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| 1 | Differences in lead isotopic fingerprints between blood, hair and organs in lead-poisoned rats显示文摘Lead isotopic ratios of biosamples such as blood, hair and teeth are used as 'fingerprints' to trace and identify sources of lead pollution in the environment. However, the reliability of this technique has not been confirmed. In previous studies, we investigated the differences in lead isotopic ratios between blood, hair, and organs from the same human body. To explore the relation-ship between isotopic ratios in environmental lead and biosamples, and to investigate the influence of exposure dose, rats were exposed to 0, 30, 60, and 120 mg/kg body weight of lead acetate every day for 4 weeks by intragastric administration. Lead iso- topic ratios of blood, hair, liver, kidney and femur in rats, as well as that of the test substance lead acetate, were measured using inductively coupled plasma mass spectrometry. There were significant differences (P<0.05) in lead isotopic fingerprints between blood, hair, and tissues. Lead fingerprints of blood were influenced by lead exposure dose, while those of other tissues and organs were generally not. Its reliability and availability indicated that blood, but not hair, was thus a suitable biomarker for environmental lead exposure. The results of this study provide the scientific rationale for the use of lead isotopic ratios for the identification of human lead pollution sources. | ZENG Jing YAN LaiLai OUYANG Li WU Jing WANG JingYu | 2012 | Chinese Science Bulletin2012，57，7： | 1 |
| 2 | Associations of particulate matter with dementia and mild cognitive impairment in China: A multicenter cross-sectional study显示文摘Ambient air pollution has been shown to be associated with the pathogenesis of dementia and mild cognitive impairment(MCI).However,few studies have examined these associations in well-characterized populations with low residential mobility,similar living habits,and a standardized assessment of both air pollution exposure and clinical outcome.This study examined the associations of long-term exposure to particulate matter(PM)air pollution with dementia and MCI,using data from the Chinese Veteran Clinical Research Platform.The cognitive function of elderly veterans from 277 communities in 18 Chinese cities was examined.Participants’daily exposures to aerodynamic diameters%2.5 mm(PM2.5)and%10 mm(PM10)during the 3 years prior to the survey were estimated using a satellite-based prediction.The adjusted odds ratios(ORs)and 95%confidence intervals of MCI associated with each 10 mg/m^(3) increase in PM2.5 and PM10 were 1.52(1.39,1.67)and 1.04(1.00,1.08),and those of dementia associated with PM2.5 and PM10 were 1.27(1.11,1.46)and 1.13(1.05,1.21),respectively.This demonstrates that long-term exposure to PM2.5 and PM10 can increase the prevalence of dementia/MCI among veterans in China.Higher ORs were observed for those with%9 years of educational attainment,those who actively attended physical activities,those who never smoked,former drinkers,and those who did not suffer from cerebral infarction.Improvement of ambient air quality,especially decreasing levels of PM2.5,may help to decrease the risk of dementia/MCI.Given the statistically significant association between PM and cognitive impairment demonstrated here,future studies should focus on examining the causal effect of PM pollution on dementia and MCI. | Jiping Tan Nan Li Xiaoxiao Wang Gongbo Chen Lailai Yan Luning Wang Yiming Zhao Shanshan Li Yuming Guo | 2021 | The Innovation2021，2，3： | 1 |
| 3 | Protective Effect and Molecular Mechanism of Shui People's Classic Prescription Jipei Dilong Ointment on Osteoarthritis in Rats显示文摘[Objectives]To explore the protective effect and molecular mechanism of Shui People s Classic Prescription Jipei Dilong Ointment on knee osteoarthritis in rats.[Methods]72 SPF male SD rats were divided into control group,model group,Jipei Dilong Ointment high,medium and low dose groups,and positive drug Diclofenac group,with 12 rats in each group.Except the control group,all other groups were injected with 0.2 mL of 2%papain and 0.3%L-cysteine mixture into knee joint cavity to establish osteoarthritis model,while the control group was injected with the same amount of normal saline.The Lequesne MG score was used to determine the success of the model.After successful modeling,external administration was given for 4 weeks.The histopathological changes in articular cartilage and synovium were observed by HE staining;the levels of TNF-α,IL-1βand COX-2 in serum were detected by ELISA,and the relative expression of MMP-9 and TIMP-1 mRNA in cartilage was detected by qRT-PCR;the relative expression of MMP-9,TIMP-1,TLR4,MyD88 and NF-κB in rat cartilage was detected by Western-blot.[Results]Compared with the model group,Jipei Dilong Ointment could significantly reduce the Lequesne MG score and Mankin s score of arthritic rats(P<0.01);significantly improve the pathological changes in articular cartilage and synovium,reduce tissue edema,necrosis,inflammatory cell infiltration and fibrous tissue proliferation;reduce the expression of MMP-9 mRNA(P<0.01)in different degrees and increase the expression of TIMP-1 mRNA(P<0.01);reduce the relative expression of MMP-9,TLR4,MyD88 and NF-κB protein in different degrees(P<0.01),and significantly increase the relative expression of TIMP-1 protein in cartilage(P<0.01).[Conclusions]Jipei Dilong Ointment can improve the joint injury of osteoarthritis rats,and the mechanism may be related to the regulation of TLR4/MyD88/NF-κB signaling pathway and the ratio of MMP-9/TIMP-1. | Lailai LI Baoying HUA Shiyun YE Yihui CHAI Haotian WANG Jinghua RUAN Xiang PU Liyan ZHANG Sibu MA | 2023 | Medicinal Plant2023，14，5： | 0 |
| 4 | Study on the Molecular Mechanism of Danggui Buxue Decoction in Intervention of Perimenopausal Syndrome Based on Network Pharmacology and Molecular Docking显示文摘[Objectives]This study was conducted to explore the intervention mechanism of Danggui Buxue Decoction in perimenopausal syndrome based on network pharmacology and molecular docking.[Methods]The chemical components and targets of Danggui Buxue Decoction were acquired through the TCMSP database,and the main targets of perimenopausal syndrome were obtained through the GeneCards database.The component targets and disease targets were intersected,and combining with active components and Chinese herbs in the decoction,a traditional Chinese medicine-component-target network was constructed using Cytoscape 3.7.1 software.The STRING platform was employed for protein-protein interaction analysis.The DAVID analysis platform was used to conduct target GO and KEGG enrichment analysis,so as to predict the action mechanism Danggui Buxue Decoction.Finally,an active component-disease target-signal pathway network diagram was constructed.[Results]Twenty two components in Danggui Buxue Decoction related to perimenopausal syndrome and 120 corresponding targets were obtained,including active components such as 1,7-dihydroxy-3,9-dimquercetin and kaempferol,and key targets such as TNF,ESR1 and PPARG.The results of GO analysis and KEEG analysis indicated that Danggui Buxue Decoction might regulate the transcription of RNA polymerase II promoter,DNA templating,gene expression,signal transduction,hypoxia response and other biological processes by regulating multiple signal pathways such as chemical carcinogenesis-receptor activation,cancer pathways,lipid and atherosclerosis,tryptophan metabolism,malaria,steroid hormone biosynthesis and chemical carcinogenesis-DNA adduct.[Conclusions]Danggui Buxue Decoction intervenes in perimenopausal syndrome through multiple components,targets and pathways,providing a basis for elucidating the intervention mechanism of Danggui Buxue Decoction and expanding its clinical application. | Na LI Shunhuan CHEN Xiang PU Yihui CHAI Yuqi YANG Lailai LI | 2023 | Agricultural Biotechnology2023，12，6： | 0 |
| 5 | Therapeutic Effect and Mechanism of Jipei Dilong Ointment on Acute Soft Tissue Injury in Rats显示文摘[Objectives]This study was conducted to observe the therapeutic effect of Jipei Dilong Ointment on rats with acute soft tissue injury caused by heavy objects and to explore its action mechanism.[Methods]Thirty six rats were randomly divided into six groups(control group,model group,high-dose Jipei Dilong Ointment group(JP-H),medium-dose Jipei Dilong Ointment group(JP-M),low-dose Jipei Dilong Ointment group(JP-L)and diclofenac group).Except for the Control group,other groups were subjected to modeling of acute soft tissue injury by the weight impact method.All administration was performed once a day for nine consecutive days.The local appearance score and activity disorder score were determined after soft tissue injury in rats.HE staining was used to detect the pathological changes of injured soft tissues in rats.RT-PCR was used to detect the relative mRNA expressions of Bax,Bcl-2,MMP-9 and TIMP-1 in injured soft tissues of rats.Western Blot was used to detect the protein expressions of MMP-9,TIMP-1,TLR4,MyD88 and NF-κB p65 in injured soft tissues of rats.Results were statistically analyzed.[Results]Compared with the model group,Jipei Dilong Ointment could significantly improve the appearance symptoms such as swelling and ecchymosis in the injured area and the movement function of the affected limb(P<0.05).It could also improve the infiltration of inflammatory cells and widening of the intermuscular space caused by injury.Among them,the JP-H group and the diclofenac group had more significant curative effects.After 9 d of administration,each administration group could significantly up-regulate the ratio of Bcl-2/Bax mRNA expression level(P<0.05 or P<0.01),and the ratio of MMP-9/TIMP-1 mRNA expression level showed a downward trend(P>0.05).The expression level of NF-κB p65 protein in each administration group was significantly decreased(P<0.01).The protein expression levels of TLR4 and MyD88 and the ratio of MMP-9/TIMP-1 protein expression level in each administration group decreased to varying degrees.Among them,the JP-H group and diclofenac group significantly decreased(P<0.05).[Conclusions]Jipei Dilong Ointment has the functions of relieving pain,swelling and inflammation.It could improve the local appearance,functional activity and tissue morphology of affected limbs in rats,and has a therapeutic effect on acute soft tissue injury in rats.Its mechanism of action might be related to the inhibition of TLR4/MyD88/NF-κB p65 signaling pathway and the regulation of Bcl-2/Bax and MMP-9/TIMP-1 balance. | Yihui CHAI Haotian WANG Shiyun YE Lailai LI Baoying HUA Jinghua RUAN Xiang PU Liyan ZHANG Sibu MA | 2023 | Agricultural Biotechnology2023，12，5： | 0 |
| 6 | Study on the Mechanism of Pseudostellariae Radix in Regulating Angiogenesis Based on Network Pharmacology and a Dual-screening System显示文摘[Objectives]This study was conducted to clarify the action mechanism of Pseudostellariae Radix in regulating angiogenesis by using network pharmacology and a dual-screening system,and to provide a basis for its clinical treatment of cardiovascular diseases.[Methods]The TCMSP database was used for preliminary screening to obtain the active compounds of Pseudostellariae Radix and the protein targets of its action.GeneCards and OMIM databases were used to search for targets related to angiogenesis.Cytoscape 3.9.1 was used to construct a drug-target network and protein interaction network of Pseudostellariae Radix in angiogenesis.The GO enrichment analysis and KEGG pathway analysis of the targets of Pseudostellariae Radix in angiogenesis were carried out on Metascape platform.The effects of the screened active compounds were verified using a dual-screening system.[Results]Six active components of Pseudostellariae Radix,luteolin,acetin,beta-sitosterol,linarin,schottenol and 1-monolinolein,were screened by TCMSP database;and the six active components were predicted with 78 common target proteins related to angiogenesis,of which 19 were core targets.Pseudostellariae Radix mainly intervened in angiogenesis through domain specific binding,ubiquitin-like protein ligase binding,kinase binding and other molecular functions to regulate biological processes such as membrane microdomain,plasma membrane raft and caveola.The results of KEGG enrichment indicated that pathways in cancer,lipid and atherosclerosis,hepatitis B,apoptosis,toxoplasmosis and other key pathways might be the mechanism for the intervention of angiogenesis.The results of the dual-screening system showed that luteolin,acacetin,beta-sitosterol and linarin protected HUVECs and promoted zebrafish angiogenesis.[Conclusions]This study preliminarily demonstrated that luteolin,acacetin,beta-sitosterol and linarin could intervene in angiogenesis through multiple targets and multiple pathways,providing ideas and a scientific basis for the treatment of cardiovascular diseases. | Na LI Yihui CHAI Yuqi YANG Xiang PU Guo FENG Sibu MA Lailai LI | 2023 | Agricultural Biotechnology2023，12，5： | 0 |
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