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| 1 | Uncoupling protein 1 mRNA, mitoehondrial GTP-binding, and T4 5'-deiodinase of brown adipose tissue in euthermic Daurian ground squirrel during cold exposure显示文摘 | LIU Xiaotuan LI Qingfen LIN Qishui | 2001 | Comp Biochem Physiol A2001，128，4： | 1 |
| 2 | Epidermal growth factor induces changes of interaction between epidermal growth factor receptor and actin in intact cells显示文摘表皮的生长因素受体(EGFR ) 是 cyto-skeleton-binding 蛋白质。尽管净化的 EGFR 能与在 vitro 行动交往,通常,至少 10% EGFR 在 A431 细胞的不可溶解的细胞骨架部分存在,当表皮的生长因素在细胞媒介介绍时,有肌动朊的 cytosolic EGFR 的相互作用能被荧光回声精力转移仅仅设想。结果显示在 EGFR 和肌动朊之间的正确取向在信号 transduction 过程是重要的。 | Wei Song Haixing Xuan Qishui Lin | 2008 | Acta Biochimica et Biophysica Sinica2008，40，8： | 1 |
| 3 | Stearylamine liposome as a new efficient reagent for DNA transfection of eukaryotic cells显示文摘 | WANG Die JING Naihe LIN Qishui | 1996 | Biochem Biophy Res Commu1996，226，2： | 1 |
| 4 | Homologous up-regulation of androgen receptor expression by androgen in vascular smooth muscle cells显示文摘 | Ma Rui Wu Saizhu Lin Qishui | 2005 | Hormone Research2005，63，： | 1 |
| 5 | Taurocholic acid inhibits the response to interferon-αtherapy in patients with HBeAg-positive chronic hepatitis B by impairing CD8^(+)T and NK cell function显示文摘Pegylated interferon-alpha (PegIFNα) therapy has limited effectiveness in hepatitis B e-antigen (HBeAg)-positive chronic hepatitis B (CHB) patients. However, the mechanism underlying this failure is poorly understood. We aimed to investigate the influence of bile acids (BAs), especially taurocholic acid (TCA), on the response to PegIFNα therapy in CHB patients. Here, we used mass spectrometry to determine serum BA profiles in 110 patients with chronic HBV infection and 20 healthy controls (HCs). We found that serum BAs, especially TCA, were significantly elevated in HBeAg-positive CHB patients compared with those in HCs and patients in other phases of chronic HBV infection. Moreover, serum BAs, particularly TCA, inhibited the response to PegIFNα therapy in HBeAg-positive CHB patients. Mechanistically, the expression levels of IFN-γ, TNF-α, granzyme B, and perforin were measured using flow cytometry to assess the effector functions of immune cells in patients with low or high BA levels. We found that BAs reduced the number and proportion and impaired the effector functions of CD3^(+)CD8^(+) T cells and natural killer (NK) cells in HBeAg-positive CHB patients. TCA in particular reduced the frequency and impaired the effector functions of CD3^(+)CD8^(+) T and NK cells in vitro and in vivo and inhibited the immunoregulatory activity of IFN-α in vitro. Thus, our results show that BAs, especially TCA, inhibit the response to PegIFNα therapy by impairing the effector functions of CD3^(+)CD8^(+) T and NK cells in HBeAg-positive CHB patients. Our findings suggest that targeting TCA could be a promising approach for restoring IFN-α responsiveness during CHB treatment. | Zhen Xun Jinpiao Lin Qingqing Yu Can Liu Jinlan Huang Hongyan Shang Jianhui Guo Yuchen Ye Wennan Wu Yongbin Zeng Songhang Wu Siyi Xu Tianbin Chen Jing Chen Qishui Ou | 2021 | Cellular & Molecular Immunology2021，18，2： | 1 |
| 6 | HBV serum markers of 49164 patients and their relationships to HBV genotype in Fujian province of China显示文摘 | Sheng Lin Can Liu Hongyan Shang Huijuan Chen Bin Yang Jing Chen Yong Chen Dan Chen Qishui Ou | 2013 | J Clin Lab Anal2013，，2： | 1 |
| 7 | Evaluation of the performance of four methods for detection of hepatitis B surface antigen and their application for testing 116,455 specimens显示文摘 | Can Liu Tianbin Chen Jinpiao Lin Huijuan Chen Jing Chen Sheng Lin Bin Yang Hongyan Shang Qishui Ou | 2014 | Journal of Virological Methods2014，，： | 1 |
| 8 | cDNA cloning, functional expression and cellular localization of rat liver mitochondrial electron-transfer flavoprotein-ubiquinone oxidoreductase protein显示文摘A membrane-bound protein was purified from rat liver mitochondria. After being di-gested with V8 protease, two peptides containing identical 14 amino acid residue sequences were obtained. Using the 14 amino acid peptide derived DNA sequence as gene specific primer, the cDNA of correspondent gene 5′-terminal and 3′-terminal were obtained by RACE technique. The full-length cDNA that encoded a protein of 616 amino acids was thus cloned, which included the above mentioned peptide sequence. The full length cDNA was highly homologous to that of human ETF-QO, indicating that it may be the cDNA of rat ETF-QO. ETF-QO is an iron sulfur protein located in mitochondria inner membrane containing two kinds of redox center: FAD and [4Fe-4S] center. After comparing the sequence from the cDNA of the 616 amino acids protein with that of the mature protein of rat liver mitochondria, it was found that the N terminal 32 amino acid residues did not exist in the mature protein, indicating that the cDNA was that of ETF-QOp. When the cDNA was expressed in Saccharomyces cerevisiae with inducible vectors, the protein product was enriched in mitochondrial fraction and exhibited electron transfer activity (NBT re-ductase activity) of ETF-QO. Results demonstrated that the 32 amino acid peptide was a mito-chondrial targeting peptide, and both FAD and iron-sulfur cluster were inserted properly into the expressed ETF-QO. ETF-QO had a high level expression in rat heart, liver and kidney. The fu-sion protein of GFP-ETF-QO co-localized with mitochondria in COS-7 cells. | HUANG Shengbing SONG Wei LIN Qishui | 2005 | Science China(Life Sciences)2005，48，4： | 0 |
| 9 | Non-radioisotopic method for the in vitro measurement of EGF receptor tyrosine kinase显示文摘A non-radioisotopic method was developed for the assay of epidermal growth factor receptor (EGFR). A peptide with twenty amino acid residues around Tyr 1173, the major phosphorylation site of EGFR, was cloned as a GST fusion protein and used as substrate. Anti-phosphoty-rosine monoclonal antibody PY99 was used for the determination of the extent of phosphorylation. Both the specificity and the sensitivity were substantially higher than that of the existing method. Km value of the fusion protein is much lower (10 μmol/L) than that of the synthetic peptide (110 μmol/L). The method can be applied to the measurement of the tyrosine kinase activity of c-erb B2 (Neu/HER2). | Gaoxiang Ge Jing Wu Qishui Lin | 2001 | Chinese Science Bulletin2001，46，8： | 0 |
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