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| 1 | Spatial organization of bacterial flora in normal and inflamed intestine: A fluorescence in situ hybridization study in mice显示文摘AIM: To studythe role of intestinal flora in inflammatory bowel disease (IBD).METHODS: The spatial organization of intestinal flora was investigated in normal mice and in two models of murine colitis using fluorescence in situ hybridization.RESULTS: The murine small intestine was nearly bacteriafree. The normal colonic flora was organized in three distinct compartments (crypt, interlaced, and fecal), each with different bacterial compositions. Crypt bacteria were present in the cecum and proximal colon. The fecal compartment was composed of homogeneously mixed bacterial groups that directly contacted the colonic wall in the cecum but were separated from the proximal colonic wall by a dense interlaced layer. Beginning in the middle colon, a mucus gap of growing thickness physically separated all intestinal bacteria from contact with the epithelium. Colonic inflammation was accompanied with a depletion of bacteria within the fecal compartment, a reduced surface area in which feces had direct contact with the colonic wall, increased thickness and spread of the mucus gap, and massive increases of bacterial concentrations in the crypt and interlaced compartments. Adhesive and infiltrative bacteria were observed in inflamed colon only, with dominant Bacteroides species.CONCLUSION: The proximal and distal colons are functionally different organs with respect to the intestinal flora, representing a bioreactor and a Segregation device.The highly organized structure of the colonic flora, its specific arrangement in different colonic segments, and its specialized response to inflammatory stimuli indicate that the intestinal flora is an innate part of host immunity that is under complex control. | Alexander Swidsinski Vera Loening-Baucke Herbert Lochs Laura P.Hale | 2005 | World Journal of Gastroenterology2005，11，8： | 27 |
| 2 | Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum显示文摘The use of periosteum-derived progenitor cells (PCs) combined with bioresorbable materials is an attractive approach for tissue engineering. The aim of this study was to characterize the osteogenic differentiation of PC in 3-dimensional (3D) poly-lactic-co-glycolic acid (PLGA) fleeces cultured in medium containing allogeneic human serum. PCs were isolated and expanded in monolayer culture. Expanded cells of passage 3 were seeded into PLGA constructs and cultured in osteogenic me- dium for a maximum period of 28 d. Morphological, histological and cell viability analyses of three-dimensionally cultured PCs were performed to elucidate osseous synthesis and deposition of a calcified matrix. Furthermore, the mRNA expression of type I collagen, osteocalcin and osteonectin was semi-quantitively evaluated by real-time reverse transcriptase-polymerase chain reac- tion (RT-PCR). The fibrin gel immobilization technique provided homogeneous PCs distribution in 3D PLGA constructs. Live-dead staining indicated a high viability rate of PCs inside the PLGA scaffolds. Secreted nodules of neo-bone tissue formation and the presence of matrix mineralization were confirmed by positive von Kossa staining. The osteogenic differentiation of PCs was further demonstrated by the detection of type I collagen, osteocalcin and osteonectin gene expression. The results of this study support the concept that this tissue engineering method presents a promising method for creation of new bone in vivo. | ZHENG Yi-xiong RINGE Jochen LIANG Zhong LOCH Alexander CHEN Li SITTINGER Michael | 2006 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2006，7，10： | 8 |
| 3 | Mucosal flora in inflammatory bowel disease显示文摘 | Alexander Swidsinski Axel Ladhoff Annelie Pernthaler Sonja Swidsinski Vera Loening-Baucke Marianne Ortner Jutta Weber Uwe Hoffmann Stefan Schreiber Manfred Dietel Herbert Lochs | 2002 | Gastroenterology2002，，1： | 2 |
| 4 | Mucosal flora in inflammatory bowel disease显示文摘 | Alexander Swidsinski Axel Ladhoff Annelie Pernthaler Sonja Swidsinski Vera Loening-Baucke Marianne Ortner Jutta Weber Uwe Hoffmann Stefan Schreiber Manfred Dietel Herbert Lochs | 2002 | Gastroenterology2002，，1： | 1 |
| 5 | Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum显示文摘 | Yi-xiong Zheng Jochen Ringe Zhong Liang Alexander Loch Li Chen Michael Sittinger | 2006 | Journal of Zhejiang University SCIENCE B2006，，10： | 1 |
| 6 | Mucosal flora in inflammatory bowel disease显示文摘 | Alexander Swidsinski Axel Ladhoff Annelie Pernthaler Sonja Swidsinski Vera Loening-Baucke Marianne Ortner Jutta Weber Uwe Hoffmann Stefan Schreiber Manfred Dietel Herbert Lochs | 2002 | Gastroenterology2002，，1： | 1 |
| 7 | Mucosal flora in inflammatory bowel disease显示文摘 | Alexander Swidsinski Axel Ladhoff Annelie Pernthaler Sonja Swidsinski Vera Loening-Baucke Marianne Ortner Jutta Weber Uwe Hoffmann Stefan Schreiber Manfred Dietel Herbert Lochs | 2002 | Gastroenterology2002，，1： | 1 |
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