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| 1 | Inhibition of SARS-CoV-2 (previously 2019-nCoV) infection by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion显示文摘The recent outbreak of coronavirus disease(COVID-19)caused by SARS-CoV-2 infection in Wuhan,China has posed a serious threat to global public health.To develop specific anti-coronavirus therapeutics and prophylactics,the molecular mechanism that underlies viral infection must first be defined.Therefore,we herein established a SARS-CoV-2 spike(S)protein-mediated cell-cell fusion assay and found that SARS-CoV-2 showed a superior plasma membrane fusion capacity compared to that of SARS-CoV.We solved the X-ray crystal structure of six-helical bundle(6-HB)core of the HR1 and HR2 domains in the SARS-CoV-2 S protein S2 subunit revealing that several mutated amino acid residues in the HR1 domain may be associated with enhanced interactions with the HR2 domain.We previously developed a pan-coronavirus fusion inhibitor,EK1,which targeted the HR!domain and could inhibit infection by divergent human coronaviruses tested,including SARS-CoV and MERS-CoV.Here we generated a series of lipopeptides derived from EK1 and found that EK1C4 was the most potent fusion inhibitor against SARS-CoV-2 S protein-mediated membrane fusion and pseudovirus infection with IC50s of 1.3 and 15.8 nM,about 241-and 149-fold more potent than the original EK1 peptide,respectively.EK1C4 was also highly effective against membrane fusion and infection of other human coronavirus pseudoviruses tested,including SARS-CoV and MERS-CoV,as well as SARSr-CoVs,and potently inhibited the replication of 5 live human coronaviruses examined,including SARS-CoV-2.Intranasal application of EK1C4 before or after challenge with HCoV-OC43 protected mice from infection,suggesting that EK1C4 could be used for prevention and treatment of infection by the currently circulating SARS-CoV-2 and other emerging SARSr-CoVs. | Shuai Xia Meiqin Liu Chao Wang Wei Xu Qiaoshuai Lan Siliang Feng Feifei Qi Linlin Bao Lanying Du Shuwen Liu Chuan Qin Fei Sun Zhengli Shi Yun Zhu Shibo Jiang Lu Lu | 2020 | Cell Research2020，30，4： | 81 |
| 2 | The Genome Sequence Archive Family: Toward Explosive Data Growth and Diverse Data Types显示文摘The Genome Sequence Archive(GSA)is a data repository for archiving raw sequence data,which provides data storage and sharing services for worldwide scientific communities.Considering explosive data growth with diverse data types,here we present the GSA family by expanding into a set of resources for raw data archive with different purposes,namely,GSA(http://gffzz77e3413bc06540edskv6nv5f0vqob6kpf.ffgz.tsg.suse.edu.cn/gsa/),GSA for Human(GSA-Human,http://gffzz77e3413bc06540edskv6nv5f0vqob6kpf.ffgz.tsg.suse.edu.cn/gsa-human/),and Open Archive for Miscellaneous Data(OMIX,http://gffzz77e3413bc06540edskv6nv5f0vqob6kpf.ffgz.tsg.suse.edu.cn/omix/).Compared with the 2017 version,GSA has been significantly updated in data model,online functionalities,and web interfaces.GSA-Human,as a new partner of GSA,is a data repository specialized in human genetics-related data with controlled access and security.OMIX,as a critical complement to the two resources mentioned above,is an open archive for miscellaneous data.Together,all these resources form a family of resources dedicated to archiving explosive data with diverse types,accepting data submissions from all over the world,and providing free open access to all publicly available data in support of worldwide research activities. | Tingting Chen Xu Chen Sisi Zhang Junwei Zhu Bixia Tang Anke Wang Lili Dong Zhewen Zhang Caixia Yu Yanling Sun Lianjiang Chi Huanxin Chen Shuang Zhai Yubin Sun Li Lan Xin Zhang Jingfa Xiao Yiming Bao Yanqing Wang Zhang Zhang Wenming Zhao | 2021 | Genomics, Proteomics & Bioinformatics2021，19，4： | 38 |
| 3 | Does Helicobacter pylori infection play a role in iron deficiency anemia? A meta-analysis显示文摘AIM:To perform a meta-analysis of observational studies and randomized controlled trials(RCTs)on the association between Helicobacter pylori(H.pylori)and iron deficiency anemia(IDA).METHODS:A defined search strategy was used to search Medline,Embase,the Cochrane Library,Clinical Trials,Cochrane Central Register of Controlled Trials,Premedline and Healthstar.Odds ratio(OR)was used to evaluate observational epidemiology studies,and weighted mean difference(WMD)was used to demonstrate the difference between control and intervention groups.RESULTS:Fifteen observational studies and 5 RCTs were identified and used for calculation.The pooled OR for observational studies was 2.22(95%CI:1.52-3.24,P<0.0001).The WMD for hemoglobin(HB) was 4.06 g/L(95%CI:-2.57-10.69,P=0.01),and the WMD for serum ferritin(SF)was 9.47μg/L(95%CI:-0.50-19.43,P<0.0001).Results were heterogeneous for all comparisons.CONCLUSION:This meta-analysis on observational studies suggests an association between H.pylori and IDA.In RCTs,eradication of H.pylori can improve HB and SF levels but not significantly. | Qu, Xin-Hua Huang, Xiao-Lu Xiong, Ping Zhu, Cui-Ying Huang, You-Liang Lu, Lun-Gen Sun, Xu Rong, Lan Zhong, Liang Sun, Da-Yu Lin, Hai Cai, Ming-Ci Chen, Zhi-Wei Hu, Bing Wu, Lian-Ming Jiang, Yi-Bin Yan, Wei-Li | 2010 | World Journal of Gastroenterology2010，16，7： | 34 |
| 4 | Galangin induces apoptosis of hepatocellular carcinoma cells via the mitochondrial pathway显示文摘AIM:To investigate the mechanism by which galangin,a polyphenolic compound derived from medicinal herbs,induces apoptosis of hepatocellular carcinoma(HCC) cells.METHODS:The 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay was used to measure cell viability.Apoptosis was evaluated by in situ uptake of propidium iodide and Hoechst 33258 and was then detected by fluorescence microscopy.Protein expressions were detected by Western blotting.To confirm the apoptotic pathway mediated by galangin,cells were transfected by bcl-2 gene to overexpress Bcl-2 or siRNA to down-regulate Bcl-2 expression.RESULTS:Galangin(46.25-370.0 μmol/L) exerted an anti-proliferative effect,induced apoptosis,and decreased mitochondrial membrane potential in a dose and time-dependent manner.Treatment with galangin induced apoptosis by translocating the pro-apoptotic protein Bax to the mitochondria,which released apoptosis-inducing factor and cytochrome c into the cytosol.Overexpression of Bcl-2 attenuated galangin-induced HepG2 cell apoptosis,while decreasing Bcl-2 expression enhanced galangin-induced cell apoptosis.CONCLUSION:Our data suggests that galangin mediates apoptosis through a mitochondrial pathway,and may be a potential chemotherapeutic drug for the treatment of HCC. | Hai-Tao Zhang Liu-Bo Lan Da-Hua Fan Kai-Dan Zhu Xiao-Yi Chen Min Wen Hui-Ming Liu Guangdong Province China Hui Luo | 2010 | World Journal of Gastroenterology2010，16，27： | 32 |
| 5 | Differentiation profile of brain tumor stem cells:a comparative study with neural stem cells显示文摘大脑肿瘤干细胞(BTSC ) 的区别侧面的理解,在肿瘤房间人口之中的关键的,将通过有神经干细胞(NSC ) 的比较借卓见进 glioma 的起源并且最终产出新途径与这难处理的疾病作斗争。这里,我们从外科的 glioma 标本的有教养、净化的 BTSC 和 NSCsfrom 人的胎儿的大脑织物,并且进一步分析了他们的细胞的生物行为,他们的特别区别性质。是期望,区分进成熟神经显型的 NSC。然而, BTSC 展出了的 Inthe 一样的区别状况区分了差别。词法上,房间变得变平并且属于第一个星期,但是逐渐地此后聚集了并且改过漂浮的肿瘤范围。在相应时期期间,无差别的房间标记 CD133 和巢在的表示率在 BTSC 不停地减少,但是 1 个星期以后,他们重获上升趋势。有趣地,区分的房间标记 GFAP 和 beta-tubulinIIIshowed 表示变化逆到无差别的房间标记的。总起来说, BTSCswere 揭示了拥有一个能力抵抗区别,它实际上代表 glioma 的恶意的行为。 | Quan Bin Zhang Xiao Yan Ji Qiang Huang Jun Dong Yu De Zhu Qing Lan | 2006 | Cell Research2006，16，12： | 34 |
| 6 | New Classification of Herlyn-Werner-Wunderlich Syndrome显示文摘 | Lan Zhu Na Chen Jia-Li Tong Wei Wang LeiZhang Jing-He Lang | 2015 | Chinese Medical Journal2015，，2： | 30 |
| 7 | Progress in China's Unconventional Oil & Gas Exploration and Development and Theoretical Technologies显示文摘The new century has witnessed a strategic breakthrough in unconventional oil & gas.Hydrocarbon accumulated in micro-/nano-scale pore throat shale systems has become an important domain that could replace current oil & gas resources.Unconventional oil & gas plays an increasingly important role in our energy demand.Tight gas,CBM,heavy oil and asphaltic sand have served as a key domain of exploration & development,with tight oil becoming a 'bright spot' domain and shale gas becoming a 'hotspot' domain.China has made great breakthroughs in unconventional oil & gas resources,such as tight gas,shale gas,tight oil and CBM,and great progress in oil shale,gas hydrate,heavy oil and oil sand.China has an estimated(223-263)×10~8t of unconventional oil resources and(890-1260)×l0^(12)m^3 of gas resources.China has made a breakthrough for progress in unconventional oil & gas study.New progress achieved in fine-grained sedimentary studies related to continental open lacustrine basin large-scale shallow-water delta sand bodies,lacustrine basin central sandy clastic flow sediments and marine-continental fine-grained sediments provide a theoretical basis for the formation and distribution of basin central reservoir bodies.Great breakthroughs have been made in unconventional reservoir geology in respect of research methodology & technology,multi-scale data merging and physical simulation of formation conditions.Overall characterization of unconventional reservoirs via multi-method and multi-scale becomes increasingly popular and facilitates the rapid development of unconventional oil & gas geological theory,method and technology.The formation of innovative,continuous hydrocarbon accumulation theory,the establishment of the framework of the unconventional oil & gas geological theory system,and the determination of the implications,geological feature,formation mechanism,distribution rule and core technology of unconventional oil& gas geological study lays a theoretical foundation for extensive unconventional oil & gas exploration and development.Theories and technologies of unconventional oil & gas exploration and development developed rapidly,including some key evaluation techniques such as 'sweet spot zone' integrated evaluation and a six-property evaluation technique that uses hydrocarbon source,lithology,physical property,brittleness,hydrocarbon potential and stress anisotropy,and some key development &engineering technologies including micro-seismic monitoring,horizontal drilling & completion and 'factory-like' operation pattern, 'man-made reservoir' development,which have facilitated the innovative development of unconventional oil & gas.These breakthroughs define a new understanding in four aspects:①theoretical innovation;② key technologies;③ complete market mechanism and national policy support;and ④ well-developed ground infrastructure,which are significant for prolonging the life cycle of petroleum industry,accelerating the upgrade and development of theories and technologies and altering the global traditional energy structure. | ZOU Caineng YANG Zhi ZHU Rukai ZHANG Guosheng HOU Lianhua WU Songtao TAO Shizhen YUAN Xuanjun DONG Dazhong WANG Yuman WANG Lan HUANG Jinliang WANG Shufang | 2015 | Acta Geologica Sinica(English Edition)2015，89，3： | 27 |
| 8 | Fusion mechanism of 2019-nCoV and fusion inhibitors targeting HR1 domain in spike protein显示文摘Very recently,a novel coronavirus,2019-nCoV,emerged in Wuhan,China and then quickly spread worldwide,resulting in>17,388 confirmed cases and 361 deaths as of 3 February 2020,thus calling for the development of safe and effective therapeutics and prophylatics.1,2 Similar to severe acute respiratory syndrome(SARS)-CoV,2019-nCoV belongs to lineage B betacoronavirus,and it has the ability to utilize human angiotensin-converting enzyme 2(ACE2)as a receptor to infect human cells. | Shuai Xia Yun Zhu Meiqin Liu Qiaoshuai Lan Wei Xu Yanling Wu Tianlei Ying Shuwen Liu Zhengli Shi Shibo Jiang Lu Lu | 2020 | Cellular & Molecular Immunology2020，17，7： | 27 |
| 9 | Microbial flocculant and its application in environmental protection显示文摘1IntroductionAlotofvariedflocculantshavebeenusedinwastewatertreatment,dredgingdownstreamprocessingandsomeindustrialfieldincl... | Zhang Tong, Lin Zhe Department of Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China Zhu Huai lan Department of Environmental Science, East China Normal University, Shanghai 200062, China | 1999 | Journal of Environmental Sciences1999，11，1： | 25 |
| 10 | Prevalence and Factors Associated with Female Sexual Dysfunction in Beijing, China显示文摘 | Wen-Jia Lou Bo Chen Lan Zhu Shao-Mei Han Tao Xu Jing-He Lang Lei Zhang | 2017 | Chinese Medical Journal2017，，12： | 25 |
| 11 | Expenditure and financial burden for the diagnosis and treatment of colorectal cancer in China:a hospital.based,multicenter,cross-sectional survey显示文摘Background: The increasing prevalence of colorectal cancer(CRC) in China and the paucity of information about relevant expenditure highlight the necessity of better understanding the financial burden and effect of CRC diagnosis and treatment. We performed a survey to quantify the direct medical and non-medical expenditure as well as the resulting financial burden of CRC patients in China.Methods: We conducted a multicenter, cross-sectional survey in 37 tertiary hospitals in 13 provinces across China between 2012 and 2014. Each enrolled patient was interviewed using a structured questionnaire. All expenditure data were inflated to the 2014 Chinese Yuan(CNY; 1 CNY = 0.163 USD). We quantified the overall expenditure and financial burden and by subgroup(hospital type, age at diagnosis, sex, education, occupation, insurance type, household income, clinical stage, pathologic type, and therapeutic regimen). We then performed generalized linear modeling to determine the factors associated with overall expenditure.Results: A total of 2356 patients with a mean age of 57.4 years were included, 57.1 % of whom were men; 13.9% of patients had stage I cancer; and the average previous-year household income was 54,525 CNY.The overall average direct expenditure per patient was estimated to be 67,408 CNY, and the expenditures for stage Ⅰ, Ⅱ, Ⅲ, and Ⅳ disease were 56,099 CNY, 59,952 CNY, 67,292 CNY, and 82,729 CNY, respectively. Non-medical expenditure accounted for 8.3%of the overall expenditure. The 1-year out-of-pocket expenditure of a newly diagnosed patient was 32,649 CNY, which accounted for 59.9% of their previous-year household income and caused 75.0% of families to suffer an unmanageable financial burden. Univariate analysis showed that financial burden and overall expenditure differed in almost all subgroups(P < 0.05), except for sex. Multivariate analysis showed that patients who were treated in specialized hospitals and those who were diagnosed with adenocarcinoma or diagnosed at a later stage were likely to spend more,whereas those with a lower household income and those who underwent surgery spent less(all P < 0.05).Conclusions: For patients in China, direct expenditure for the diagnosis and treatment of CRC seemed catastrophic,and non-medical expenditure was non-ignorable. The financial burden varied among subgroups, especially among patients with different clinical stages of disease, which suggests that, in China, CRC screening might be cost-effective. | Hui-Yao Huang Ju-Fang Shi Lan-Wei Guo Ya-Na Bai Xian-Zhen Liao Guo-iangLiu A-Yan Mao Jian-Song Ren Xiao-Jie Sun Xin-Yu Zhu Le Wang Bing-Bing Song Ling-Bin Du Lin Zhu Ji-Yong Gong Qi Zhou Yu-Qin Liu Rong Cao Ling Mai Li Lan Xiao-Hua Sun Ying Ren Jin-Yi Zhou Yuan-Zheng Wang Xiao Qi Pei-An Lou Dian Shi Ni Li Kai Zhang Jie He Min Dai | 2017 | Chinese Journal of Cancer2017，36，8： | 25 |
| 12 | 老年患者下呼吸道医院感染与控制对策显示文摘目的探讨老年患者下呼吸道医院感染的现状,并制定控制对策。方法对158例老年下呼吸道感染患者的临床资料进行调查,分析其感染现状及危险因素。结果我院老年患者医院感染发病率为9.7%,感染部位以下呼吸道为主;158例下呼吸道医院感染老年患者均患有各种慢性消耗性原发基础疾病,痰培养病原菌以革兰阴性杆菌和真菌为主,分别占48.1%和38.9%。结论抵抗力降低、住院时间长、严重的原发基础疾病及不合理使用抗生素是老年患者医院感染的危险因素,应重视老年患者医院感染的监控管理。 | 余兰 朱艳萍 YU Lan ZHU Yan-ping | 2005 | 中华医院感染学杂志2005，15，10： | 20 |
| 13 | Role of different childbirth strategies on pelvic organ prolapse and stress urinary incontinence: a prospective study显示文摘背景:筋膜、肌肉发达的支持结构的创伤的损坏可以在分娩期间是压力尿失禁(穗) 和骨盆的机关脱垂(流行音乐) 的发展的一个主要因素。这研究的目的是有希望地在流行音乐和穗上调查不同分娩策略的角色。方法:120 个选择怀孕女人的一个总数被招募并且把组划分了成二:阴道交货组(n = 72 ) 并且选择恺撒的交货组(n = 48 ) 。这些女人与穗表被询问并且在 6 以内与流行音乐数量化离子(POP-Q ) 估计了 - 在交货以后的 8 个星期。穗的关联和有交货的策略的 POP-Q 的结果被分析。t, chi (2 ) 测试,和 Mann-Witney 测试被用于统计分析。结果:在 120 个女人之中,穗在 29 期间被开发 - 在 20 个病人(16.6%) 的 30 个妊娠的星期,谁(70%) 14 有穗的症状到 6 个星期为止产后。完全 20 (16.6%) 女人们在交货以后有穗症状。产后的穗的流行断然在 29 期间与穗的出现被相关 - 30 个妊娠的星期(P <
0.001 ) 。在阴道交货组,承受的女人的 100% 形成 6 个星期产后的尿脱垂,当 87.5% 那些在选择恺撒的交货组织发达流行音乐时(P <
0.01 ) 。在阴道交货组的一度、二度的尿脱垂的百分比分别地是 20.8% 和 79.2% ,它与在选择恺撒的交货组的那些显著地不同(64.6% 和 22.9% ;两 P = 0.000 ) 。结论:怀孕和交货在穗和流行音乐的发展起一个病因学的作用。穗的发作在迟了的怀孕期间暗示产后的穗症状的重要风险。流行音乐的流行比在恺撒的交货以后在阴道交货以后是显著地更高的。 | ZHU Lan BIAN Xu-ming LONG Yan LANG Jing-he | 2008 | Chinese Medical Journal2008，，3： | 18 |
| 14 | Traditional Chinese medicinal herbs combined with epidermal growth factor receptor tyrosine kinase inhibitor for advanced non-small cell lung cancer: a systematic review and meta-analysis显示文摘BACKGROUND: Epidermal growth factor receptor tyrosine kinase inhibitor(EGFR-TKI) targeted treatment has been a standard therapy for advanced non-small cell lung cancer(NSCLC), but it is not tolerated well by all patients. In China, some studies have reported that traditional Chinese medicinal herbs(TCMHs) may increase effi cacy and reduce toxicity when combined with EGFR-TKI, but outside of China few studies of this kind have been attempted. OBJECTIVE: This study is intended to systematically review the existing clinical evidence on TCMHs combined with EGFR-TKI for treatment of advanced NSCLC. SEARCH STRATEGY: PubMed, the Cochrane Library, the Excerpta Medica Database(EMBASE), the China BioMedical Literature(CBM), and the China National Knowledge Infrastructure(CNKI) and web site of the American Society of Clinical Oncology(ASCO), the European Society for Medical Oncology(ESMO), the World Conference of Lung Cancer(WCLC) were searched; the search included all documents published in English or Chinese before October 2013. INCLUSION CRITERIA: We selected randomized controlled trials based on specifi c criteria, the most important of which was that a TCMH plus EGFR-TKI treatment group was compared with an EGFR-TKI control group in patients with advanced NSCLC. DATA EXTRACTION AND ANALYSIS: The modifi ed Jadad scale was used to assess the quality of studies. For each included study, patient characteristics, treatment details, therapeutic approach and clinical outcomes were collected on a standardized form. When disagreements on study inclusion or data extracted from a study emerged, the consensus of all coauthors provided the resolution. The clinical outcome metrics consisted of objective response rate(ORR; complete response + partial response divided by the total number of patients), disease control rate(DCR; complete response + partial response + no change divided by the total number of patients), survival rate, improved or stabilized Karnofsky performance status(KPS), and severe toxicity. RevMan 5.0 software was used for data syntheses and analyses. Risk ratio(RR) and 95% confidence interval(CI) were calculated; if the hypothesis of homogeneity was not rejected(P>0.1, I2<50%), the fi xed-effect model was used to calculate the summary RR and the 95% CI. Otherwise, a random-effect model was used.RESULTS: In this review, 19 studies were included based on the selection criteria. Of them, 13studies were of high quality and 6 studies were of low quality, according to the modifi ed Jadad scale. When the TCMH plus EGFR-TKI treatment groups were compared with the EGFR-TKI control groups the meta-analysis demonstrated a statistically signifi cant higher ORR(RR 1.34; 95% CI 1.15 to 1.57; P=0.000 2), DCR(RR 1.18; 95% CI 1.09 to 1.27; P<0.000 1), one-year survival rate(RR 1.21; 95% CI 1.01 to 1.44; P=0.04), 2-year survival rate(RR 1.91; 95% CI 1.26 to 2.89; P=0.002) and improved or stable KPS(RR 1.38; 95% CI 1.26 to 1.51; P<0.000 01). Severe toxicity for rash was decreased(RR 0.55; 95% CI 0.32 to 0.94; P=0.03), as were nausea and vomiting(RR 0.17; 95% CI 0.04 to 0.72; P=0.02) and diarrhea(RR 0.46; 95% CI 0.24 to 0.89; P=0.02). Sensitivity analysis indicated that fi ndings of the meta-analysis were robust to study quality. In the funnel plot analysis, asymmetry was observed, and publication bias was indicated by Egger's test(P=0.03).CONCLUSION: TCMH intervention can increase effi cacy and reduce toxicity when combined with EGFR-TKI for advanced NSCLC, although this result requires further verifi cation by more well designed studies. | Zhong-liang Liu Wei-rong Zhu Wen-chao Zhou Hai-feng Ying Lan Zheng Yuan-biao Guo Jing-xian Chen Xiao-heng Shen | 2014 | Journal of Integrative Medicine2014，12，4： | 18 |
| 15 | Dihydromyricetin alleviates carbon tetrachloride-induced acute liver injury via JNK-dependent mechanism in mice显示文摘AIM: To assess the effects of dihydromyricetin(DHM) as a hepatoprotective candidate in reducing hepatic injury and accelerating hepatocyte proliferation after carbon tetrachloride(CCl4) treatment.METHODS: C57 BL/6 mice were used in this study. Mice were orally administered with DHM(150 mg/kg) for 4 d after CCl4 treatment. Serum and liver tissue samples were collected on days 1, 2, 3, 5 and 7 after CCl4 treatment. The anti-inflammatory effect of DHM was assessed directly by hepatic histology detection and indirectly by serum levels of aspartate aminotransferase(AST), alanine aminotransferase(ALT), albumin, and superoxide dismutase(SOD). Inflammatory cytokines, such as interleukin(IL)-1β, IL-6 and tumor necrosis factor-α(TNF-α), were detected using ELISA kits. Proliferating cell nuclear antigen(PCNA) staining was used to evaluate the role of DHM in promoting hepatocyte proliferation. Hepatocyte apoptosis wasmeasured by TUNEL assay.Furthermore,apoptosis proteins Caspases-3,6,8,and 9 were detected by Western blot.SP600125 were used to confirm whether DHM regulated liver regeneration through JNK/TNF-αpathways.RESULTS:DHM showed a strong anti-inflammatory effect on CCl4-induced liver injury in mice.DHM could significantly decrease serum ALT,AST,IL-1β,IL-6 and TNF-αand increase serum albumin,SOD and liver SOD compared to the control group after CCl4 treatment(P<0.05).PCNA results indicated that DHM could significantly increase the number of PCNA positive cells compared to the control(348.9±56.0 vs 107.1±31.4,P<0.01).TUNEL assay showed that DHM dramatically reduced the number of apoptotic cells after CCl4 treatment compared to the control(365.4±99.4 vs 90.5±13.8,P<0.01).Caspase activity detection showed that DHM could reduce the activities of Caspases-8,3,6 and 9 compared to the control(P<0.05).The results of Western blot showed that DHM increased the expression of JNK and decreased TNF-αexpression.However,DHM could not affect TNF-αexpression after SP600125 treatment.Furthermore,DHM could significantly improve the survival rate of acute liver failure(ALF)mice(73.3%vs 20.0%,P<0.0001),and SP600125 could inhibit the effect of DHM.CONCLUSION:These findings demonstrate that DHM alleviates CCl4-induced liver injury,suggesting that DHM is a promising candidate for reversing liver injury and ALF. | Jun Xie Jie Liu Tu-Ming Chen Qing Lan Qing-Yu Zhang Bin Liu Dong Dai Wei-Dong Zhang Li-Ping Hu Run-Zhi Zhu | 2015 | World Journal of Gastroenterology2015，21，18： | 17 |
| 16 | Transmutation of ancient settlements and environmental changes between 6000-2000 aBP in the Chaohu Lake Basin, East China显示文摘Based on the temporal-spatial distribution features of ancient settlement sites from the middle and late Neolithic Age to the Han dynasty in the Chaohu Lake Basin of Anhui Province,East China,using the methods of GIS combined with the reconstructed paleoenvironment by the records of lake sediment since Holocene,the transmutation of ancient settlements with response to environmental changes in this area has been discussed.Studies show that the main feature of transmutation of ancient settlements from the middle and late Neolithic Age to the Han Dynasty was that the distribution of settlements in this area changed from high altitudes to low ones and kept approaching the Chaohu Lake with the passage of time.These could be the response to the climate change from warm-moist to a relatively warm-dry condition during the middle Holocene,leading to the lake level fluctuations.The large area of exposed land provided enough space for human activities.These indicate that the above changes in geomorphologic evolution and hydrology influenced by climate conditions affected the transmutation of ancient settlements greatly.The distribution pattern of settlement sites was that the number of sites in the west was more than in the east.This pattern may be related to the geomorphologic conditions such as frequent channel shifting of the Yangtze River as well as flood disasters during the Holocene optimum.Therefore,climate change was the inducement of the transmutation of ancient settlements in the Chaohu Lake Basin,which exerted great influence on the distribution,expansion and development of the ancient settlements. | WU Li WANG Xinyuan ZHOU Kunshu MO Duowen ZHU Cheng GAO Chao ZHANG Guangsheng LI Lan LIU Li HAN Weiguang | 2010 | Journal of Geographical Sciences2010，20，5： | 16 |
| 17 | Holocene environmental change and archaeology,Yangtze River Valley,China:Review and prospects显示文摘Holocene 环境变化和环境考古学是学习人地球的相互作用系统的一个国际工程的重要部件。这篇论文在最后三十年在长江山谷考察 Holocene 环境变化和环境考古学研究的进步,那包括大淡水湖, Holocene 违反和海平面的变化的进化, Holocene 气候变化和东方亚洲人季风变化,在原始文明和环境变化,文化打断和 palaeoflood 事件的上升和秋天之间的关系,以及在 agri 的起源之间的关系这些研究部件是由标明日期建立地区性的环境、文化的进化的一张年表的湖的沉积,石笋和泥炭的 underpinned。学科交差并且另外的环境代理指示物需要在长江山谷的上面、中间、更低的活动范围在考古学的地点形成和自然沉积环境的比较研究被过去常。现代技术象遥感,分子的 bioarchaeology ,和虚拟现实那样,应该综合与当前过去常标明日期,在环境考古学macro-studies,的分析的 geochemical , sedimentological ,和 palaeobotanical 方法并且微学习,以便在长江山谷区域提供更大的全面卓见进 Holocene 环境、文化的相互作用和变化。 | Li Wu Feng Li Cheng Zhu Lan Li Bing Li | 2012 | Geoscience Frontiers2012，3，6： | 16 |
| 18 | Trocar-site hernia after gynecological laparoscopic surgery: a 20-year, single-center experience显示文摘Background:Trocar-site hernia(TSH)is a serious complication after laparoscopic procedures.Although it is a rare entity,it have life-threatening consequences.This study aimed to retrospectively analyze the potential associated factors for TSH following gynecologic laparoscopy and summarize prevention strategies based on our experience.Methods:We searched for gynecological laparoscopic surgeries in the medical records system of Peking Union Medical College Hospital(PUMCH)from August 1998 to July 2018 and further sifted through the results for cases involving TSH.All included patients were divided into different groups according to patient characteristics,and the rate of TSH was compared among groups.Moreover,the detailed information of all patients with TSH was recorded and analyzed.Statistical analyses were performed with GraphPad Prism 6.Results:The approximate total rate of post-operative TSH among gynecologic laparoscopy procedures performed at PUMCH in the last 20 years was 0.016%(9/55,244).The rate of TSH was significant higher in elder patients(>60 years old;3/2686,0.112%)than in younger patients(<60 years old,6/52,558;0.011%,P=0.008).Moreover,the approximate rate of TSH was significantly higher after single-incision laparoscopic surgery(SILS,2/534,0.357%)than conventional laparoscopic surgery(7/54,710,0.013%,P=0.003).The average age of patients with TSH was 53.4 years(range,35.0-79.0 years).Two of the nine patients had late-onset TSH following SILS;the other seven had early-onset TSH following conventional laparoscopy.Five TSH cases occurred at right lateral port sites,and the remaining three occurred at the umbilical port site.All patients underwent repair surgery,and one required small bowel resection.Conclusion:Older age and SILS are potential associated factors for TSH development,while reducing excessive manipulation and improving suturing skills,especially at the umbilical trocar site following SILS and the right lateral trocar site,can avoid herniation. | Ya-Pei Zhu Shuo Liang Lan Zhu Zhi-Jing Sun Jing-He Lang | 2019 | Chinese Medical Journal2019，，22： | 16 |
| 19 | Simultaneous detection of different serum pepsinogens and its primary application显示文摘AIM: To develop the simple, rapid and sensitive dual-label time-resolved fluoroimmunoassay for pepsinogens in human serum.METHODS: Based on two-site sandwich protocol, mono-clonal antibodies (McAbs) against pepsinogen Ⅰ (PG Ⅰ) and PG Ⅱ were co-coated in 96 microtitration wells, and tracer McAbs against PG Ⅰ and PG Ⅱ were labeled with europium (Eu) and samarium (Sm) chelate, respectively. Diluted serum samples of Eu3+- and Sm3+-McAbs were added into microtitration wells simultaneously. After washing, fluorescence of bound Sm3+ and Eu3+ tracers was detected. RESULTS: The detection limit was 0.2 μg/L for PG Ⅰ and 0.05 μg/L for PG Ⅱ. The assay range was 5.0-320.0 μg/Lfor PG Ⅰ and 1.0-55.0 μg/L for PG Ⅱ. The average re-covery rate was 102.7% for PG Ⅰ and 98.8% for PG Ⅱ. Sera from healthy controls and patients with gastric dis-ease were analyzed. The PG detected by dual-label as-say was in good agreement with that detected by single-label assay or by enzyme-linked immunosorbent assay. CONCLUSION: Dual-label assay can provide high-throughput serological screening for gastric diseases. | Zhang, Jue Guo, Ji-Zhong Xiao, Hua-Long Zhu, Lan Liu, Hai-Yan Zhang, Yi Huang, Biao | 2010 | World Journal of Gastroenterology2010，16，24： | 15 |
| 20 | Histopathological classification and follow-up analysis of chronic atrophic gastritis显示文摘BACKGROUND The pathological diagnosis and follow-up analysis of gastric mucosal biopsy have been paid much attention,and some scholars have proposed the pathological diagnosis of 12 kinds of lesions and accompanying pathological diagnosis,which is of great significance for the treatment of precision gastric diseases,the improvement of the early diagnosis rate of gastric cancer,and the reduction of missed diagnosis rate and misdiagnosis rate.AIM To perform a histopathological classification and follow-up analysis of chronic atrophic gastritis(CAG).METHODS A total of 2248 CAG tissue samples were collected,and data of their clinical characteristics were also gathered.Based on these samples,the expression levels of Mucin 1(MUC1),MUC2,MUC5AC,and MUC6 in CAG tissue were tested by immunohistochemical assay.Moreover,we followed these patients for up to four years.The difference between different stages of gastroscopic biopsy was observed.RESULTS Through observation,it is believed that CAG should be divided into four types,simple type,hyperplasia type,intestinal metaplasia(IM)type,and intraepithelial neoplasia(IEN)type.Simple CAG accounted for 9.1%(205/2248),which was more common in elderly people over 60 years old.The main change was that the lamina propria glands were reduced in size and number.Hyperplastic CAG accounted for 29.1%(654/2248),mostly occurring between 40 and 60 years old.The main change was that the lamina propria glands were atrophy accompanied by glandular hyperplasia and slight expansion of the glands.IM CAG accounted for 50.4%(1132/2248),most of which increased with age,and were more common in those over 50 years.The atrophy of the lamina propria glands was accompanied by significant IM,and the mucus containing sialic acid or sulfate was distinguished according to the nature of the mucus.The IEN type CAG accounted for 11.4%(257/2248),which developed from the previous types,with severe gland atrophy and reduced mucus secretion,and is an important precancerous lesion.CONCLUSION The histological typing of CAG is convenient to understand the property of lesion,determine the follow-up time,and guide the clinical treatment. | Yang-Kun Wang Lan Shen Tian Yun Bin-Feng Yang Chao-Ya Zhu Su-Nan Wang | 2021 | World Journal of Clinical Cases2021，9，16： | 15 |
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