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| 1 | Nitric oxide from mesenchymat stem cells suppressing T - cell proliferation inhibited expression of T- bet in T cells显示文摘 | LIU GEXIU HE DONGMEI TAN GUANGXIAO | 2007 | Blood2007，110，11： | 1 |
| 2 | Down-Regulation of Bcl-2 Protein Sensitizes NCI-H460 Cells to Radiotherapy-Induced Apoptosis显示文摘OBJECTIVE To determine whether Bcl -2 protein down -regulation can render NCI -460 cells more susceptible to gamma radiation -induced apoptosis by treatment with antisense oligonucleotide (ASODN) against the coding region of Bcl-2 mRNA. METHODS Cell survival was determined using the trypan blue dye exclusion. Expression of the Bcl-2 protein was assayed using immunofluo-rescence labeling with fluoresce isothiocyanate. Apoptosis was determined by Giemsa staining and flow cytomertry. RESULTS It was found that Bcl-2 ASODN combined with radiation significantly reduced the number of viable cells (P<0.05). There was no difference in cell survival between a nonsense oligodeoxynucleotide/radia-tion combination and cells treated with radiation alone. Bcl -2 ASODN combined with radiation significantly inhibited expression of the Bcl -2 protein in the NCI-H460 cells (P<0.05). Using Giemsa staining, cells treated with Bcl -2 ASODN combined with radiation at 72 h displayed classic apoptotic changes. Apoptotic rates of the NCI-H460 cells treated with Bcl-2 ASODN combined with radiation significantly increased (P< 0.05), compared with either a nonsense oligodeoxynucleotide/radiation combination or radiation-treatment cells alone. CONCLUSION ASODN against the coding region of Bcl-2 mRNA increases radiation-induced apoptosis in NCI-H460 cells. | Dongmei He Yuan Zhong Gexiu Liu | 2006 | Chinese Journal of Clinical Oncology2006，3，2： | 0 |
| 3 | Bcl-2 Small Interfering RNA Inhibits the Growth of Human Lymphoma Transplanted Subcutaneously in Nude Mice显示文摘OBJECTIVE To investigate whether small hairpin RNA (shRNA)targeting Bcl-2 mRNA could inhibit the growth of lymphomatransplanted subcutaneously in nude mice.METHODS Recombinant Bcl-2 shRNA expression vector withgreen fluorescence protein (GFP) gene was constructed andpreserved in our lab. We evaluated the antitumor effect of the Bcl-2shRNA in vivo which was the model of nude mice bearing Rajicells xenografts. Human Raji cells were injected subcutaneouslyinto nude mice to establish lymphoma models. When thediameters of tumor were above 0.5 cm after Raji cells injection,the mice bearing tumor were randomly divided into four groups:saline control group, negative shRNA group, plasmid vectorgroup, Bcl-2 shRNA group. The polyethylenimine (PEI) was usedto transfect shRNA into tumor. The mixed PEI and shRNA wasinjected into tumors. The growth and size of tumor were observed.Tissue was stained by H&E for its pathological morphology. Theexpression of Bcl-2 mRNA in the tumor mass was detected byreverse transcription polymerase chain reaction (RT-PCR).RESULTS A significant difference in median tumor weightwas observed in mice treated with Bcl-2 shRNA, compared withthose in the groups of negative shRNA or plasmid vector or salinesolution (P< 0.05). Pathological evaluation was completed in allexcised tumors from nude mice bearing Raji cells xenografts.The tumor tissue of the mice treated with Bcl-2 shRNA showedapoptosis, serious necrosis of the cells and inflammatory cellsinfiltration. There was no change in the morphology of cellsamong negative shRNA, plasmid vector and saline solution group.In the group of the Bcl-2 shRNA, the expression levels of Bcl-2mRNA of the tumor tissue were effectively inhibited (P < 0.05).CONCLUSION The shRNA targeting at the Bcl-2 mRNAcould inhibit the growth of human lymphoma transplantedsubcutaneously in nude mice. | Dongmei He Baoying Fang Yangqiu Li Gexiu Liu Yuan Zhang | 2009 | Chinese Journal of Clinical Oncology2009，6，1： | 0 |
| 4 | Retinoic acid enhances expression of neural specific genes in Sca-1^+ cells of mouse fetal liver through activating protein kinase C显示文摘BACKGROUND: Interstitial stem cell is characterized by multiple differentiations, and retinoic acid (RA) can induce differentiation of stromal cells into nerve tissue cells in fetal liver of mice, so, its signal transduction pathway should be discussed to trigger differentiation. OBJECTIVE: To study the effect of RA on expression of neural specific gene and its signal transduction in fetal liver of mice. DESIGN: Paired controlled study on the basis of cell. SETTING: Institute of Hematology, Medical College of Jinan University. MATERIALS: The experiment was completed in the Institute of Hematology, Medical College of Jinan University from April to December 2005. C57BL/6 mice, of clean grade, aged 8-10 weeks, weighting 20-35 g, 10 females and 4 males, were selected in this study. METHODS: Sca-1+ cells in fetal liver were prepared with MACS kit and cultured with DMEM + 10% fetal bovine serum (FBS). On the fourth day, it was added with or without protein kinase C (PKC) inhibitor chelerythrine chloride (3 μmol/L) and 5×10-7 mol/L RA for 24 hours, and then incubated in serum-free medium for 5 days. Expressions of genes were assayed by Western blotting and semi-quantitative reverse transcription polymerase chain reaction. MAIN OUTCOME MEASURES: Expression of neural specific gene NF-L, NF-H, BF-1 and TH. RESULTS: Expression of neural specific gene NF-L, NF-H, BF-1 and TH was significantly increased after treatment with RA and they were increased 5.06, 5.15, 4.63 and 3.33 times, respectively. However, chelerythrine chloride could inhibit expression of neural specific gene NF-L, NF-H, BF-1 and TH induced by RA. CONCLUSION: RA can promote the expression of neural specific genes in Sca-1+ cells of fetal liver, and its pathway may be related to PKC. | Gexiu Liu Yuan Zhang Dongmei He | 2006 | Neural Regeneration Research2006，1，1： | 0 |
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