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| 1 | Expression of matrix metalloproteinases 2 and 9 in human gastric cancer and superficial gastritis显示文摘AIM:To assess expression of matrix metalloproteinases 2(MMP2)and MMP9 in gastric cancer,superficial gastritis and normal mucosa,and to measure metalloproteinase activity.METHODS:MMP2 and MMP9 mRNA expression was determined by quantitative real-time polymerase chain reaction.Normalization was carried out using three different factors.Proteins were analyzed by quantitative gelatin zymography(qGZ).RESULTS:18S ribosomal RNA(18SRNA)was very highly expressed,while hypoxanthine ribosyltransferase-1(HPRT-1)was moderately expressed.MMP2 was highly expressed,while MMP9 was not detected or lowly expressed in normal tissues,moderately or highly expressed in gastritis and highly expressed in cancer.Relative expression of 18SRNA and HPRT-1 showed no significant differences.Significant differences in MMP2 and MMP9 were found between cancer and normal tissue,but not between gastritis and normal tissue.Absolute quantification of MMP9 echoed this pattern,but differential expression of MMP2 proved conflictive.Analysis by qGZ indicated significant differences between cancer and normal tissue in MMP-2,total MMP-9,250 and 110 kDa bands.CONCLUSION:MMP9 expression is enhanced in gastric cancer compared to normal mucosa;interpretation of differential expression of MMP2 is difficult to establish. | Clara Luz Sampieri Sol de la Pea Mariana Ochoa-Lara Roberto Zenteno-Cuevas Kenneth León-Córdoba | 2010 | World Journal of Gastroenterology2010，16，12： | 46 |
| 2 | Pancreatitis after endoscopic retrograde cholangiopancreatography:A narrative review显示文摘Acute post-endoscopic retrograde cholangiopancreatography pancreatitis(PEP)is a feared and potentially fatal complication that can be as high as up to 30%in high-risk patients.Pre-examination measures,during the examination and after the examination are the key to technical and clinical success with a decrease in adverse events.Several studies have debated on the subject,however,numerous topics remain controversial,such as the effectiveness of prophylactic medications and the amylase dosage time.This review was designed to provide an update on the current scientific evidence regarding PEP available in the literature. | Igor Braga Ribeiro Epifanio Silvino do Monte Junior Antonio Afonso Miranda Neto Igor Mendonça Proença Diogo Turiani Hourneaux de Moura Mauricio Kazuyoshi Minata Edson Ide Marcos Eduardo Lera dos Santos Gustavo de Oliveira Luz Sergio Eiji Matuguma Spencer Cheng Renato Baracat Eduardo Guimarães Hourneaux de Moura | 2021 | World Journal of Gastroenterology2021，27，20： | 5 |
| 3 | Arachidyl amido cholanoic acid improves liver glucose and lipid homeostasis in nonalcoholic steatohepatitis via AMPK and mTOR regulation显示文摘BACKGROUND Arachidyl amido cholanoic acid(Aramchol)is a potent downregulator of hepatic stearoyl-CoA desaturase 1(SCD1)protein expression that reduces liver triglycerides and fibrosis in animal models of steatohepatitis.In a phase IIb clinical trial in patients with nonalcoholic steatohepatitis(NASH),52 wk of treatment with Aramchol reduced blood levels of glycated hemoglobin A1c,an indicator of glycemic control.AIM To assess lipid and glucose metabolism in mouse hepatocytes and in a NASH mouse model[induced with a 0.1%methionine and choline deficient diet(0.1MCD)]after treatment with Aramchol.METHODS Isolated primary mouse hepatocytes were incubated with 20μmol/L Aramchol or vehicle for 48 h.Subsequently,analyses were performed including Western blot,proteomics by mass spectrometry,and fluxomic analysis with 13C-uniformly labeled glucose.For the in vivo part of the study,male C57BL/6J mice were randomly fed a control or 0.1MCD for 4 wk and received 1 or 5 mg/kg/d Aramchol or vehicle by intragastric gavage for the last 2 wk.Liver metabolomics were assessed using ultra-high-performance liquid chromatography-time of flight-MS for the determination of glucose metabolism-related metabolites.RESULTS Combination of proteomics and Western blot analyses showed increased AMPK activity while the activity of nutrient sensor mTORC1 was decreased by Aramchol in hepatocytes.This translated into changes in the content of their downstream targets including proteins involved in fatty acid(FA)synthesis and oxidation[PACCα/β(S79),SCD1,CPT1A/B,HADHA,and HADHB],oxidative phosphorylation(NDUFA9,NDUFB11,NDUFS1,NDUFV1,ETFDH,and UQCRC2),tricarboxylic acid(TCA)cycle(MDH2,SUCLA2,and SUCLG2),and ribosome(P-p70S6K[T389]and P-S6[S235/S236]).Flux experiments with 13Cuniformely labeled glucose showed that TCA cycle cataplerosis was reduced by Aramchol in hepatocytes,as indicated by the increase in the number of rounds that malate remained in the TCA cycle.Finally,liver metabolomic analysis showed that glucose homeostasis was improved by Aramchol in 0.1MCD fed mice in a dose-dependent manner,showing normalization of glucose,G6P,F6P,UDP-glucose,and Rbl5P/Xyl5P.CONCLUSION Aramchol exerts its effect on glucose and lipid metabolism in NASH through activation of AMPK and inhibition of mTORC1,which in turn activate FAβ-oxidation and oxidative phosphorylation. | David Fernández-Ramos Fernando Lopitz-Otsoa Laura Delacruz-Villar Jon Bilbao Martina Pagano Laura Mosca Maider Bizkarguenaga Marina Serrano-Macia Mikel Azkargorta Marta Iruarrizaga-Lejarreta Jesús Sot Darya Tsvirkun Sebastiaan Martijn van Liempd Felix M Goni Cristina Alonso María Luz Martínez-Chantar Felix Elortza Liat Hayardeny Shelly C Lu JoséM Mato | 2020 | World Journal of Gastroenterology2020，26，34： | 5 |
| 4 | Mesenchymal stem cell therapy in retinal and optic nerve diseases: An update of clinical trials显示文摘Retinal and optic nerve diseases are degenerative ocular pathologies which lead to irreversible visual loss. Since the advanced therapies availability, cell-based therapies offer a new all-encompassing approach. Advances in the knowledge of neuroprotection, immunomodulation and regenerative properties of mesenchymal stem cells(MSCs) have been obtained by several preclinical studies of various neurodegenerative diseases. It has provided the opportunity to perform the translation of this knowledge to prospective treatment approaches for clinical practice. Since 2008, several first steps projecting new treatment approaches, have been taken regarding the use of cell therapy in patients with neurodegenerative pathologies of optic nerve and retina. Most of the clinical trials using MSCs are in Ⅰ/Ⅱ phase, recruiting patients or ongoing, and they have as main objective the safety assessment of MSCs using various routes of administration. However, it is important to recognize that, there is still a long way to go to reach clinical trials phase Ⅲ-Ⅳ. Hence, it is necessary to continue preclinical and clinical studies to improve this new therapeutic tool. This paper reviews the latest progress of MSCs in human clinical trials for retinal and optic nerve diseases. | Sonia Labrador-Velandia María Luz Alonso-Alonso Sara Alvarez-Sanchez Jorge González-Zamora Irene Carretero-Barrio José Carlos Pastor Iván Fernandez-Bueno Girish Kumar Srivastava | 2016 | World Journal of Stem Cells2016，8，11： | 5 |
| 5 | S-adenosyl-L-methionine modifies antioxidant-enzymes,glutathione-biosynthesis and methionine adenosyltransferases-1/2 in hepatitis C virus-expressing cells显示文摘AIM: To elucidate the mechanism(s) by which S-adenosyl-L-methionine(SAM) decreases hepatitis C virus(HCV) expression.METHODS: We examined the effects of SAM on viral expression using an HCV subgenomic replicon cell culture system. Huh7 HCV-replicon cells were treated with 1 mmol/L SAM for different times(24-72 h), then total RNA and proteins were isolated. c DNA was synthesized and real time-PCR was achieved to quantify HCV-RNA, superoxide dismutase 1 and 2(SOD-1, SOD-2) catalase, thioredoxin 1, methionine adenosyltransferase 1A and 2A(MAT1A, MAT2A) expression, and GAPDH and RPS18 as endogenous genes. Expression of cellular and viral protein was evaluated by western-blot analysis using antibodies vs HCV-NS5 A, SOD-1, SOD-2, catalase, thioredoxin-1, MAT1 A, MAT2 A, GAPDH and actin. Total glutathione levels were measured at different times by Ellman's recycling method(0-24 h). Reactive oxidative species(ROS) levels were quantified by the dichlorofluorescein assay(0-48 h); Pyrrolidin dithiocarbamate(PDTC) was tested as an antioxidant control and H2O2 as a positive oxidant agent.RESULTS: SAM exposition decreased HCV-RNA levels 50%-70% compared to non-treated controls(24-72 h). SAM induced a synergic antiviral effect with standard IFN treatment but it was independent of IFN signaling. In addition, 1 mmol/L SAM exposition did not modify viral RNA stability, but it needs cellular translation machinery in order to decrease HCV expression. Total glutathione levels increased upon SAM treatment in HCV-replicon cells. Transcriptional antioxidant enzyme expression(SOD-1, SOD-2 and thioredoxin-1) was increased at different times but interestingly, there was no significant change in ROS levels upon SAM treatment, contrary to what was detected with PDTC treatment, where an average 40% reduction was observed in exposed cells. There was a turnover from MAT1A/MAT2 A, since MAT1 A expression was increased(2.5 fold-times at 48 h) and MAT2 A was diminished(from 24 h) upon SAM treatment at both the transcriptional and translational level. CONCLUSION: A likely mechanism(s) by which SAM diminish HCV expression could involve modulating antioxidant enzymes, restoring biosynthesis of glutathione and switching MAT1/MAT2 turnover in HCV expressing cells. | Sonia Amelia Lozano-Sepulveda Eduardo Bautista-Osorio Jose Angel Merino-Mascorro Marta Varela-Rey Linda Elsa Munoz-Espinosa Paula Cordero-Perez María Luz Martinez-Chantar Ana Maria Rivas-Estilla | 2016 | World Journal of Gastroenterology2016，22，14： | 2 |
| 6 | Trends in overall opportunistic illnesses, Pneumocystis carinii pneumonia, cerebral toxoplasmosis and Mycobacterium avium complex incidence rates over the 30 years of the HIV epidemic: a systematic review显示文摘 | Lara Coelho Valdiléa Gon?alves Veloso Beatriz Grinsztejn Paula Mendes Luz | 2013 | Brazilian Journal of Infectious Diseases2013，，： | 2 |
| 7 | A survey of indigenous microbial hydrocarbon degradation genes in soils from Antarctica and Brazil显示文摘 | Luz A P Pellizari V H Whyte L G | 2004 | Can J Microbiol2004，50，： | 1 |
| 8 | Multidisciplinary design optimization of an automotive magnetorheological brake design 显示文摘 | Park E Luz L Suleman A | 2008 | Computers gy- Structures2008，86，35： | 1 |
| 9 | Extraction of Egg-Yolk Lecithin显示文摘 | Luz E Palacios a Tong Wang | 2005 | JAOCS2005，82，8： | 1 |
| 10 | Lethal skeletal dysplasia from targeted disruption of the parathyroid hormone - re- lated peptide gene显示文摘 | KARAPLIS A C LUZ A GLOWACKI J | 1994 | Genes Dev1994，8，3： | 1 |
| 11 | Oxygen isotope variations in phosphate of deer bones显示文摘 | Luz B Cormie A B Schwarcz H P | | 0，，06： | 1 |
| 12 | Pathologic complete response after neoadjuvant treatment for rectal cancer decreases distant recurrence and could eradicate local recurrence 显示文摘 | de Campos-Lobato LF Stocchi L da Luz Moreira A | 2011 | Ann Surg Oncol2011，18，： | 1 |
| 13 | Physicochemical, morphological, and pasting properties of nixtamalized flours from quality protein maize and its particle distribution显示文摘 | María de los ángeles Cornejo-Villegas Elsa Gutiérrez-Cortez Isela Rojas-Molina Alicia Del Real-López María de la Luz Zambrano-Zaragoza Viridiana Martínez-Vega Mario E. Rodríguez-García | 2013 | LWT - Food Science and Technology2013，，1： | 1 |
| 14 | Infliximab in ulcerative colitis is associated with an increased risk of postoperative complications after restorative proctocolectomy显示文摘 | Mor IJ Vogel JD Da Luz Moreira A | 2008 | Dis Colon Rectum2008，51，8： | 1 |
| 15 | Seroprevalence and national distribution of human toxoplasmosis in Mexico: analysis of the 2000 and 2006 National Health Surveys显示文摘 | Heriberto Caballero-Ortega Felipe Javier Uribe-Salas Carlos J. Conde-Glez Carlos Cedillo-Pelaez José Antonio Vargas-Villavicencio Héctor Luna-Pastén Irma Ca?edo-Solares Luz Belinda Ortiz-Alegría Dolores Correa | 2012 | Transactions of the Royal Society of Tropical Medicine and Hygiene2012，，11： | 1 |
| 16 | Randomized trial comparing overnight preoperative fasting period vs oral administration of apple juice at 06:00 -06:30 AM in pediatric orthopedic surgical patients 显示文摘 | Castillo ZC Castillo P Luz A | 2005 | Paediatric Anaesthesia2005，15，8： | 1 |
| 17 | Pathologic complete response after neoadjuvant treatment for rectal cancer decreases distant recurrence and could eradicate local recurrence显示文摘 | de Campos-Lobato LF Stocchi L da Luz Moreira A | 2011 | Ann Surg Oncol2011，18，6： | 1 |
| 18 | Myocardial bridging: retrospective analysis and scintigraphic testing of patients with no anglographically significant atherosclerotic coronary disease显示文摘 | Luz A Selveira J Palma P | 2006 | Rev Port Cardiol2006，25，4： | 1 |
| 19 | TEG(R) and ROTEM(R) in trauma: similar test but different results?显示文摘 | Sankaranlmtty A Nascimanto B Teodoro da Luz L | 2012 | World J Emerg Surg2012，71，： | 1 |
| 20 | Effect of foliar and substrate fertilization on lady palm seedling growth and development显示文摘 | Da Luz P B Patrcia Duarte de 0liveira Paiva P D Tavares A R | | 0，，： | 1 |
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