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您的检索式：作者名="Laiqing Song"
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| 1 | Functional Characterization of the Apple RING E3 Ligase MdMIEL1 in Transgenic Arabidopsis显示文摘E3 ubiquitin ligases are involved in various physiological processes,and they play pivotal roles in growth and development.In this study,we identified a previously unknown gene in the apple fruit(Malus×domestica)and named it MdMIEL1.The MdMIEL1 gene encoded a protein that contained a zinc-finger domain at its N-terminus and a RING-finger motif at its C-terminus.To investigate MdMIEL1 functions,we generated transgenic Arabidopsis lines expressing the MdMIEL1 gene under the control of the Cauliflower mosaic virus 35S promoter.Interestingly,ectopic expression of MdMIEL1 in Arabidopsis produced multiple phenotypes,including early germination,early flowering and a lateral root number increase relative to wild-type plants.Further analysis indicated that MdMIEL1 regulated lateral root initiation by increasing auxin accumulation in the roots.In a word,these results suggest that,MdMIEL1 as a novel RING-finger ubiquitin ligase influences plant growth and development,and highlight that MdMIEL1 regulates lateral root growth. | AN Jianping LIU Xin SONG Laiqing YOU Chunxiang WANG Xiaofei HAO Yujin | 2017 | Horticultural Plant Journal2017，3，2： | 3 |
| 2 | Functional identification of MdSMXL8.2,the homologous gene of strigolactones pathway repressor protein gene in Malus×domestica显示文摘A homologous gene of strigolactones repressor protein gene SMXL7/D53,MdSMXL8.2(GenBank accession No.:MD07G1222400),was cloned from‘Royal Gala’apple(Malus×domestica Borkh.)in this study.The sequence analysis revealed that the length of this gene was 3243 bp,which encoded 1080 amino acids,and had a protein molecular mass of∼110 kD.The phylogenetic tree analysis indicated that the MdSMXL8.2 exhibited the highest sequence similarity with Arabidopsis AtSMXL7.The protein conserved domain analysis revealed that the MdSMXL8.2 contained two ClpA domains.The prediction of the secondary and tertiary structures of the MdSMXL8.2 indicated that it contained 34.54%αhelix,3.43%β-sheet,and 11.76%extended chain.The in-silico analysis suggested that the promoter sequence of MdSMXL8.2 contained several typical cisacting elements,including abscisic acid(ABA),gibberellin(GA),ethylene,auxin,jasmonic acid(JA),salicylic acid(SA),drought,and heat stressresponsive elements.Quantitative real-time(qRT)-PCR analyses revealed that MdSMXL8.2 was expressed in different apple tissues,with the highest transcript level found in the stem.The expression of MdSMXL8.2 was significantly induced by exogenous ABA,PEG and mannitol,while exogenous NaCl significantly inhibited MdSMXL8.2 expression.The growing status of MdSMXL8.2-overexpressed Orin apple callus was worse than the wild type(WT)after NaCl treatment and had a higher malondialdehyde(MDA)content and relative conductance(REC).Additionally,MdSMXL8.2-overexpressed Arabidopsis exhibited shorter root length and a reduction in fresh weight under salt stress,indicating that MdSMXL8.2 negatively regulated salt tolerance in apples. | Weijian Sun Xinglong Ji Laiqing Song Xiaofei Wang Chunxiang You Yujin Hao | 2021 | Horticultural Plant Journal2021，7，4： | 1 |
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