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| 1 | Distribution of high-risk human papillomavirus genotype prevalence and attribution to cervical precancerous lesions in rural North China显示文摘Objective: Precise prevention is more desired for cervical cancer due to the huge population, high prevalence of human papillomavirus(HPV) infection in China and the vision of screen-and-treat strategies in low-and middleincome countries(LMICs). Considerations of combining type-specific prevalence and attribution proportion to high-grade cervical intraepithelial neoplasia are informative to more precise and effective region-specific cervical cancer prevention and control programs. The aim of the current study was to determine the genotype distribution of HPV and attribution to cervical precancerous lesions among women from rural areas in North China.Methods: A total of 9,526 women participated in the cervical cancer screening project in rural China. The samples of women who tested positive for HPV were retested with a polymerase chain reaction(PCR)-based HPV genotyping test. The attribution proportion of specific high-risk human papillomavirus(HR-HPV) types for different grades of cervical lesions was calculated by using the type contribution weighting method.Results: A total of 22.2%(2,112/9,526) of women were HR-HPV positive and HPV52(21.7%) was the most common HR-HPV genotype, followed by HPV58(18.2%), HPV53(18.2%) and HPV16(16.2%). The top three genotypes detected in HR-HPV-positive cervical intraepithelial neoplasia(CIN)1 were HPV16(36.7%), HPV58(20.4%), HPV56(15.3%). Among CIN2+, the most frequent genotypes were HPV16(75.6%), HPV52(17.8%),HPV58(16.7%). HPV16, 56, 58, 53, 52, 59, 68, and 18 combined were attributed to 84.17% of all CIN1 lesions,and HPV16, 58, and 52 combined were attributed to 86.98% of all CIN2+ lesions.Conclusions: The prevalence of HR-HPV infection among women from rural areas in North China was high and HPV16, HPV58, HPV52 had paramount attributable fraction in CIN2+. Type-specific HPV prevalence and attribution proportion to cervical precancerous lesions should be taken into consideration in the development of vaccines and strategy for screening in this population. | Shuang Zhao Xuelian Zhao Shangying Hu Jessica Lu Xianzhi Duan Xun Zhang Feng Chen Fanghui Zhao | 2019 | Chinese Journal of Cancer Research2019，31，4： | 30 |
| 2 | Genome-wide Scan for Seed Composition Provides Insights into Soybean Quality Improvement and the Impacts of Domestication and Breeding显示文摘优秀特点的复杂基因建筑学妨碍了努力在大豆修改种子营养素。染色体宽的协会研究为种子作文被进行,包括蛋白质,油,丰满的酸,和氨基酸,用有一个高密度的 SNP 数组的 313 多样的大豆 germplasm 就职 genotyped。87 个 chromosomal 区域的一个总数被识别与种子作文被联系,解释基因变化的 8%-89% 。已知的功能的候选人基因 GmSAT1, AK-HSDH, SACPD-C,和 FAD3A,和通常认为的 MtN21 nodulin, FATB,和 steroid-5 -- 涉及 N 2 固定,氨基酸生合成,和丰满的酸新陈代谢的 reductase 在主要效果的 loci 被发现。另外的 germplasm 就职的进一步的分析显示这些主要效果的 loci 受到了驯服或现代繁殖选择,并且突变而产生之遗传的变体和分布与地理区域相关。我们也表明与种子重量有关并且到全部的蛋白质的氨基酸集中有一个不同基因基础。这帮助揭开在种子之中的复杂关系的深入的基因机制加重。因此,我们的学习不仅为大豆营养素改进提供珍贵基因和标记,份量上和品质上,而且进在驯服期间优秀、引起的大豆的改变的提议卓见。 | Jiaoping Zhang Xianzhi Wang Yaming Lu Siddhi J. Bhusal Qijian Song Perry B. Cregan Yang Yen Michael Brown Guo-Liang Jiang | 2018 | Molecular Plant2018，11，3： | 12 |
| 3 | Effect of Soil Organic Matter on Adsorption and Insecticidal Activity of Toxins from Bacillus thuringiensis显示文摘With the large-scale cultivation of transgenic crops expressing Bacillus thuringiensis (Bt) insecticidal toxin in the world, the problem of environmental safety caused by these Bt crops has received extensive attention. The effects of soil organic matter (SOM) on the adsorption and insecticidal activity of Bt toxin in variable- and constant-charge soils (red and brown soils, respectively) were studied. Organic carbon in the soils was removed using hydrogen peroxide (H_2O_2). After H_2O_2 treatment, the SOM in the red and brown soils decreased by 71.26% and 82.82%, respectively. Mineral composition of the H_2O_2-treated soils showed no significant changes,but soil texture showed a slight change. After SOM removal, the cation exchange capacity (CEC) and pH decreased, while the specific surface area (SSA), point of zero charge (PZC), and zeta potential increased. The adsorption isotherm experiment showed that the Bt toxin adsorption on the natural and H_2O_2-treated soils fitted both the Langmuir model (R^2≥ 0.985 7) and the Freundlich model (R^2≥ 0.984 1), and the amount of toxin adsorbed on the H_2O_2-treated soils was higher than that on the natural soils. There was a high correlation between the maximum adsorption of Bt toxin and the PZC of soils (R^2= 0.935 7); thus, Bt toxin adsorption was not only influenced by SOM content, but also by soil texture, as well as the SSA, CEC, PZC, and zeta potential. The LC_(50) (lethal concentration required to kill 50% of the larvae) values for Bt toxin in the H_2O_2-treated soils were slightly lower than those in the natural soils, suggesting that the environmental risk from Bt toxin may increase if SOM decreases. As the measurement of insecticidal activity using insects is expensive and time consuming, a rapid and convenient in vitro method of enzyme-linked immunosorbent assays is recommended for evaluating Bt toxin degradation in soils in future studies. | ZHOU Xueyong YANG Zhe LIU Huifen LU Xianzhi HAO Jianchao | 2018 | Pedosphere2018，28，2： | 1 |
| 4 | An isoleucine-zipper motif enhances costimulation of human soluble trimeric GITR ligand显示文摘Glucocorticoid-induced tumor-necrosis factor receptor(GITR)and its ligand,GITRL,play significant roles in regulating immune responses.It is clear that human soluble GITRL(hsGITRL)transduces signal activity through multiple oligomerization states.To develop human soluble trimeric GITRL protein as a potential therapeutic target,we explored the link of the isoleucine-zipper(ILZ)motif to the N-terminus of the human soluble GITRL with two leucine sequences.hsGITRL,with the ILZ motif(ILZ-hsGITRL),was firstly expressed in Escherichia coli,which exhibited a predominant trimer when identified by Sephadex G-100 filtration and non-reducing SDS–polyacrylamide gel electrophoresis(SDS-PAGE).The significantly higher biological activity of the ILZ-hsGITRL compared with hsGITRL was confirmed by CD41 T proliferation,interferon-c(IFN-c)secretion and binding activity assay.To reveal and compare the underlying mechanisms,the level of extracellular signal-regulated kinase-1/2(ERK1/2)phosphorylation was examined,indicating that ILZ-hsGITRL induced more persistent and stronger ERK1/2 activation than hsGITRL.In conclusion,the incorporation of an ILZ motif could markedly improve the costimulation of hsGITRL. | Dawei Cui Shengjun Wang Yu Chen Jia Tong Jie Ma Li Tang Xianzhi Yang Ye Shi Jie Tian Liwei Lu Huaxi Xu | 2010 | Cellular & Molecular Immunology2010，7，4： | 0 |
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