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	题名	作者	年代	出处	被引量
1	Three-dimensional perfused human in vitro model of nonalcoholic fatty liver disease显示文摘AIM To develop a human in vitro model of non-alcoholic fatty liver disease(NAFLD), utilising primary hepatocytes cultured in a three-dimensional(3D) perfused platform. METHODS Fat and lean culture media were developed to directly investigate the effects of fat loading on primary hepatocytes cultured in a 3D perfused culture system. Oil Red O staining was used to measure fat loading in the hepatocytes and the consumption of free fatty acids(FFA) from culture medium was monitored. Hepatic functions, gene expression profiles and adipokine release were compared for cells cultured in fat and lean conditions. To determine if fat loading in the system could be modulated hepatocytes were treated with known anti-steatotic compounds. RESULTS Hepatocytes cultured in fat medium were found to accumulate three times more fat than lean cells and fat uptake was continuous over a 14-d culture. Fat loading of hepatocytes did not cause any hepatotoxicity and significantly increased albumin production. Numerous adipokines were expressed by fatty cells and genes associated with NAFLD and liver disease were upregulated including: Insulin-like growth factorbinding protein 1, fatty acid-binding protein 3 and CYP7A1. The metabolic activity of hepatocytes cultured in fatty conditions was found to be impaired and the activities of CYP3A4 and CYP2C9 were significantlyreduced, similar to observations made in NAFLD patients. The utility of the model for drug screening was demonstrated by measuring the effects of known antisteatotic compounds. Hepatocytes, cultured under fatty conditions and treated with metformin, had a reduced cellular fat content compared to untreated controls and consumed less FFA from cell culture medium.CONCLUSION The 3D in vitro NAFLD model recapitulates many features of clinical NAFLD and is an ideal tool for analysing the efficacy of anti-steatotic compounds.	Tomasz Kostrzewski Terri Cornforth Sophie A Snow Larissa Ouro-Gnao Cliff Rowe Emma M Large David J Hughes	2017	World Journal of Gastroenterology2017，23，2：	6
2	Suppression of host immune response by the core protein of hepatitis C virus: possible implications for hepatiits C virus persistence显示文摘	Large M K Kittlesen D J Hahn Y S	1999	J Immunol1999，162，2：	2
3	Multilayer Reflectors in Animals Using Green and Gold Beetles as Con- trasting Examples显示文摘	PARKER A R MCKENZIE D R LARGE M C J	1998	J Exp Biol1998，201，：	1
4	The danger of dangerousness : why we must remove the dangerousness criterion from our mental health acts 显示文摘	LARGE M M RYAN C J NEII-SSEN O B HAYES R A	2008	Journal of Medical Ethics2008，34，12：	1
5	High dynamic range characterization of the trauma patient plasma proteome显示文摘	Liu T Qian W J Gritsenko M A Xiao W Moldawer L L Kaushal A Monroe M E Vamum S M Moore R J Purvine S O Maier R V Davis R W Tompkins R G Camp D G 2nd Smith R D and the Inflammation and the Host Response to Injury Large Scale Collaborative Research Programm		0，，10：	1
6	Mental health acts that require dangerousness for involuntary admission may delay the initial treatment of schizophrenia 显示文摘	LARGE M M NEILSSEN O B RYAN C J	2008	Soc Psychiatry Psychiatr Epidemiol2008，，43：	1
7	Microstructured polymer optical fibre 显示文摘	Van eijkelenborg M A Large M C J Argyros A	2001	Optics Express2001，，9：	1
8	The role of viscoelastic properties in strain testing using microstructured polymer optical fibres 显示文摘	LARGE M C J MORAN J YE L	2009	Measurement Science and Technology2009，20，3：	1
9	Microstructured polymer optical fibre 显示文摘	VAN EIJKELENBORG M A LARGE M C J ARGYROS A	2001	Optics Express2001，9，7：	1
10	Incidence,risk factors,and complications of postoperative delirium in elderly patients undergoing radical cystectomy显示文摘	Large M C Reichard C Williams J T B	2013	Urology2013，81，1：	1
11	An unusual pulsar-PSR 0826-34显示文摘	Durdin J M Large M I Little A G	2005	MNRAS2005，186，：	1
12	Microstructured polymer optical fibre 显示文摘	VAN EIJKELENBORG M A LARGE M C J ARGYROS A	2001	Opt Express2001，9，7：	1
13	Imidazopyridine derivatives as inhibitors of aurora kinases:lead optimization studies toward the identification of an orally bioavailable preclinical development candidate显示文摘	BAVETSIAS V LARGE J M SUN C	2010	J Med Chem2010，53，14：	1
14	The influence of climate, hy- drology and permafrost on Holocene peat accumulation at 3500 m on the eastern Qinghai Tibetan Plateau 显示文摘	Large D J Spiro B Fen'at M	2009	Quaternary Science Reviews2009，28，：	1
15	Solution doping of microstructured polymer optical fibres 显示文摘	LARGE M C J PONRATHNAM S ARGYROS A	2004	Opt Express2004，12，9：	1
16	Structure-based design of imidazopyrazine derivatives as selective inhibitors of Aurora-A kinase in cells显示文摘	BOULOC N LARGE J M KOSMOPOULOU M	2010	Bioorg Med Chem Lett2010，20，20：	1
17	Hollow-core rnicrostructured polymer optical fiber 显示文摘	Argyros A Eijkelenborg M A Large M C J	2006	Opt Lett2006，31，：	1
18	Microstructured polymeroptical fibre 显示文摘	VAN EIJKELENBORG M A LARGE M C J ARGYROS A	2001	Optics Express2001，9，7：	1
19	Liquid-filled hollow core microstructured polymer optical fiber 显示文摘	COX F M ARGYROS A LARGE M C J	2006	Opt Express2006，14，：	1
20	Muhilayer reflectors in animals using green and gold beetles as contrasting examples显示文摘	PARKER A R MCKENZIE D R LARGE M C J	1998	J Exp Biol1998，201，：	1

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