration method considering the actual antenna pointing by introducing a precise engineering surveying technique to measure the real state of antennas. First, an industrial photogrammetric system is utilized to obtain the coordinates of points on antenna panels in different postures, and the actual pointing of the mechanical axis is obtained via least-squares fitting. Then, based on this, the coordinates of antenna rotation center are obtained by seeking the intersection of mechanical axes via using the matrix method. Finally, the mechanical axis in arbitrary postures is estimated based on the inverse-angle weighting interpolation method, and the reliable phase center is obtained by moving a fixed length from the projective center along the mechanical axis. An uplink antenna array including three ? 3 m antennas is taken as experimental object, and all photogrammetric coordinate systems are unified by the engineering control network, with each antenna phase center precisely calibrated via the proposed method. The results of electrical signal synthesis indicate that this method can effectively overcome the influence of gravity deformation and mechanical installation error, and enhance the synthetic signal magnitude of the uplink antenna array. | Zongchun LI Zhiyong LU Guanyu ZHANG Bo LIU Qiqiang FENG Yinggang GUO Hua HE | 2020 | Journal of Geodesy and Geoinformation Science2020，3，1： | 2 |
| 13 | Information gene and its information knock-out/knock-in 显示文摘 | ZHANG Guanyu LI Enzhong | 2010 | An International Jonrnal Advances in System Science and Applications2010，10，2： | 1 |
| 14 | Information gene and identification of its information knock-out/knock-in显示文摘 | ZHANG Guanyu LI Enzhang | 2010 | An International Journal Advances in Systems Science and Applications2010，10，2： | 1 |
| 15 | Global optimization of reliability design for large ball mill gear transmission based on the Kriging model and genetic algorithm显示文摘 | Guanyu Zhang Guoqiang Wang Xuefei Li Yunpeng Ren | 2013 | Mechanism and Machine Theory2013，，： | 1 |
| 16 | Information gene and identification of its information Knock-out/Knock-in显示文摘 | ZHANG Guanyu LI Enzhong | 2010 | An International Journal Advances in Systems Science and Applications2010，10，2： | 1 |
| 17 | Specific responses of monoamine neurotransmitters to various acute stressors显示文摘This study determined the composition of histamine,serotonin and dopamine using high performance liquid chromatography and electrochemical detection,and compared the changes in monoamine levels in plasma,the cortex and midbrain of mice exposed to acute stressors,such as blood-drawing stimulation or restraint.Results demonstrated that plasma histamine levels were markedly increased when mice were exposed to blood-drawing stimulation and restraint stress.However,serotonin levels decreased in plasma of mice treated with restraint stress,and dopamine levels in plasma had no significant response to the two acute stressors.The three monoamines (histamine,serotonin and dopamine) increased at different degrees in restraint mice,but not in brain regions of blood-drawing stressed mice.Results indicated that histaminergic,serotonergic or dopaminergic systems have their own specific response to different acute stressors. | Rongrong He Guanyu Lin Yifang Li Keiich Abe Xinsheng Yao Hiroshi Kurihara | 2011 | Neural Regeneration Research2011，6，14： | 1 |
| 18 | Impacts of El Nino on the somatic condition of Humboldt squid based on the beak morphology显示文摘The Humboldt squid Dosidicus gigas has a short life span, and environmental variability plays a significant role in regulating its population dynamics and distribution. An analysis of 1 096 samples of D. gigas collected by the Chinese commercial fishing vessels during 2013, 2014, and 2016 off the Peruvian Exclusive Economic Zone, was conducted to evaluate the impacts of El Nino events on the somatic condition of D. gigas. This study indicates that the slopes of all beak variables in relation to mantle length (ML) for females were greater than those of males during 2013, 2014, and 2016, and slopes of the upper crest length and the lower rostrum length significantly differed between females and males in 2013 (P<0.05). Variation in the slopes for beak variables among years was studied;no significant difference was observed (ANCOVA, P>0.05). The Fulton's condition coefficients (K) of females and males in 2013 and 2014 were significantly greater than those in 2016 (P<0.01). The K values of females were greater than those of males in 2013, 2014, and 2016, and K values significantly differed between females and males in 2013. In normal years, the chlorophyll a (Chl a) concentration showed an N-shaped variability from January to December. However, in the El Nino period, it tended to weaken the upwelling coupled with warm and low Chl a concentration waters. We suggest that the poor somatic condition of D. gigas during the El Nino year was resulted from the low Chl a concentration in the waters, and the abundance of D. gigas would decrease due to the unfavourable environment and the lack of prey items in the El Nino year. | HU Guanyu YU Wei LI Bai HAN Dongyan CHEN Xinjun CHEN Yong LI Jianhua | 2019 | Journal of Oceanology and Limnology2019，37，4： | 1 |
| 19 | Information gene and identification of its information knock-out/knock-in显示文摘 | Zhang Guanyu Li Enzhong | 2010 | An International Journal Advances in Systems Science and Applications2010，10，2： | 1 |
| 20 | Research on technology of traffic video incidents detection under highway condition显示文摘 | Liu Xiaoming Zhang Zhonghui Li Guanyu | 2010 | The Journal of China Universities of Posts and Telecommunications2010，23，17： | 1 |
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