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| 1 | The Yellow Sea green tides were dominated by one species, Ulva (Enteromorpha) prolifera, from 2007 to 2011显示文摘Green tides have occurred in the Yellow Sea successively from 2007 to 2011. Genetic analysis of the 5-year green-tide-forming algae needs to be performed to determine the source of the biomass and understand the mechanism of the green tide blooms. In this study, free-floating green algae were collected at different sites in the Yellow Sea in 2010 and 2011. Data on 182 free-floating samples and 155 attached Ulva samples from previous studies on the Yellow Sea green tides from 2007 to 2009 were also taken into consideration. Morphology observation and molecular phylogenetic analyses indicated that the Yellow Sea green tides were dominated by a single species, Ulva prolifera, from 2007 to 2011. Considering that at least five Ulva species inhabit the north coast of China, the unialgal composition of the green tides implied that (1) there may be some special physiology and propagation pathways of U. prolifera for its rapid expansion, (2) the mechanisms of the Yellow Sea green tide formation were similar for the last five years, and (3) the intra-species genetic variation and population structure of U. prolifera need to be studied to determine the exact origin of the bloom-forming biomass. | ZHAO Jin JIANG Peng LIU ZhengYi WEI Wei LIN HanZhi LI FuChao WANG JinFeng QIN Song | 2013 | Chinese Science Bulletin2013，58，19： | 27 |
| 2 | Combinational biosynthesis and characterization of a fluorescent 82β-phycocyanin of Spirulina platensis显示文摘To biosynthesize fluorescent Spirulina platensis (Sp)β -phycocyanin (PC) in Escherichia coli, a BLASTP search for homologs of the cpeS gene, a chromophore lyase, was performed against the Synechocystis sp. PCC 6803 (S6) proteome. A highly homologous gene, slr2049, was obtained from the S6 genome. Sites 82 and 153 in -phycocyanin of Sp were modified by site-directed muta- genesis. Two recombinant expression vectors were constructed and transformed into E. coli BL21: (i) pCDF-cpcB (C153A)- slr2049-sll0583-ho1-pcyA; and (ii) pCDF-cpcB (C82I)-slr2049-sll0583-ho1-pcyA. Lyases encoded by the genes slr2049 and sll0583 catalyzed the linking of Sp 82β -PC to phycocyanobilin (PCB), and fluorescent CpcB (C153A)-PCB was generated. We present a strategy for the co-expression of multiple genes in a single expression vector to identify the function of an unknown gene. Recombinant phycobiliproteins produced on a large scale are promising fluorescent tags for diagnostics and pharmacology. | GUAN XiangYu ZHANG WeiJie CHI XiaoYuan LIN HanZhi WANG JinFeng QIN Song | 2012 | Chinese Science Bulletin2012，57，25： | 3 |
| 3 | Root-associated(rhizosphere and endosphere) microbiomes of the Miscanthus sinensis and their response to the heavy metal contamination显示文摘The plant root-associated microbiomes, including both the rhizosphere and the root endosphere microbial community, are considered as a critical extension of the plant genome. Comparing to the well-studied rhizosphere microbiome, the understanding of the root endophytic microbiome is still in its infancy. Miscanthus sinensis is a pioneering plant that could thrive on metal contaminated lands and holds the potential for phytoremediation applications. Characterizing its root-associated microbiome, especially the root endophytic microbiome, could provide pivotal knowledge for phytoremediation of mine tailings. In the current study, M. sinensis residing in two Pb/Zn tailings and one uncontaminated site were collected. The results demonstrated that the metal contaminant fractions exposed strong impacts on the microbial community structures. Their influences on the microbial community, however, gradually decreases from the bulk soil through the rhizosphere soil and finally to the endosphere, which resulting in distinct root endophytic microbial community structures compared to both the bulk and rhizosphere soil. Diverse members affiliated with the order Rhizobiales was identified as the core microbiome residing in the root of M. sinensis. In addition, enrichment of plant-growth promoting functions within the root endosphere were predicted, suggesting the root endophytes may provide critical services to the host plant. The current study provides new insights into taxonomy and potential functions of the root-associated microbiomes of the pioneer plant, M. sinensis, which may facilitate future phytoremediation practices. | Xiaoxu Sun Benru Song Rui Xu Miaomiao Zhang Pin Gao Hanzhi Lin Weimin Sun | 2021 | Journal of Environmental Sciences2021，33，6： | 3 |
| 4 | A novel and specific method for the determination of aristolochic acid-derived DNA adducts in exfoliated urothelial cells by using ultra performance liquid chromatography-triple quadrupole mass spectrometry显示文摘 | GUO Lin WU Hanzhi YUE Hao | 2011 | Journal of Chromatography2011，879，2： | 1 |
| 5 | Molecular analysis of green-tide-forming macroalgae in the Yellow Sea显示文摘 | Jinfeng Wang Peng Jiang Yulin Cui Nan Li Mingqing Wang Hanzhi Lin Peimin He Song Qin | 2010 | Aquatic Botany2010，，1： | 1 |
| 6 | Editor's note显示文摘The coastal zone spans both land and water, extending landward up to the highest water line that occurs on shore during storms and seaward to the point at which storm waves no longer move sediments on the seafloor. The specific contact line between land and sea is the shoreline and adjacent land is | QIN Song LIU DongYan LIN HanZhi | 2013 | Chinese Science Bulletin2013，58，19： | 0 |
| 7 | Omics in marine biotechnology显示文摘Marine biotechnology emerged in the 1980s, and since then it has been gradually gaining momentum to meet growing demands that cannot be satisfied by terrestrial sources alone. | Qin Song WATABE Shugo Lin HanZhi | 2012 | Chinese Science Bulletin2012，57，25： | 0 |
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