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| 1 | Structural and molecular features of intestinal strictures in rats with Crohn's-like disease显示文摘AIM: To develop a new rat model we wanted to gain a better understanding of stricture formation in Crohn's disease(CD).METHODS: Chronic colitis was induced locally by the administration of 2,4,6-trinitrobenzenesulfonic acid(TNBS). The relapsing inflammation characteristic to CD was mimicked by repeated TNBS treatments. Animals were randomly divided into control, once, twice and three times TNBS-treated groups. Control animals received an enema of saline. Tissue samples were taken from the strictured colonic segments and also adjacent proximally and distally to its 60, 90 or 120 d after the last TNBS or saline administrations. The frequency and macroscopic extent of the strictures were measured on digital photographs. The structural features of strictured gut wall were studied by light- and electron microscopy. Inflammation related alterations in TGF-beta 2 and 3, matrix metalloproteinases 9(MMP9) and TIMP1 m RNA and protein expression were determined by quantitative real-time PCR and western blot analysis. The quantitative distribution of caspase 9 was determined by post-embedding immunohistochemistry.RESULTS: Intestinal strictures first appeared 60 d after TNBS treatments and the frequency of them increased up to day 120. From day 90 an intact lamina epithelialis, reversible thickening of lamina muscularis mucosae and irreversible thickening of the muscularis externa were demonstrated in the strictured colonic segments. Nevertheless the morphological signs of apoptosis were frequently seen and excess extracellular matrix deposition was recorded between smooth muscle cells(SMCs). Enhanced caspase 9 expression on day 90 in the SMCs and on day 120 also in myenteric neurons indicated the induction of apoptosis. The m RNA expression profile of TGF-betas after repeated TNBS doses was characteristic to CD, TGF-beta 2, but not TGF-beta 3 was up-regulated. Overexpression of MMP9 and down-regulation of TIMP1 were demonstrated. The progressive increase in the amount of MMP9 protein in the strictures was also obvious between days 90 and 120 but TIMP1 protein was practically undetectable at this time.CONCLUSION: These findings indicate that aligned structural and molecular changes in the gut wall rather than neuronal cell death play the primary role in stricture formation. | Petra Talapka Anikó Berkó Lajos István Nagy Lalitha Chandrakumar Mária Bagyánszki László Géza Puskás éva Fekete Nikolett Bódi | 2016 | World Journal of Gastroenterology2016，22，22： | 3 |
| 2 | Brain abscess and other protean manifestation of actinomycosis显示文摘 | Koshi G Lalitha MK Samraj T | 1981 | Am J Trop Med Hyg1981，，： | 1 |
| 3 | 显示文摘 | Vishu Kumar A B VAradaraj M C Lalitha R G | 2004 | Biochimicaer Biophysica Acta2004，1670，2： | 1 |
| 4 | Stability of parametric quasivariational inequality of the minty type显示文摘 | Lalitha C S Bhatia G | 2011 | Journal of Optimization Theory and Applications2011，148，： | 1 |
| 5 | Low molecular weight chitosans: Preparation with the aid of papain and characterization显示文摘 | VISHU KUMAR A B VARADARAJ M C LALITHA R G | 2004 | Bioehimiea et Biophysiea Aeta ( BBA ) : General Subjects2004，1670，2： | 1 |
| 6 | Ellagic acid encapsulated chitosan nanoparticles as anti-hemorrhagic agent显示文摘 | Lalitha G Lakshmi N R Swaminathan S | 2014 | Carbohydrate Polymers2014，111，10： | 1 |
| 7 | Higly stabilized and disperd CuO/TiO2: A promising visible senstive photocatalyst for continuous production ofhydrogden from glycerol: water mixtures 显示文摘 | Lalitha K Sadanandam G Kumari V D | 2010 | J Phys Chem C2010，114，22： | 1 |
| 8 | Making an Indian traditional rice variety Mahsuri,bacterial blight resistant using marker-assisted selection显示文摘 | Lalitha D G Pranitha K Vinay S | 2013 | Journal of Crop Science and Biotechnology2013，16，2： | 1 |
| 9 | Chitosanolysis by a pectinase isozyme of Aspergillus niger-A non-specific activity显示文摘 | KTTTUR F S KUMAR A LALITHA R G | 2003 | Carbohydr Polym2003，53，： | 1 |
| 10 | Protective effect of neferine against isoproterenol-induced cardiac toxicity 显示文摘 | Lalitha G Poornima P Archanah A | 2013 | Cardiovasc Toxicol2013，13，2： | 1 |
| 11 | Utilization of meat industry by products: Protein hydrolysate from sheep visceral mass 显示文摘 | Bhaskar N Modi V K Govindaraju K Radha C Lalitha R G | 2007 | Bioresource Technology2007，98，： | 1 |
| 12 | Low molecular weight chitosans: preparation with the aid of papain and characterization 显示文摘 | Kumar A B Varadaraj M C Lalitha R G | 2004 | Biochimica et Biophysica Acta2004，1670，2： | 1 |
| 13 | Low molecular weight chitosans: preparation with the aidof papain and characterization显示文摘 | KUMAR AB V VARADARAJ M C LALITHA R G etal | 2004 | Biochimica of BiophysicaActa2004，，1670： | 1 |
| 14 | Angiotensin I-converting enzyme inhibitory peptide derived from glycinin, the 11S globulin of soybean (Glycine max)显示文摘 | Mallikarjun K G Lalitha R G Appurao A G | 2006 | Journal of Agricultural and Food Chemistry2006，54，13： | 1 |
| 15 | Alginate beads of Captopril using galactomannan containing Senna tora gum, guar gum and locust bean gum显示文摘 | Pawar H A Lalitha K G Ruckmani K | 2015 | International Journal of BiologicalMacromolecules2015，76，： | 1 |
| 16 | Improved audio water- marking using DWT-SVD显示文摘 | LALITHA N V SURESH G Dr SAILAJA Y | 2011 | International Journal of Scientific & Engineering Research2011，2，6： | 1 |
| 17 | Making an Indian trandi- tional rice variety Mahuri, bacterial blight resistant using mark as- sisted selection显示文摘 | Lalitha D G Pranitha K Vinay S | 2013 | JCSB2013，16，2： | 1 |
| 18 | Brain abscess and other protean manifestation of actinomycosis显示文摘 | Koshi G Lalitha MK Samraj T Mathai KV | 1981 | Am J Trop Med Hyg1981，30，： | 1 |
| 19 | Low molecular weight chitosans: preparation with the aid of papain and characterization显示文摘 | Kumar A V Varadaraj M C Lalitha R G | 2004 | BBA2004，1670，： | 1 |
| 20 | Optimality criteria in set-valued optimization显示文摘 | Lalitha C S Dutta J Govll M G | 2003 | J Anst Math SOc2003，75，： | 1 |
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