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| 1 | CRISPR/Cas9-mediated PINK1 deletion leads to neurodegeneration in rhesus monkeys显示文摘Dear Editor,PINK1 mutations cause autosomal recessive and early-onset Parkinson’s disease(PD)with selective neurodegeneration.Unfortunately,current PINK1 knockout(KO)mouse1,2 and pig models3,4 are unable to recapitulate the selective and overt neurodegeneration seen in PD patients.Furthermore,endogenous Pink1 in the mouse brain is expressed at very low levels and can only be detected via immunoprecipitation,5 meaning that PINK1’s function in the mammalian brain needs to be assessed using larger animals that are closer to humans.We previously used CRISPR/Cas9 to target the monkey gene in one-cell stage embryos.6 Using the same approach,we designed two gRNAs to target exon 2(T1)and exon 4(T2),which encode a kinase domain in the PINK1 gene of rhesus monkeys(Fig.1a).CRISPR/Cas9 and PINK1 gRNAs were injected into one-cell stage rhesus monkey embryos.A T7E1 assay and sequencing of PCR products from the injected embryos showed high efficiency(61.5%)in targeting PINK1(Fig.1b and Supplementary information,Fig.S1).Transfer of 87 embryos to 28 surrogate rhesus monkeys resulted in 11 pregnancies(39.2%)(Fig.1b).Eleven fetuses developed to term and were born naturally.Of these live monkeys,eight carried PINK1 mutations(M),and three were wild type(WT).However,three mutant monkeys(M1,M3 and M4)were newborn triplets that struggled to survive and died 3-4 days after birth.One WT newborn monkey also died after a difficult labor.Another mutant monkey(M2)died 7 days after birth without noticeable warning signs or symptoms.The other three mutant monkeys(M6,M7 and M8)have lived for three years;M5,however,reduced its food intake and showed weakness at the age of 1.5 years,and died 30 days after anesthesia for MRI examination. | Weili Yang Yunbo Liu Zhuchi Tu Chong Xiao Sen Yan Xishan Ma Xiangyu Guo Xiusheng Chen Peng Yin Zhengyi Yang Su Yang Tianzi Jiang Shihua Li Chuan Qin Xiao-Jiang Li | 2019 | Cell Research2019，29，4： | 12 |
| 2 | On the Structure of Cyclic Codes over F_q+uF_q+vF_q+uvF_q显示文摘In this paper,cyclic codes over the ring R=F4+uF4+vF4+uvF4 are discussed where the ring R is not a finite chain ring.By studying the polynomial ring Rn=(F4+uF4+vF4+uvF4) and using the corresponding relationship between the cyclic codes in R and the ideals in,cyclic codes over the ring R are characterized.Finally,a Gray-map is obtained and the image of cyclic codes in R is characterized. | XU Xiaofang LIU Xiusheng | 2011 | Wuhan University Journal of Natural Sciences2011，16，5： | 5 |
| 3 | PINK1 kinase dysfunction triggers neurodegeneration in the primate brain without impacting mitochondrial homeostasis显示文摘In vitro studies have established the prevalent theory that the mitochondrial kinase PINK1 protects neurodegeneration by removing damaged mitochondria in Parkinson's disease(PD).However,difficulty in detecting endogenous PINK1 protein in rodent brains and cell lines has prevented the rigorous investigation of the in vivo role of PINK1.Here we report that PINK1 kinase form is selectively expressed in the human and monkey brains.CRISPR/Cas9-mediated deficiency of PINK1 causes similar neurodegeneration in the brains of fetal and adult monkeys as well as cultured monkey neurons without affecting mitochondrial protein expression and morphology.Importantly,PINK1 mutations in the primate brain and human cells reduce protein phosphorylation that is important for neuronal function and survival.Our findings suggest that PINK1 kinase activity rather than its mitochondrial function is essential for the neuronal survival in the primate brains and that its kinase dysfunction could be involved in the pathogenesis of PD. | Weili Yang Xiangyu Guo Zhuchi Tu Xiusheng Chen Rui Han Yanting Liu Sen Yan Qi Wang Zhifu Wang Xianxian Zhao Yunpeng Zhang Xin Xiong Huiming Yang Peng Yin Huida Wan Xingxing Chen Jifeng Guo Xiao-Xin Yan Lujian Liao Shihua Li Xiao-Jiang Li | 2022 | Protein & Cell2022，13，1： | 3 |
| 4 | China's adjustment of climate change policies:what lessons can be learned from the US?显示文摘From the US New Energy Plan, personnel appointment, and diplomatic activities, etc., we can see that US is now on the way to returning to the negotiation table and undertaking the leadership in addressing climate change. What US has done puts tremendous challenges on China, which emits large amount of greenhouse gas into the atmosphere. However, different from US, China is undergoing rapid development, and huge energy consumption is unavoidable. China has to make a balance between developing economy and addressing climate change. This paper focuses on the US climate change policies and its greenhouse gas emissions, and its influences on China climate change policy. China greenhouse gas emissions and relative policies are also analyzed to see what situation China is in, and what challenges that China is facing. Consequently, combing China's local situation, we propose several solutions for China to address climate change, i.e. moving towards a low carbon economy, struggling for emitting more, enhancing China-US cooperation, and implementing different climate change policies based on local situation. Meanwhile, the achievements that China has made are also introduced. | Liu Mingming Zhao Xiusheng | 2009 | Ecological Economy2009，5，3： | 2 |
| 5 | Design and application of an air-cooled probe for total pressure measurement at combustor outlet显示文摘An air-cooled probe was designed for measurement of total pressure at combustor outlet;its cooling scheme combined the film cooling and convection cooling.With the aid of CFD technique,cooling effectiveness of the coolant jets at various blow ratios was compared;suitable blow ratios and configuration of film holes were chosen accordingly.The overall cooling performance of the probe was evaluated via CFD technique,the design was improved according to the simulation result,and the cooling effect of the leading edge was obviously strengthened by increasing the local coolant mass flow rate.The results of wind tunnel test indicated that,between Mach numbers 0.2 and 0.4,the probe achieved a high accuracy at various attack angles.The probe was utilized in an annular combustor rig test,the highest temperature reached 1 760 Kand total pressure reaches 1 036 kPa.The result of rig test demonstrates that the coolant film distribution consistent appropriately with the CFD results. | LIU Xupeng SUN Qi WANG Xiaoliang XUE Xiusheng YIN Dong | 2019 | 航空动力学报2019，34，1： | 1 |
| 6 | Recent progress in all-inorganic metal halide nanostructured perovskites:Materials design,optical properties,and application显示文摘Low-dimensional all-inorganic metal halide perovskite(AIMHP)materials,as a new class of nanomaterials,hold great promise for various optoelectronic devices.In the past few years,tremendous progress has been achieved in the development of efficient and stable AIMHP nanomaterials for optical property studies and related applications.Here,we offer a critical overview on the unique merits and the state-of-the-art design of AIMHP using different composition strategies.Then,the effects of material compositions,dimensionality,morphologies and structures on optical properties are summarized.We also comprehensively present recent advances in the development AIMHP nanomaterials for practical applications including solar cells,light-emitting diodes,lasers and photodetectors.Lastly,the critical challenges and future opportunities in this emerging field are highlighted. | Lianzhen Cao Xia Liu Yingde Li Xiusheng Li Lena Du Shengyao Chen Shenlong Zhao Cong Wang | 2021 | Frontiers of physics2021，16，3： | 1 |
| 7 | Properties of Low Surface Energy Fluorocarbon Polymers with Fluoro-acrylic Resins显示文摘 | Liu Xiusheng Wang Can Liu Lanxuan | 2008 | Journal of Wuhan University of Technology:Mater2008，，1： | 1 |
| 8 | Enumeration of Generalized Linear Orthomorphism in the Vector Space F_q^n显示文摘The conception of orthomorphism has been general-ized in this paper, and a counting formula on the generalized linear orthomorphism in the vector space over the Galois field with the arbitrary prime number p as the characteristic is obtained. Thus, the partial generation algorithm of generalized linear orthomorphism is achieved. The counting formula of the linear orthomor-phism in the vector space over the finite field with characteristic 2 is the special case in our results. Furthermore, the generalized linear orthomorphism generated and discussed in this paper can gain the maximum branch number when they are designed as P-permutations. | HAN Haiqing LI Qin TONG Yan LIU Xiusheng | 2012 | Wuhan University Journal of Natural Sciences2012，17，1： | 0 |
| 9 | On the Arithmetic of Endomorphism Ring End (Zp×Zpm)显示文摘 | LIU Xiusheng LIU Hualu HU Peng | 2018 | Wuhan University Journal of Natural Sciences2018，23，4： | 0 |
| 10 | Three New Classes of Subsystem Codes显示文摘In this paper,we construct three classes of Clifford subsystem maximum distance separable(MDS)codes based on Reed-Solomon codes and extended generalized Reed-Solomon codes over finite fields Fq for specific code lengths.Moreover,our Clifford subsystem MDS codes are new because their parameters differ from the previously known ones. | LI Hui LIU Xiusheng HU Peng | 2024 | Wuhan University Journal of Natural Sciences2024，29，1： | 0 |
| 11 | Optimal Asymmetric Quantum Codes from the Euclidean Sums of Linear Codes显示文摘In this paper,we first give the definition of the Euclidean sums of linear codes,and prove that the Euclidean sums of linear codes are Euclidean dual-containing.Then we construct two new classes of optimal asymmetric quantum error-correcting codes based on Euclidean sums of the Reed-Solomon codes,and two new classes of optimal asymmetric quantum error-correcting codes based on Euclidean sums of linear codes generated by Vandermonde matrices over finite fields.Moreover,these optimal asymmetric quantum errorcorrecting codes constructed in this paper are different from the ones in the literature. | XU Peng LIU Xiusheng | 2024 | Wuhan University Journal of Natural Sciences2024，29，1： | 0 |
| 12 | Macwilliams Identities of Linear Codes over the Ring F_2+ uF_2+ vF_2显示文摘The Lee weight enumerators and the complete weight enumerators for the linear codes over ring R = F2 + u F2 + v F2 are defined and Gray map from Rnto F3n2 is constructed. By proving the fact that the Gray images of the self-dual codes over R are the self-dual codes over F2, and based on the Mac Williams identities for the Hamming weight enumerators of linear codes over F2, the Mac Williams identities for Lee weight enumerators of linear codes over R are given. Further, by introducing a special variable t, the Mac Williams identities for the complete weight enumerators of linear codes over R are obtained. Finally, an example which illustrates the correctness and function of the two Mac Williams identities is provided. | LIU Xiusheng LIU Hualu | 2015 | Journal of Systems Science & Complexity2015，28，3： | 0 |
| 13 | Constacyclic Codes of Length Ps over Finite Rings FPm+uFPm+vFPm+uvFPm显示文摘Let R-Fpm+uFpm+vFpm+uvFpm,where u2=v2=0,uv=vu.Then R is a local ring,but it is not a chain ring.R contains precisely(pm-1)p3 m units,namely,α+uβ+vγ+uvδ,where α,β,γ,δ∈Fpm,α≠0.In this paper,we investigate all constacyclic codes of length ps over R.Firstly,we classify allα-constacyclic and(α+uvβ)-constacyclic codes of length ps over R,respectively,and obtain their structure in each of thoseα-constacyclic and(α+uvβ)-constacyclic codes.Secondly,we address the(α+uβ)-constacyclic codes of length ps over R,and get their classification and structure.Finally,using similar discussion of(α+uβ)-constacyclic codes,we obtain the classification and the structure of α+vβ,α+uβ+uvγ,α+vβ+uvγ,α+uβ+vγ,α+uβ+vβ+uvδ-constacyclic codes of length ps over R. | HU Peng LIU Xiusheng | 2020 | Wuhan University Journal of Natural Sciences2020，25，4： | 0 |
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