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1篇 您的检索式:作者名="LUCIA F.LOPEZ"
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1An alternative to MINFLUX that enables nanometer resolution in a confocal microscope显示文摘Localization of single fluorescent emitters is key for physicochemical and biophysical measurements at the nanoscale and beyond ensemble averaging.Examples include single-molecule tracking and super-resolution imaging by single-molecule localization microscopy.Among the numerous localization methods available,MINFLUX outstands for achieving a~10-fold improvement in resolution over wide-field camera-based approaches,reaching the molecular scale at moderate photon counts.Widespread application of MINFLUX and related methods has been hindered by the technical complexity of the setups.Here,we present RASTMIN,a single-molecule localization method based on raster scanning a light pattern comprising a minimum of intensity.RASTMIN delivers~1-2nm localization precision with usual fluorophores and is easily implementable on a standard confocal microscope with few modifications.We demonstrate the performance of RASTMIN in localization of single molecules and super-resolution imaging of DNA origami structures.LUCIANO A.MASULLO ALAN M.SZALAI LUCIA F.LOPEZ MAURICIO PILO-PAIS GUILLERMO P.ACUNA Fernando D.Stefani 2022Light(Science & Applications)2022,11,9:2
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